Search for New Antileishmanial Chemotherapeutics

Abstract

Objective: The objective of this work was to screen a number of compounds for their antileishmanial efficacy and cytotoxicity profiling.Methods: Curry leaf oil, cypress oil and spikenard oil were identified by gas chromatography-mass spectrometry (GC/MS) analysis. Betulinic acid, spikenard oil, cypress oil and curry leaf oil were evaluated for their in vitro antileishmanial activity against Leishmania donovani AG83 wild-type, sodium stibogluconate resistant (SSG-resistant), paromomycin (PMM-resistant) and GE1 field type strains on axenic and cellular amastigote model and compared the results with standard drugs used to treat leishmaniasis. Results:Betulinic acid showed strong antileishmanial activity against wild-type (SI= 192.8), SSG-resistant (SI= 19.3) and GE1 strains (SI= 100), whereas cypress oil has produced highest antileishmanial activity against PMM-resistant strains (SI= 15.09) among all the tested drugs. The data obtained also revealed that cypress oil had the maximum CC50 Conclusion: All tested drugs had antileishmanial property but among them, betulinic acid possess strong antileishmanial activity in case of both wild-type and drug-resistant leishmaniasis.value of 452.9 μl among all standard and tested drugs.

Trypanocidal, Anti-Leishmanial, and Cytotoxic Activity of Muehlenbeckia Tamnifolia (Kunth) Meins (Polygonaceae)

“…Das et al found that lupeol has significant anti-Leishmania activity with IC 50 values of 65 and 15 μg/ml compared to promastigote and amastigote, respectively [21]. Other triterpenic compounds, such as betulinic acid, have anti-leishmanial activity, activity that can be related to the inhibition of topoisomerase [22,23].…”