Searching pathogenic bacteria in the rare biosphere of the ocean

Doni, Lapo; Martı́nez-Urtaza, Jaime; Vezzulli, Luigi

doi:10.1016/j.copbio.2023.102894

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Besides being isolated in diseased and moribund oysters, V. aestuarianus can also be found in coastal water as free‐living forms, in marine sediments and associated with fishes and different environmental substrates (e.g., plankton organisms, marine aggregates, chitin particles), showing a seasonal trend correlated mainly with temperature (Azandégbé et al, 2010; Vezzulli, Pezzati, et al, 2015; Vezzulli, Stauder, et al, 2015). Sediment, particulate matter and planktonic organisms also represent environmental reservoirs for V. aestuarianus that, as previously shown for Vibrio cholerae and other vibrios (Doni et al, 2023; Huq et al, 2005; Pruzzo et al, 2008), can survive in these compartments for long time, possibly forming biofilms, also during unfavourable environmental conditions (Vezzulli, Pezzati, et al, 2015; Vezzulli, Stauder, et al, 2015).…”

Section: Introductionmentioning

confidence: 65%

Plankton and marine aggregates as transmission vectors for V. aestuarianus 02/041 infecting the pacific oyster Crassostrea gigas

Doni,

Tassistro,

Oliveri

et al. 2023

Environ Microbiol Rep

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Vibrio aestuarianus is a bacterium related to mass mortality outbreaks of the Pacific oyster, Crassostrea gigas in Europe. In this study, the role of different planktonic substrates (phytoplankton cells, marine aggregates and chitin fragments) in mediating V. aestuarianus 02/041 infection of oysters was evaluated by controlled infection experiments. It was shown that phytoplankton cells and, to a greater extent, marine aggregates, significantly promote V. aestuarianus 02/041 intake by C. gigas maintained under stressful conditions in the laboratory. Such intake is associated with higher concentration of the pathogen in the bivalve hemolymph and compromised health status of infected oysters. In contrast, chitin particles do not play a significant role as transmission vector for V. aestuarianus 02/041 infecting its bivalve host. Interestingly, incorporation into marine aggregates foster extracellular proteases (ECPs) activity and a higher expression of bacterial virulence genes, that are potentially involved in bivalve infection. Results from this study contribute to elucidate transmission patterns of V. aestuarianus 02/041 to C. gigas that may be useful for the development of efficient measures to prevent and control oyster disease outbreaks.

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Section: Introductionmentioning

confidence: 65%

Plankton and marine aggregates as transmission vectors for V. aestuarianus 02/041 infecting the pacific oyster Crassostrea gigas

Doni,

Tassistro,

Oliveri

et al. 2023

Environ Microbiol Rep

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“…The biosensor showed the detection results in planktonic and benthic environmental samples similar to that with the qPCR and colorimetric assay methods. Apart from the toxic algae, marine pathogens, including viruses, bacteria and protozoa, can cause the contamination of seafood, finally resulting in human infections [163,164]. Some pathogens originate from natural marine waters, such as the cholera bacterium and Vibrio cholera [165], while some others, such as fecal streptococci and escherichia coli, originate from defecation/ urination/shedding of human or animal hosts and are carried via rivers or sewage effluent into coastal water [166].…”

Section: Sensors For Detecting Marine Microorganismsmentioning

confidence: 99%

“…Some pathogens originate from natural marine waters, such as the cholera bacterium and Vibrio cholera [165], while some others, such as fecal streptococci and escherichia coli, originate from defecation/ urination/shedding of human or animal hosts and are carried via rivers or sewage effluent into coastal water [166]. For measuring these path- Apart from the toxic algae, marine pathogens, including viruses, bacteria and protozoa, can cause the contamination of seafood, finally resulting in human infections [163,164]. Some pathogens originate from natural marine waters, such as the cholera bacterium and Vibrio cholera [165], while some others, such as fecal streptococci and escherichia coli, originate from defecation/urination/shedding of human or animal hosts and are carried via rivers or sewage effluent into coastal water [166].…”

Section: Sensors For Detecting Marine Microorganismsmentioning

confidence: 99%

A Review of Marine In Situ Sensors and Biosensors

Liu

Cui

2023

JMSE

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The exploration of the ocean is essential for the exploitation of marine resources and the sustainable development of human society. In order to assess both the health and the resources of the marine environment, a variety of chemical and biological sampling is needed. Traditionally, marine samples are collected on site and transported to a laboratory for analysis. Analytical methods are often tedious, and it is difficult to know the in situ real-time status. This review provides a comprehensive summary of the development of in situ chemical and biological sensors for the typical compounds in the ocean, including methane, radon, ferrous ion, carbon dioxide, microorganisms, pollutants, nutrients and seafood. Different types of sensors for each compound are highlighted, such as novel electrochemical and optical sensors. Commercial status of different sensors is introduced, and performance of representative sensors is compared and discussed deeply. The advantages and disadvantages of each sensing technique are analyzed and evaluated in detail. Finally, future prospects and work directions are presented, such as the deployment of these in situ sensors on fixed and/or moving platforms, development of microfluidic sensors and exploration of new antifouling materials and methods. This paper could serve as a resource for developing more advanced in situ chemical sensors and biosensors for marine scientific research, as well as related practical applications for monitoring marine resource exploration and exploitation and for environmental protection.

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“…Of the different bacteria that can be found at deep-sea vents, members of the Vibrio genus are among those who can adapt to a wider range of conditions, as they are ubiquitous in different environments, including seawater, sediments, and associated with marine organisms ( Doni et al 2023 a; Doni et al (2023) ). In particular, piezo-tolerant Vibrio strains have been previously isolated from deep-sea environments ( Raguénès et al 1997 ; Hasan et al 2015 ), including hydrothermal vents ( Hasan et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Pan-Genome Provides Insights into Vibrio Evolution and Adaptation to Deep-Sea Hydrothermal Vents

Bosi,

Taviani,

Avesani

et al. 2024

Genome Biology and Evolution

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This study delves into the genomic features of 10 Vibrio strains collected from deep-sea hydrothermal vents in the Pacific Ocean, providing insights into their evolutionary history and ecological adaptations. Through sequencing and pan-genome analysis involving 141 Vibrio species, we found that deep-sea strains exhibit larger genomes with unique gene distributions, suggesting adaptation to the vent environment. The phylogenomic reconstruction of the investigated isolates revealed the presence of 2 main clades: The first is monophyletic, consisting exclusively of Vibrio alginolyticus, while the second forms a monophyletic clade comprising both Vibrio antiquarius and Vibrio diabolicus species, which were previously isolated from deep-sea vents. All strains carry virulence and antibiotic resistance genes related to those found in human pathogenic Vibrio species which may play a wider ecological role other than host infection in these environments. In addition, functional genomic analysis identified genes potentially related to deep-sea survival and stress response, alongside candidate genes encoding for novel antimicrobial agents. Ultimately, the pan-genome we generated represents a valuable resource for future studies investigating the taxonomy, evolution, and ecology of Vibrio species.

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Searching pathogenic bacteria in the rare biosphere of the ocean

Cited by 6 publications

References 26 publications

Plankton and marine aggregates as transmission vectors for V. aestuarianus 02/041 infecting the pacific oyster Crassostrea gigas

Plankton and marine aggregates as transmission vectors for V. aestuarianus 02/041 infecting the pacific oyster Crassostrea gigas

A Review of Marine In Situ Sensors and Biosensors

Pan-Genome Provides Insights into Vibrio Evolution and Adaptation to Deep-Sea Hydrothermal Vents

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