We investigate the possibility of detecting single sqaurk production at the proposed LHeC collider, in the framework of R-parity violating supersymmetry. Taking advantage of the enhancement of the direct resonance production of squark and the distinctive kinematics distributions of q → lq two body decay final states, the LHeC provides excellent opportunities of probing R-violating L Q D interactions at unprecedented level compared to all the knowledge derived from indirect low energy nucleon measurements. If no apparent deviation from SM predictions on high invariant mass of muon and b-quark final states at the LHeC with 1f b −1 data, the sensitivities on L Q D coupling constant λ ′ 131 × λ ′ 233 can be improved by nearly four orders, at energy scale about 100 GeV .