Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS

Ligor, Tomasz; Zawadzka, Joanna; Strączyński, G.; Paredes, Rosa María González; Wenda-Piesik, Anna; Rațiu, Ileana-Andreea; Muszytowski, Marek

doi:10.3390/molecules26071817

Cited by 11 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In rat experiments, Meinardi et al found that the content of 2-pentanone in the breath of rats with chronic kidney disease and acute kidney injury induced by surgery was higher than that of control group. In human disease research, compared with healthy people, 2-pentanone is higher in urine of patients with glomerular disease and idiopathic membranous nephropathy . The increased content of 2-pentanone in breath and urine may be due to abnormal metabolism in the kidney.…”

Section: Resultsmentioning

confidence: 99%

HS–SPME–GC–MS Untargeted Analysis of Normal Rat Organs Ex Vivo: Differential VOC Discrimination and Fingerprint VOC Identification

Liu

Zhou

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

The investigation of volatile organic compounds (VOCs) in human metabolites has been a topic of interest as it holds the potential for the development of non-invasive technologies to screen for organ lesions in vivo. However, it remains unclear whether VOCs differ among healthy organs. Consequently, a study was conducted to analyze VOCs in ex vivo organ tissues obtained from 16 Wistar rats, comprising 12 different organs. The VOCs released from each organ tissue were detected by the headspace-solid phase microextraction-gas chromatography-mass spectrometry technique. In the untargeted analysis of 147 chromatographic peaks, the differential volatiles of rat organs were explored based on the Mann–Whitney U test and fold change (FC > 2.0) compared with other organs. It was found that there were differential VOCs in seven organs. A discussion on the possible metabolic pathways and related biomarkers of organ differential VOCs was conducted. Based on the orthogonal partial least squares discriminant analysis and receiver operating characteristic curve, we found that differential VOCs in the liver, cecum, spleen, and kidney can be used as the unique identification of the corresponding organ. In this study, differential VOCs of organs in rats were systematically reported for the first time. Profiles of VOCs produced by healthy organs can serve as a reference or baseline that may indicate the presence of disease or abnormalities in the organ’s function. Differential VOCs can be used as the fingerprint of organs, and future integration with metabolic research may contribute to the development of healthcare.

show abstract

Section: Resultsmentioning

confidence: 99%

HS–SPME–GC–MS Untargeted Analysis of Normal Rat Organs Ex Vivo: Differential VOC Discrimination and Fingerprint VOC Identification

Liu

Zhou

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Headspace GC analyzes only the vapor phase above the sample; thus, the additional derivatization stage is unnecessary. Metabolite concentrations in the vapor phase are low, and thus high sensitivity and mass resolution time-of-flight mass spectrometry (TOF-MS) is chosen [ 36 , 37 ]. The derivatization step can also help overcome low sensitivity and enhance signals.…”

Section: Analytical Methods In Ckd Metabolomics Studiesmentioning

confidence: 99%

“…Furthermore, resolution and sensitivity enhancement could be achieved by different MS instrumentation. The prevalent amount of CKD metabolomic works were made using time-of-flight mass spectrometry (TOF-MS) [ 23 , 36 , 39 , 42 ] and quadrupole time-of-flight mass spectrometry (Q-TOF-MS) [ 43 , 44 , 45 , 46 ], which is characterized by high sensitivity, fast scanning speeds, and accurate mass measurements. However, in one of the works, both Q-TOF-MS and reconstitution techniques were performed [ 40 ].…”

Section: Analytical Methods In Ckd Metabolomics Studiesmentioning

confidence: 99%

CKD Urine Metabolomics: Modern Concepts and Approaches

Danilova,

Maslova,

Stavrianidi

et al. 2023

Pathophysiology

View full text Add to dashboard Cite

One of the primary challenges regarding chronic kidney disease (CKD) diagnosis is the absence of reliable methods to detect early-stage kidney damage. A metabolomic approach is expected to broaden the current diagnostic modalities by enabling timely detection and making the prognosis more accurate. Analysis performed on urine has several advantages, such as the ease of collection using noninvasive methods and its lower protein and lipid content compared with other bodily fluids. This review highlights current trends in applied analytical methods, major discoveries concerning pathways, and investigated populations in the context of urine metabolomic research for CKD over the past five years. Also, we are presenting approaches, instrument upgrades, and sample preparation modifications that have improved the analytical parameters of methods. The onset of CKD leads to alterations in metabolism that are apparent in the molecular composition of urine. Recent works highlight the prevalence of alterations in the metabolic pathways related to the tricarboxylic acid cycle and amino acids. Including diverse patient cohorts, using numerous analytical techniques with modifications and the appropriate annotation and explanation of the discovered biomarkers will help develop effective diagnostic models for different subtypes of renal injury with clinical applications.

show abstract

“…SPME extraction, desorption, and thermal fiber cleaning were carried out automatically by means of an MPS-2 autosampler. The fiber and sample conditions were applied according to those utilized in earlier experiments [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Analysis of VOCs in Urine Samples Directed towards of Bladder Cancer Detection

et al. 2022

Self Cite

View full text Add to dashboard Cite

Bladder cancer is one of most common types of cancer diagnosed in the genitourinary tract. Typical tests are costly and characterized by low sensitivity, which contributes to a growing interest in volatile biomarkers. Head space solid phase microextraction (SPME) was applied for the extraction of volatile organic compounds from urine samples, and gas chromatography time of flight mass spectrometry (GC×GC TOF MS) was used for the separation and detection of urinary volatiles. A cohort of 40 adult patients with bladder cancer and 57 healthy persons was recruited. Different VOC profiles were obtained for urine samples taken from each group. Twelvecompounds were found only in the samples from theBC group.The proposed candidate biomarkers are butyrolactone; 2-methoxyphenol; 3-methoxy-5-methylphenol; 1-(2,6,6-trimethylcyclohexa-1,3-dien-1-yl)-2-buten-1-one; nootkatone and 1-(2,6,6-trimethyl-1-cyclohexenyl)-2-buten-1-one.Since most of the studies published in the field are proving the potential of VOCs detected in urine samples for the screening and discrimination of patients with bladder cancer from healthy, but rarely presenting the identity of proposed biomarkers, our study represents a novel approach.

show abstract

Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS

Cited by 11 publications

References 21 publications

HS–SPME–GC–MS Untargeted Analysis of Normal Rat Organs Ex Vivo: Differential VOC Discrimination and Fingerprint VOC Identification

HS–SPME–GC–MS Untargeted Analysis of Normal Rat Organs Ex Vivo: Differential VOC Discrimination and Fingerprint VOC Identification

CKD Urine Metabolomics: Modern Concepts and Approaches

Analysis of VOCs in Urine Samples Directed towards of Bladder Cancer Detection

Contact Info

Product

Resources

About