Search for the presence of six Mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus

Kovacic, Rémi; Launay, Valérie; Tuppin, Philippe; Lafeuillade, Alain; Feuillie, V.; Montagnier, Luc; Grau, Odile

doi:10.1128/jcm.34.7.1808-1810.1996

Cited by 25 publications

(17 citation statements)

References 22 publications

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“…M. fermentans DNA also was reported in the PBMC of 8% healthy controls, 5.8% in HIV infected patients and in 15% of patients with sexually transmitted diseases [20]. In the present study the percent positive PCR for M. fermentans in control subjects is in complete agreement with the study by Kovacic et al [20].…”

Section: Discussionsupporting

confidence: 92%

“…fermentans DNA also was reported in the PBMC of 8% healthy controls, 5.8% in HIV infected patients and in 15% of patients with sexually transmitted diseases [20]. In the present study the percent positive PCR for M. fermentans in control subjects is in complete agreement with the study by Kovacic et al [20]. We believe that the prevalence of this organism in HIV infected patients and sexually transmitted diseases was underestimated by these investigators since the majority of these patients were under antimicrobial treatment.…”

Section: Discussionmentioning

confidence: 90%

“…However, in a recent study based on antibiotic resistance [19], it was suggested that one particular strain of M. fermentans was, in fact, a tissue culture contaminant and was not derived from the patients [19]. Moreover, M. fermentans DNA was recently reported in 8% of healthy persons, in 5.8% of AIDS patients and in 15% of patients with sexually transmitted diseases [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome

Vojdani

1998

FEMS Immunology and Medical Microbiology

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Mycoplasma fermentans and other Mycoplasma species are colonizers of human mucosal surfaces and may be associated with human immunodeficiency virus infection. While many infectious agents have been described in different percentages of patients with Chronic Fatigue Syndrome (CFS), little is known about the prevalence of mycoplasmas and especially M. fermentans in CFS patients. A polymerase chain reaction (PCR)-based assay was used to detect Mycoplasma genus and M. fermentans genomes in peripheral blood mononuclear cells (PBMC) of CFS patients. Blood was collected from 100 patients with CFS and 50 control subjects. The amplified products of 717 bp of Mycoplasma genus, and 206 bp of M. fermentans were detected in DNA purified from blood samples in 52% and 34% of CFS samples, respectively. In contrast, these genomes were found in only 14% and 8% of healthy control subjects respectively (P 6 0.0001). All samples were confirmed by Southern blot with a specific probe based on internal sequences of the expected amplification product. Several samples, which were positive for Mycoplasma genus, were negative for M. fermentans indicating that other Mycoplasma species are involved. A quantitative PCR was developed to determine the number of M. fermentans genome copies present in 1 Wg of DNA for controls and CFS patients. Mycoplasma copy numbers ranging from 130 to 880 and from 264 to 2400 were detected in controls and CFS positive subjects, respectively. An enzyme immunoassay was applied for the detection of antibodies against p29 surface lipoprotein of M. fermentans to determine the relationship between M. fermentans genome copy numbers and antibody levels. Individuals with high genome copy numbers exhibited higher IgG and IgM antibodies against M. fermentans specific peptides. Isolation of this organism by culture from clinical specimens is needed in order to demonstrate specificity of signal detected by PCR in this study. z

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome

Vojdani

1998

FEMS Immunology and Medical Microbiology

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“…Thus, it is possible that M. fermentans can influence pathogenesis in co-infection with other viruses. M. fermentans is detected in approximately 10% of HIV-seronegative individuals [8], suggesting that a certain percentage of the healthy population is infected with M. fermentans. The present study was performed to examine whether pathogenicity is increased by co-infection with M. fermentans and SARS-CoV using an in vitro cell culture system.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans

et al. 2007

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We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual infection completely killed cells and prevented the establishment of persistent SARS-CoV infection. M. fermentans induced inhibition of cell proliferation, but the cells remained alive. Apoptosis was induced easily in M. fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis.

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“…Most strains of M. fermentans infecting humans are difficult to cultivate in cell-free media, and the development of the polymerase chain reaction (PCR) with suitable primers has made its detection much easier. M. fermentans has been discovered in the throat, urine or peripheral blood cells of up to 36% of human immunodeficiency virus (HIV) patients; [18][19][20] the organism was not detected in healthy controls but Katseni et al 21 found it in 30% of HIV seronegative patients attending a clinic for sexually transmitted diseases and Kovacic et al 22 reported it in 11% of the peripheral blood mononuclear cells from HIV seronegative subjects. Chingbingyong and Hughes 23 found the organism in the saliva of 44% of normal people.…”

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

The importance of B‐cells and ecto‐5′nucleotidase in Mycoplasma fermentans infection and the relevance to rheumatoid arthritis

2007

Immunology

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Summary The aim of this work was to discover if Mycoplasma fermentans, which is known to infect B cells, could be the cause of the raised ecto‐5′‐nucleotidase observed in the synovial fluid of rheumatoid arthritis patients. The ecto‐5′‐nucleotidase activity in the patients' serum has been shown to correlate with the erythrocyte sedimentation rate and DNA from the mycoplasma has been found in the synovial fluid. B lymphoblastoid cell lines were exposed to 16 strains of Mycoplasma fermentans and their ecto‐5′‐nucleotidase, CD73, was measured both biochemically and by mouse antibodies to human ecto 5′‐nucleotidase using the fluorescence activated cell sorter. The type strain, PG 18, did not grow with the B cells. Some of the mycoplasma strains (9/15) increased the cellular ecto‐5′‐nucleotidase activity from twice to 17 fold, and usually showed 5′‐nucleotidase activity themselves. At least one strain, M106, induced human 5′‐nucleotidase on the normally 5′‐nucleotidase negative Daudi and Raji Burkitt's lymphoma cell lines, and increased sevenfold the 5′‐nucleotidase on the monocyte/macrophage cell line THP‐1. Growing the cells in aged medium increased the level of mycoplasma infection. This mycoplasma‐induced enzyme showed a conformational change and an increase in activity with a glycosylation change involving mannose groups. The other group of strains, mostly of respiratory or cell culture origin, usually did not have any 5′‐nucleotidase of their own and decreased the B‐cell enzyme activity by about half. Electron microscopy and flow cytometry showed that the strain M106 was filamentous and could be found inside the B‐cells. The 5′‐nucleotidase‐inducing strains of M. fermentans may be important in the aetiology of rheumatoid arthritis.

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Search for the presence of six Mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus

Cited by 25 publications

References 22 publications

Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome

Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome

Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans

The importance of B‐cells and ecto‐5′nucleotidase in Mycoplasma fermentans infection and the relevance to rheumatoid arthritis

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