Searching for tagged male-sterile mutants of arabidopsis

Glover, Julie; Blömer, Katherina C.; Farrell, Leigh B.; Chaudhury, Abed; Dennis, E. S.

doi:10.1007/bf02673365

Cited by 9 publications

(19 citation statements)

References 21 publications

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“…Furthermore, as shown in this paper, ms35 is not allelic to another male sterility mutation found in the T-DNA mutagenized line 1926, which may cause anther indehiscence under certain growth conditions (Glover et al, 1996).…”

Section: supporting

confidence: 52%

“…Such mutants have been obtained in many species of higher plants (Kaul, 1988 ;Goldberg et al, 1993). In the selfpollinating species Arabidopsis thaliana, many ms mutants have been generated by chemical and irradiation mutagenesis (Van der Veen & Wirtz, 1968 ;Regan & Moffatt, 1990 ;Chaudhury et al, 1992Chaudhury et al, , 1994Dawson et al, 1993 ;Preuss et al, 1993), by T-DNA insertional mutagenesis (Feldmann et al, 1994 ;Glover et al, 1996 ;Park et al, 1996) and transposon mutagenesis .…”

Section: mentioning

confidence: 99%

“…The following lines of Arabidopsis thaliana L. were obtained from the Nottingham Arabidopsis Stock Centre : A. thaliana ecotype Landsberg erecta (Ler, NW20) ; the chromosome 3 marker line hy2, gl1, cer7 (NW132) ; the multimarker line W100F (N2224, carries nine mutations an, ap1, er, py, hy2, gl1, bp, cer2, tt3 distributed over the five pairs of Arabidopsis chromosomes, Koornneef et al, 1987) ; and a male sterile mutant line generated by T-DNA mutagenesis and reported as a dehiscence mutant (N2338, Glover et al, 1996). The recessive male sterile mutant ms35 was generated in our laboratory by Xray mutagenesis of Ler seeds (Dawson et al, 1993).…”

Section: Plants and Growth Conditionsmentioning

confidence: 99%

“…The ms line 1926 was generated by T-DNA insertional mutagenesis and was reported to have indehiscent anthers (Glover et al, 1996). A test for allelism between ms35 and ' ms1926 ' was carried out by crossing the latter as a male sterile homozygote with the ms35iLer heterozygote.…”

Section: Test For Allelism Between Ms35 and A T-dnaassociated Male Stmentioning

confidence: 99%

“…Allelism tests were conducted between ms35 and a T-DNA mutagenized line 1926 of Arabidopsis that was reported as indehiscent (Glover et al, 1996). All the F " plants derived from crosses between ms35 (heterozygote) and homozygous male sterile 1926 plants were male fertile.…”

Section: Allelism Tests To T-dna Line 1926mentioning

confidence: 99%

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Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

et al. 1999

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The male sterile mutant, ms35, of Arabidopsis thaliana was produced by X-irradiation of seeds. The mutant produces fertile pollen, but is male sterile because the anthers do not dehisce. Anther development in ms35 plants occurs as in wild-type Arabidopsis until shortly after microspores are released from meiotic tetrads. Thereafter, in the wild type, bands of lignified, cellulosic secondary wall thickenings are laid down around the cells of the anther endothecium. In contrast, wall thickenings are not formed in the endothecium of the ms35 mutant. Development of other lignified tissues, for example the vascular tissue of the stamen, occurs normally in ms35 plants. In mutant anthers, as pollen maturation is completed, the stomium is cleaved but the anther wall does not retract to release pollen. The block in anther dehiscence in ms35 plants is specifically correlated with the absence of endothecial wall thickenings. The ms35 mutation represents the first genetic evidence in support of the proposed role of the endothecium in anther dehiscence. The ms35 gene was mapped to the top arm of chromosome 3 (hy2-(4.17p 2.31 cM)-ms35-(32.14p5.45 cM)-gl1).

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Section: supporting

confidence: 52%

Section: mentioning

confidence: 99%

Section: Plants and Growth Conditionsmentioning

confidence: 99%

Section: Test For Allelism Between Ms35 and A T-dnaassociated Male Stmentioning

confidence: 99%

Section: Allelism Tests To T-dna Line 1926mentioning

confidence: 99%

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Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

et al. 1999

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A homologue of the yeast HOP1 gene is inactivated in the Arabidopsis meiotic mutant asy1

et al. 2000

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Synapsis of homologous chromosomes is a key event in meiosis as it is essential for normal chromosome segregation and is implicated in the regulation of crossover frequency. We have previously reported the identification and cytological characterisation of a T-DNA-tagged asynaptic mutant of Arabidopsis thaliana. We have demonstrated that this mutant, asy1, is defective in meiosis in both males and females. Cloning and nucleotide sequencing of the ASY1 gene has revealed that it encodes a polypeptide of 596 amino acids that exhibits similarity to the HOP1 gene of Saccharomyces cerevisiae, which is known to encode a protein essential for synaptonemal complex assembly and normal synapsis. Expression studies indicate that, in common with a number of other Arabidopsis meiotic genes, ASY1 exhibits low-level expression in a range of plant tissues. Southern analysis coupled with database searching has resulted in the identification of an ASY1 homologue, ASY2. Although asy1 exhibits a strong asynaptic phenotype, a residual low level of synapsis indicates that ASY1 and ASY2 may exhibit a low degree of functional redundancy.

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MEI1 , an Arabidopsis gene required for male meiosis: isolation and characterization

Mascarenhas

1998

Sexual Plant Reproduction

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The T-DNA tagged mutant gene of Arabidopsis thaliana, mei1, produces after meiosis an abnormal tetrad, consisting of five to eight microspores of varying sizes and DNA contents. Plant DNA flanking the inserted T-DNA was isolated by inverse PCR. An approximately 16-kb DNA fragment spanning the T-DNA insertion site was isolated by screening a wild-type genomic library, using the plant flanking DNA as a probe. Using RT-PCR and RNA isolated from very young flower buds, a cDNA fragment was obtained. Nucleotide sequence comparison of the cDNA and the genomic sequence in this region indicated a gene which contained two introns. The 5′ and 3′ splice sites of neither intron comply with the :GU...AG: rule. In the mutant, the T-DNA had inserted into one of the introns. The deduced sequence of the MEI1 wild-type gene, which contains 89 amino acids, shows possible similarity with the human acrosin-trypsin inhibitor, HUSI-II, and is about the same size. Two wildtype DNA fragments, both extending over the T-DNA insertion site, were introduced into mutant plants by Agrobacterium-mediated transformation and plants were selected for both hygromycin and kanamycin resistance. Several independent male-fertile transformants were obtained with one of the DNA fragments. The fragment showing complementation of the mutant phenotype indicated that the sequence with similarity to the acrosintrypsin inhibitor is MEI1. Within the 16-kb genomic fragment two other genes were identified; one showed no overall similarity to any protein sequence in the database and the other had almost complete identity with an Arabidopsis-transcribed sequence tag with similarity to ACC oxidase. Double mutants between mei1 and qrt1 were made, permitting better characterization of the mei1 phe-notype because the individual microspores continued to be held together after callose dissolution.

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Searching for tagged male-sterile mutants of arabidopsis

Cited by 9 publications

References 21 publications

Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

A homologue of the yeast HOP1 gene is inactivated in the Arabidopsis meiotic mutant asy1

MEI1 , an Arabidopsis gene required for male meiosis: isolation and characterization

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