Season- and sex-related variation in mucin secretions of the striped Venus clam, <i>Chamelea gallina</i> (Linnaeus, 1758) (Bivalvia: Veneridae)

Guglielmi, Marco Vito; Semeraro, Daniela; Mentino, Donatella; Mastrodonato, María; Mastrototaro, Francesco; Scillitani, Giovanni

doi:10.1080/24750263.2023.2190343

Cited by 4 publications

(1 citation statement)

References 58 publications

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“…In mollusks, mucus is secreted by many different cell types (Bolognani‐Fantin & Vigo, 1967; Davies & Hawkins, 1998; Pawlicki et al, 2004; Shirbhate & Cook, 1987; Simkiss & Wilbur, 1977), and its composition varies in relation to biological (e.g., growth, feeding, locomotion, and reproduction) and environmental parameters like humidity, salinity, and temperature (Accogli et al, 2017; Davies et al, 1990; Guglielmi, Semeraro, Mentino, et al, 2023; Petraccioli et al, 2013). Because of its rheological properties, the mucus produced by the foot of gastropods is involved in several functions such as adhesion, movement, mating, osmoregulation, protection against injuries and pathogens, trail‐tracing and interactions with the microbiota (Cilia & Fratini, 2018; Davies & Hawkins, 1998; Lai et al, 2010; McFarlane, 1980; Smith, 2010).…”

Section: Introductionmentioning

confidence: 99%

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Guglielmi,

Mastrodonato,

Semeraro

et al. 2024

Microscopy Res & Technique

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Aluminum (Al) is used in everyday life and present in food drugs, packaging, industry, and agriculture. Although it is the most common metal in the Earth crust, a correlation has been demonstrated between its presence and various pathologies, even serious ones, especially of a neurological type. However, there is a histological gap regarding the role Al can have in contact with the covering and secreting epithelia. The alterations of the ventral and dorsal foot mucocytes and their secretions of the snail Eobania vermiculata caused by Al were investigated in situ by histochemical and lectin‐histochemical techniques. Administration to different experimental groups took place for 3 and 9 days with 50 and 200 μM of AlCl3. Several types of mucocytes were detected with a prevalent secretion of acid glycans in the foot of E. vermiculata. Sulfated glycans prevail in the dorsal region, with one type showing only fucosylated residues and another also having galactosaminylated and glycosaminylated residues. Carboxylated glycans prevail in the ventral region, with presence of galactosaminylated, glycosaminylated, and fucosylated residuals in both cells. Snails treated presented a general decrease of mucin amount in the secreting cells and affected the mucus composition. These changes could alter the rheological and functional properties of the mucus with possible implications for the health of the treated animals.Research Highlights Snails were fed with Al‐contaminated lettuce at different concentrations. In the foot mucocytes produced mucus with prevailing acidic glycans. In the treated resulted a reduction in the amount of mucus and an alteration of glycan composition.

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Section: Introductionmentioning

confidence: 99%

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Guglielmi,

Mastrodonato,

Semeraro

et al. 2024

Microscopy Res & Technique

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Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

Mentino,

Nicchia,

Frigeri

et al. 2024

Microscopy Res & Technique

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Aquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin‐4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4‐deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4‐deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5‐μm thick, were analyzed by histochemical methods (Periodic acid‐Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA‐I, AAA, LTA). Immunohistochemical methods such as anti‐Muc6 for neck cells and anti‐ β‐ H+/K+‐ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions.Research Highlights Glycopatterns in gastric glands were compared between wild type (WT) and AQP4‐deficient knockout (KO) mice by histochemical and lectin‐binding methods. In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed. In the parietal cells lower fucosylation also resulted. AQP4‐deficiency affects glycosylation and could result in altered functionality and pathological conditions.

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First data on the effect of Aluminium intake in Chamelea gallina of exploited stocks in the Southern Adriatic Sea (Central Mediterranean Sea)

Guglielmi

Semeraro

Ricci

et al. 2023

Regional Studies in Marine Science

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Season- and sex-related variation in mucin secretions of the striped Venus clam, Chamelea gallina (Linnaeus, 1758) (Bivalvia: Veneridae)

Cited by 4 publications

References 58 publications

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

First data on the effect of Aluminium intake in Chamelea gallina of exploited stocks in the Southern Adriatic Sea (Central Mediterranean Sea)

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