Searching for and Revealing the System of Pleistocene Refugia for the Species Pinus sylvestris L.

Sannikov, S. N.; Петрова, И. В.; Егоров, Е. В.; Sannikova, N. S.

doi:10.1134/s1067413620030133

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The significant differentiation between North Transvolga's populations and Pre-Ural's populations (29%) is possibly due to the history of dispersal of the species in the Pleistocene. Despite the fact that the South Ural refugium occupies a dominant position in both groups, it makes a much larger contribution to the Ural's population than to the North Transvolga's populations [47,48].…”

Section: Population Genetic Structurementioning

confidence: 98%

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

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In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

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Section: Population Genetic Structurementioning

confidence: 98%

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

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“…The identified diff erentiation of populations and plus gene pools of Scots pine of different geographical origins may be the result of the intersection of the species' migration routes in the post-glacial period. Specifically, with the allozyme analysis, the authors discovered that five different Pleistocene refugia could have participated in creating the gene pool of Scots pine populations on the East European Plain (Sannikov et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Variability of Scots pine (<i>Pinus sylvestris</i> L.) plus trees in the Middle and Upper Volga Region with the use of ISSR markers

Sheikina,

Romanov

2024

Vestn. VOGiS

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One of the serious issues in forest breeding is how to reduce the variability level in breeding populations of forest tree species that is a set of selected plus trees. The problem is that variability is jeopardized by the risk of losing the genetic diversity of future artificial forests, as well as emerging inbreeding depression in the seed plus trees progeny. DNA markers are an effective tool to study variability, identify features of the genetic structure and degree of plant differentiation. The research focuses on assessing the level of the genetic diversity and the degree of differentiation of plus trees of various geographic origin with the use of ISSR markers. We used six ISSR primers to study 270 plus trees grown in the Penza region, the Chuvash Republic, the Republic of Tatarstan and the Mari El Republic. The samples of plus trees under study were characterized by different levels of genetic diversity. Two hundred fifteen PCR fragments were identified for six ISSR primers in total, while the number of amplified fragments varied from 186 to 201 in different plus trees samples. The genetic variabil ity varied within the following limits: 95.7–96.9 %, polymorphic loci; 1.96–1.97, the number of alleles per locus; 1.31–1.48, the number of effective alleles per locus: finally, 0.291–0.429, Shannon’s index; 0.205–0.298, the expected heterozygosity. According to the analysis of molecular variance (AMOVA), 82 % of the variability of ISSR markers is typical for the plus tree samples, while only 18 % is variability among the compared groups of trees from different geographical zones. The dendrogram generated by UPGMA showed that the plus trees grown in the Penza region, the Chuvash Republic and the Republic of Tatarstan are similar in term of the genetic structure of plus trees, while the plus gene pool of Scots pine from the Mari El Republic stands alone. The results of the research prove that the level of genetic diversity, the structure of genetic variability, and the nature of differentiation of plus trees are consistent with those previously elicited for natural populations of Scots pine in the Middle and Upper Volga region.

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“…Significant differentiation between the populations from the East European Plain and the populations from the Middle Urals (28%) may be related to the history of the distribution of the species in the Pleistocene. Populations of the Middle Urals were settled mainly from the South Ural refugium, while this refugium made a smaller contribution to the gene pools of the East European populations [45,46].…”

Section: Population Genetic Structure Of P Sylvestrismentioning

confidence: 99%

Genetic Diversity, Structure, and Differentiation of Pinus sylvestris L. Populations in the East European Plain and the Middle Urals

Sboeva

Chertov

Nechaeva

et al. 2022

Forests

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Genetic diversity is important for the long-term survival of species and plays a critical role in their conservation. To manifest the adaptive potential, it is necessary to preserve the allelic diversity of populations, including both typical and region-specific alleles. Molecular genetic analysis of 22 populations of Scotch pine (Pinus sylvestris L.; Pinaceae) in 10 subjects of the Russian Federation in the East European Plain and the Middle Urals was carried out. Molecular genetic analysis of 22 populations of P. sylvestris revealed 182 polymorphic PCR fragments. The studied populations are characterized by a medium level of genetic diversity. A high subdivision coefficient (GST) of the studied populations was established; the intensity was 0.559. At the same time, the level of subdivision differed for different regions; for the populations from the Middle Urals, it was 15.5% (GST = 0.155), and for the populations from the East European Plain, it was 55.8% (GST = 0.558). The dendrogram of genetic similarity shows five clusters of the studied populations of P. sylvestris according to their geographical location. The populations from the East European Plain are mostly characterized by typicality, while the populations from the Middle Urals, on the contrary, are more specific in gene pools. The use of the coefficient of genetic originality to identify populations with typical and specific alleles allows for solving the problem of selecting populations for the conservation of forest genetic resources. The data obtained on genetic diversity, and the structure of populations growing in areas of active logging, are important for determining the geographical origin of plant samples, which is an integral part of the control of illegal logging.

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Searching for and Revealing the System of Pleistocene Refugia for the Species Pinus sylvestris L.

Cited by 5 publications

References 15 publications

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Variability of Scots pine (<i>Pinus sylvestris</i> L.) plus trees in the Middle and Upper Volga Region with the use of ISSR markers

Genetic Diversity, Structure, and Differentiation of Pinus sylvestris L. Populations in the East European Plain and the Middle Urals

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