Search for Camptothecin-yielding Ophiorrhiza species from southern Western Ghats in India: A HPTLC-densitometry study

Rajan, R. Govinda; Varghese, Sibi Chirakkadamoolayil; Kurup, Rajani; Gopalakrishnan, Roja; Venkataraman, Ramesh; Satheeshkumar, K.; Baby, Sabulal

doi:10.1016/j.indcrop.2012.07.054

Cited by 46 publications

(13 citation statements)

References 12 publications

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“…In the study, CPT production by the original parent strain OmF3 under the uninduced condition was found to be CPT is a quinoline alkaloid with potent anticancer activity isolated originally from C. acuminata by Wall et al 34 This has also been detected from other plant resources like Ophiorrhiza species. 35,36 Due to the pharmaceutical importance of CPT, alternative sources for its production is significant. At the same time, several endophytes such as Entrophospora infrequens, Neurospora sp., Trichoderma sp., Fusarium solani, and so on have already been characterized for CPT production.…”

Section: Enhancement Of Cpt Production By Np Supplementationmentioning

confidence: 99%

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Aswani

Jasim

Vishnu

et al. 2020

Biotechnology Progress

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In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC-MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. However, the fragmentation masses with m/z of 320, 305, 277 and 220 specific to the CPT could be identified only for the OmF3 and OmF4. These CPT producing fungi were further identified as Meyerozyma sp. OmF3 and Talaromyces sp. OmF4. The cultures of these two fungi were then supplemented with nanoparticles and analyzed for the quantitative enhancement of CPT production by LC-MS/MS. From the result, Meyerozyma sp. OmF3 was found to produce 947.3 ± 12.66 μg/L CPT, when supplemented with 1 μg/ mL zinc oxide nanoparticles and the same for uninduced parental strain OmF3 was only 1.77 ± 0.13 μg/L. At the same time, Talaromyces sp. OmF4 showed the highest production of 28.97 ± 0.37 μg/L of CPT when cultured with 10 μg/mL silver nanoparticles and the same for uninduced strain was 1.19 ± 0.24 μg/L. The observed quantitative enhancement of fungal CPT production is highly interesting as it is a rapid and cost effective method. The study is remarkable due to the identification of novel fungal sources for CPT production and its enhancement by nanoparticle supplementation.

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Section: Enhancement Of Cpt Production By Np Supplementationmentioning

confidence: 99%

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Aswani

Jasim

Vishnu

et al. 2020

Biotechnology Progress

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“…Significant variations of camptothecin amounts were noticed between the ecotypes/genotypes of the similar Ophiorrhiza species or variety collected from different geographic locations. This study used in the selection of the high amount of camptothecin in ophiorrhiza genotypes or ecotypes for commercial applications .…”

Section: Natural Drugsmentioning

confidence: 99%

High‐performance thin‐layer chromatography: An economical alternative for the quality control of medicinal plants and derived products

Kumar

Sharma

2018

Separation Science Plus

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Currently, delicious and essential items are intentionally diluted to gain higher profit in the market. Awareness about the quality of naturally derived products such as food, nutrients, cosmetics, and drugs has increased. An economical method for their quality assessment is needed. In this regard, high‐performance thin‐layer chromatography is an inexpensive, fast, and reproducible technique for quality control. The basics of thin‐layer chromatography and high‐performance thin‐layer chromatography have been reviewed previously, but a comprehensive compilation of its applications is lacking. The current review focuses on high‐performance thin‐layer chromatography based analytical methods for the quality assessment of various plant species, plant materials, plant‐derived products, food and nutrients, and cosmetics (natural origin). This review covers all aspects of high‐performance thin‐layer chromatography and will prove fruitful for research and development in academia and industry.

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“…Growth (fresh weight) and CPT content were determined in the in vitro-derived rooted individual shoots, rooted shoot clusters, same aged natural seedlings planted in the net-pots, field-grown plants before flowering (4-5 months old), and plants at flowering stage (6 months old). All the samples were air dried separately and CPT was quantified using HPLC using a method described earlier (19). Quantitation was done by comparing the standard curve plotted using different concentrations of authentic CPT (Sigma Aldrich, USA), each taken in triplicate.…”

Section: Culture Initiationmentioning

confidence: 99%

“…prostrata (17), O. eraiantha (18), among others. However, maximum CPT was reported from O. mungos and it has been recognized as a potential source of CPT (12,19). Considering the growing demand for CPT together with the importance of conservation of Ophiorrhiza species, development of alternate strategies for the production of the compound is significant (20,21).…”

Section: Introductionmentioning

confidence: 99%

Tissue culture of Ophiorrhiza mungos L., a prospective method for the production of an anticancer drug, camptothecin

et al. 2018

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Camptothecin (CPT), a cytotoxic quinoline indole alkaloid, is an anticancer compound. Its two major semi synthetic derivatives, topotecan and irinotecan, are Food and Drug Administration (FDA) approved drugs effectively used for treating different cancer types and sold under the trade names Hycamtin and Camptosar. Among the Ophiorrhiza species, Ophiorrhiza mungos contains the highest CPT level (0.02 % g dw). CPT level was determined in plants before flowering (0.074 ± 0.003 % g dw) and at flowering (0.052 ± 0.002 % g dw). Multiple shoot cultures were induced on seedling-derived explants of O. mungos in half strength MS solid media supplemented with 1.0 mg/L BAP to obtain 12.00 ± 1.22 shoots in 20 days. Repeated subcultures at 20 days interval yielded 20.00 ± 3.71 shoots/subculture of shoot clusters. After elongation, rooting and transplanting, the growth of shoot clusters were studied in both in vitro and same aged naturally grown seedlings. Highest biomass (4.62 ± 0.158 g fw) was obtained in in vitro-derived shoot clusters. CPT increased according to biomass and the maximum CPT was recorded in in vitro rooted shoot clusters after 15 days (0.031 ± 0.001 % g dw). Hence, in vitro-derived rooted shoot clusters of O. mungos cultivated in net-pots for 60 days under shade net house conditions found to be a sustainable source for CPT.

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Search for Camptothecin-yielding Ophiorrhiza species from southern Western Ghats in India: A HPTLC-densitometry study

Cited by 46 publications

References 12 publications

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

High‐performance thin‐layer chromatography: An economical alternative for the quality control of medicinal plants and derived products

Tissue culture of Ophiorrhiza mungos L., a prospective method for the production of an anticancer drug, camptothecin

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