Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak [Tecomella undulata (Sm.) Seem]

Chhajer, Sidhika; Kalia, Rajwant K.

doi:10.1007/s11738-017-2364-2

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…Similar synergism of auxin and cytokinin(s) for efficient shoot amplification has been reported in Salvadora oleoides (Phulwaria et al 2014), Tinospora cordifolia (Panwar et al 2018), and Moringa oleifera (Gupta et al 2020). The replacement of sucrose with table sugar did not affect the multiplication rate/response in this study as evidenced by Chhajer and Kalia (2017).…”

Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Osupporting

confidence: 86%

“…Explants collected during the spring season were better in terms of percentage bud-breaking response, early bud-breaking, or days to respond and showed the least contamination in this study. Similarly, season-specific culture responses were earlier observed in Tecomella undulata (Chhajer and Kalia 2017) and Tinospora cordifolia (Panwar et al 2018). Funada et al (2001) explained that during the spring season, more auxins are synthesized in young buds which trigger cellular division in the cambium and hence assist in enhancing active growth.…”

Section: Resultsmentioning

confidence: 65%

“…In comparison with 0.25, 0.5, or 1.0 mg L −1 BAP alone, greater numbers of shoots were obtained from cultures grown on medium containing combinations of BAP (0.25, 0.5, or 1.0 mg L −1 ) and Kin (0.25 or 0.5 mg L −1 ) ( Table 2). Similar is the case in Tecomella undulata where a combination of two cytokinins proved beneficial in comparison with a single cytokinin (Chhajer and Kalia 2017). Shoots subcultured on MS basal medium without any PGR failed to respond and turned black/brown within 2 to 3 wk.…”

Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Omentioning

confidence: 52%

“…2a). In additives, adenine sulfate may have complemented the effect of cytokinins owing to its base structural similarity to cytokinins (Chhajer and Kalia 2017). Likewise, addition of 15 mg L −1 adenine sulfate improved multiple shoot formation in Withania somnifera (Sivanandhan et al 2015).…”

Section: Effect Of Cytokinins On Bud-breakingmentioning

confidence: 99%

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An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

Patel

Lodha

Shekhawat

2020

In Vitro Cell.Dev.Biol.-Plant

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Mitragyna parvifolia (Roxb.) Korth., commonly known as "Kadam," is an endangered and pharmaceutically valued tree of the family Rubiaceae. The numerous medicinal properties are attributed to the various alkaloids of this plant. Poor seedling survival (due to very small size of seeds, approximately 10,000 per gm), overexploitation and habitat destruction are the major constraints in conserving the wild stocks of this species. This paper reports a significant, improved, and repeatable micropropagation protocol of M. parvifolia using nodal explants of a mature tree. Nodal explants harvested during spring season from the lopped tree differentiated the maximum number of axillary shoots (5.3 ± 0.82 per node) on full-strength Murashige and Skoog (MS) medium containing 3.0 mg L −1 6-benzylaminopurine (BAP) and additives (25 mg L −1 each of adenine sulfate, L-arginine, and citric acid and 50 mg L −1 ascorbic acid). Shoots were amplified in vitro through (1) recurrent transfer of mother explants and (2) subculturing on fresh nutrient medium. The greatest number of shoots (13.4 ± 1.26) with an average length of 6.2 ± 1.03 cm was produced after 4 wk on MS medium containing 0.5 mg L −1 BAP, 0.25 mg L −1 kinetin (Kin), 0.1 mg L −1 Indole-3-acetic acid (IAA), additives, 100 mg L −1 activated charcoal (AC), and 0.8% (w/v) agar. This is the first report of concurrent ex vitro rooting and acclimatization (CEVRA) in M. parvifolia. About 90% micropropagated shoots rooted ex vitro on pulse treatment of 500 mg L −1 Indole-3-butyric acid (IBA; for 5 min) and produced 8.5 ± 0.97 roots per shoot with an average length of 9.40 ± 1.06 cm, after 5 wk. Over 80% of CEVRA plantlets were successfully transplanted to the soil in field. The defined protocol can be employed for conservation ex situ and restoration/rehabilitation/reintroduction in situ of M. parvifolia. Keywords Endangered tree. Ex situ conservation. Ex vitro rooting. Micropropagation. Mitragyna parvifolia This paper is dedicated to the frontline workers of COVID-19 Pandemic.

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Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Osupporting

confidence: 86%

Section: Resultsmentioning

confidence: 65%

Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Omentioning

confidence: 52%

Section: Effect Of Cytokinins On Bud-breakingmentioning

confidence: 99%

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An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

Patel

Lodha

Shekhawat

2020

In Vitro Cell.Dev.Biol.-Plant

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“…Among these, Micropropagation also known as in vitro propagation, potentially offering breakthrough in clonal mass production of recalcitrant species, has been attempted widely in forest trees and particularly in desert teak several times during past three decades. However, so far, nearly all the attempts have focused on utilizing organogenesis pathway using nodal or internodal segments taken from mature tree stands or in vitro propagules, and/or callus derived from them as explant (Raj Bhansali, 1993;Nandwani et al, 1996;Robinson et al, 2005;Aslam et al, 2009;Tyagi & Tomar, 2013;Chhajer and Kalia, 2017). Despite valuable efforts, organogenic regeneration pathway has not yielded a satisfactory propagation protocol for this species since it has many limitations, the most important one is very low adventitious rooting ability of the explants resulting in poor regeneration e ciency.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Rastgoo

2021

Preprint

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Over-exploitation of trees by increasing human population has put an enormous pressure especially on those species having slow growth and low reproduction rate. Desert teak (Tecomella undulata (Sm.) Seem) a multipurpose agroforestry tree of arid zone has fallen on the verge of extinction in Iran due to lack of efficient seed reproduction. Inherent low adventitious rooting has caused vegetative mass macro- and micropropagation (organogenesis pathway) of this tree to be unsuccessful. Somatic embryogenesis (SE) pathway by production of bipolar embryo capable of development into a complete plantlet can solve the problem. Hence, a research was carried out to evaluate the feasibility of in vitro regeneration of the tree by SE. Ovary extracted from un-pollinated flower was cultured in the media supplemented with different auxins and cytokinins (CKs). The results showed α-naphthalene acetic acid (NAA) was superior in induction of embryogenic callus (EC). NAA ranging 5.4–21.5 µM could induce the highest ECs which exhibited developing pro-embryogenic masses (PEMs) and globular somatic embryos. Both CKs, thidiazuron (TDZ) and N6-benzyladenine (BA), though induced good callogenesis at low concentrations but the formed calli were non-embryogenic. Proliferation of NAA–induced ECs revealed that hormone-free medium is the best choice. However, the media containing 40.5 and 54 µM NAA alone could also induce somatic embryos along with callus proliferation. Effort for maturation of the obtained globular, hear-shaped and torpedo embryos did not yield satisfactory results. Low BA-contained medium led to secondary SE. More research is expected to be undertaken to optimize SE in desert teak.

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Insight View of Topical Trends on Synthetic Seeds of Rare and Endangered Plant Species and Its Future Prospects

Nandini

Giridhar

2019

Synthetic Seeds

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Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak [Tecomella undulata (Sm.) Seem]

Cited by 15 publications

References 38 publications

An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Insight View of Topical Trends on Synthetic Seeds of Rare and Endangered Plant Species and Its Future Prospects

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