Seasonal Changes in the Newt, <i>Trituroides hongkongensis</i>, Testis. II. An Ultrastructural Study on the Lobule Boundary Cell

Tso, Ernest; Lofts, B.

doi:10.1111/j.1463-6395.1977.tb00231.x

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…In the present study, using a modified WATTENBERG'S technique, it was clearly demonstrated that (i) the reaction for the test of A5-3P-HSD is negative in the pericystic cells containing spermatogonia and spermatocytes, and Sertoli cells, (ii) a weak reaction is found in pericystic cells containing mature spermatozoa with a tendency for the reaction to be more intense in the cysts situated nearer to the glandular tissue, and (iii) a very strong reaction is found in the cells of the outer layer of glandular tissue which originates from the pericystic cells. These results are in accordance with those found in Pleurodeles waltlii (7,38), Salamandra salamandra (1 0 , 23), Trituroides hongkongensis (43) and Triturus cristatus (15,47). Such unique localization of the SH-secreting cells was also reported in certain kinds of fishes, in which the tubule boundary cells constituting the wall of the seminiferous tubules were suggested to be the source of testicular androgens (29,31).…”

Section: Pericystic Cells In the Glandular Tissuesupporting

confidence: 82%

HISTOCHEMICAL AND ELECTRON MICROSCOPIC OBSERVATIONS ON THE STEROID HORMONE‐SECRETING CELLS IN THE TESTIS OF THE JAPANESE RED‐BELLIED NEWT, CYNOPS PYRRHOGASTER PYRRHOGASTER

Imai

Tanaka

1978

Dev Growth Differ

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The testis of the Japanese red-bellied newt, Cynops pyrrhogaster pyrrhogaster, caught in the middle of May was investigated in order to demonstrate the presence of steroid hormone (SH)-secreting cells, by means of histochemical and electron microscopic techniques.The anterior part is immature and consists of many seminal cysts containing spermatogonia and spermatocytes. The posterior part is mature and made up of many cysts containing mature spermatozoa and glandular tissue.Histochemical studies revealed d5-3&hydroxysteroid dehydrogenase (d5-3/3-HSD) activity in the pericystic cells of the mature part, weak in those before spermiation, then gradually becoming more intense in the pericystic cells just after spermiation. Activity was most pronounced in the outer layer of cells of the glandular tissue which originate from the pericystic cells. In contrast to this, the dS-3,%HSD reaction was entirely negative in the pericystic cells of the immature part, the Sertoli cells, and the inner cells of the glandular tissue, originating from the Sertoli cells.Electron microscopic observation of the pericystic cells and glandular tissue gave further information on the histochemical features. The pericystic c:lls in the immature part are fibroblastlike cells. The cell organelles of these cells are poorly developed except for rough-surfaced endoplasniic reticulum and rod-shaped mitochondria. In the mature part, the fibroblast-like cells are transformed into cells characteristic of SH-secreting cells. In the cytoplasm, the amount of smooth-surfaced endoplasmic reticulum increases and typical globular mitochondria with tubular cristae take the place of the rod-shaped mitochondria. In addition, a number of lipid droplets and lipofuscin granules appear. Most of the fine structures of the outer layer of the cells of the glandular tissue were found to be similar in appearance to those of the SH-secreting cells in mammals and.other vertebrates. These results strongly suggest that, as in the other urodeles, the SH-secreting cells in the testis of the Japanese red-bellied newt are the pericystic cells in the mature part and in the outer layer of cells of the glandular tissue.The newt testis is divided into two main parts of spermatogenetic stages.

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Section: Pericystic Cells In the Glandular Tissuesupporting

confidence: 82%

HISTOCHEMICAL AND ELECTRON MICROSCOPIC OBSERVATIONS ON THE STEROID HORMONE‐SECRETING CELLS IN THE TESTIS OF THE JAPANESE RED‐BELLIED NEWT, CYNOPS PYRRHOGASTER PYRRHOGASTER

Imai

Tanaka

1978

Dev Growth Differ

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“…In an ultrastructural study on Sertoli cells in the newt Trituroides hongkongensis, it was concluded by Tso and Lofts (19776) that these cells mainly act as a support for spermatozoa bundles, phagocytize (undischarged) spermatozoa and facilitate the liberation of spermatozoa (see also van Oordt et a1 1949). In the glandular tissue of this species Sertoli cells undergo degeneration as in Ssalamandra.…”

Section: Discussionmentioning

confidence: 99%

The Glandular Tissue in the Testis of Salamandra salamandra (L.) (Amphibia, Urodela)

Bergmann¹,

Schindelmeiser²,

Greven³

1983

Acta Zoologica

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The glandular tissue in the testis of the urodele Salamandra salamandra was studied by electron microscopy during different developmental stages. After spermiation, steroid hormone (SH)‐secreting cells, which surround seminiferous units (lobules) of the mature part of the testis, proliferate, whereas fibrocytes of the same lobule boundary transform into smooth muscle cells, which in part become closely associated with the epithelium of ductuli efferentes. Sertoli cells within the lobules appear not to be involved in SH‐secretion; they phagocytize spermatozoa which have not been discharged. In the residual stage SH‐secreting cells, as well as Sertoli cells, are degenerated. The tissue is composed of undifferentiated fibrocytic cells, probably derived from smooth muscle cells and/or SH‐secreting cells, of solid cell cords representing the formerly open ductuli efferentes and of primordial germ cells ensheathed by follicle cells. There are indications that smooth muscle cells are involved in phagocytosis when glandular tissue undergoes regression.

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The cycle of foilicuiar and interstitial cells (Leydig cells) in the testis of the marbled newt, Triturus marmoratus (caudata, salamandridae)

Fraile

Sáez

Paniagua

1990

Journal of Morphology

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Ultrastructural examination of the marbled newt (Triturus marmoratus) testis throughout the annual cycle revealed that during the period of testicular quiescence (November-February), primordial germ cells proliferate within cords of filament-rich epithelial cells that will become follicular cells (FCs). Fibroblast-like cells surround the FCs and form the lobule-boundary interstitial cells (ICs). During the period of germ cell development from primordial germ cells to round spermatids (March-June), the FCs surrounding the developing germ cells contain scanty cytoplasm with abundant rough endoplasmic reticulum and scarce filaments. With spermatid elongation (July-August), the FC size grows, its nucleus becomes irregularly outlined, and its cytoplasm displays abundant smooth endoplasmic reticulum, residual bodies, lipid droplets, and large vacuoles. After spermatozoon release by the FCs (August-September), the adjacent ICs increase their size and transform into Leydig cells with abundant smooth endoplasmic reticulum, mitochondria with tubular cristae, and lipid droplets. During the period of testicular quiescence (November-February), the Leydig cells undergo involution, eventually developing the morphological attributes of mesenchymal cells. Intermingled among these cells, cords of filament-rich cells are observed. During this period of the cycle, spermatozoon cysts supported by FCs are present. At the beginning of the germ cell proliferation period (March), these spermatozoa are released, and the adjacent ICs undergo a transformation into Leydig cells similar to those observed in August-September. Maturation and involution of ICs occur when testosterone levels are known to be rising and falling, respectively.

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Seasonal Changes in the Newt, Trituroides hongkongensis, Testis. II. An Ultrastructural Study on the Lobule Boundary Cell

Cited by 10 publications

References 11 publications

HISTOCHEMICAL AND ELECTRON MICROSCOPIC OBSERVATIONS ON THE STEROID HORMONE‐SECRETING CELLS IN THE TESTIS OF THE JAPANESE RED‐BELLIED NEWT, CYNOPS PYRRHOGASTER PYRRHOGASTER

HISTOCHEMICAL AND ELECTRON MICROSCOPIC OBSERVATIONS ON THE STEROID HORMONE‐SECRETING CELLS IN THE TESTIS OF THE JAPANESE RED‐BELLIED NEWT, CYNOPS PYRRHOGASTER PYRRHOGASTER

The Glandular Tissue in the Testis of Salamandra salamandra (L.) (Amphibia, Urodela)

The cycle of foilicuiar and interstitial cells (Leydig cells) in the testis of the marbled newt, Triturus marmoratus (caudata, salamandridae)

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