Seasonal changes of subantarctic benthic bacterial communities

Delille, Daniel

doi:10.1007/bf00008182

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…Indicator and pathogenic bacteria associated with raw or partially treated sewage have been shown to settle into marine sediments, where they accumulate and reach higher densities than in the overlying water column (10,18,24,25). Additionally, lower water temperatures and elevated nutrient concentrations, like those in the immediate area of sewage outfalls, have also been shown to enhance the survival of bacteria in the water column and sediments of southern polar regions (19,20,33,42).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Microbial Indicators and Clostridium perfringens in Wastewater, Water Column Samples, Sediments, Drinking Water, and Weddell Seal Feces Collected at McMurdo Station, Antarctica

Lisle

Smith²,

Edwards

et al. 2004

Appl Environ Microbiol

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McMurdo Station, Antarctica, has discharged untreated sewage into McMurdo Sound for decades. Previous studies delineated the impacted area, which included the drinking water intake, by using total coliform and Clostridium perfringens concentrations. The estimation of risk to humans in contact with the impacted and potable waters may be greater than presumed, as these microbial indicators may not be the most appropriate for this environment. To address these concerns, concentrations of these and additional indicators (fecal coliforms, Escherichia coli, enterococci, coliphage, and enteroviruses) in the untreated wastewater, water column, and sediments of the impacted area and drinking water treatment facility and distribution system at McMurdo Station were determined. Fecal samples from Weddell seals in this area were also collected and analyzed for indicators. All drinking water samples were negative for indicators except for a single total coliform-positive sample. Total coliforms were present in water column samples at higher concentrations than other indicators. Fecal coliform and enterococcus concentrations were similar to each other and greater than those of other indicators in sediment samples closer to the discharge site. C. perfringens concentrations were higher in sediments at greater distances from the discharge site. Seal fecal samples contained concentrations of fecal coliforms, E. coli, enterococci, and C. perfringens similar to those found in untreated sewage. All samples were negative for enteroviruses. A wastewater treatment facility at McMurdo Station has started operation, and these data provide a baseline data set for monitoring the recovery of the impacted area. The contribution of seal feces to indicator concentrations in this area should be considered.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Microbial Indicators and Clostridium perfringens in Wastewater, Water Column Samples, Sediments, Drinking Water, and Weddell Seal Feces Collected at McMurdo Station, Antarctica

Lisle

Smith²,

Edwards

et al. 2004

Appl Environ Microbiol

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“…Predominance of Gram-negative rods in Antarctic marine bacterial communities has been reported widely (Tanner and Herbert, 1982;Bouvy and Delille, 1987;Delille and Cahet, 1985;Delille, 1987;MacCormack and Fraile, 1990). However, unlike pelagic bacterioplanktonic communities, in which non-fermentative Gram-negative rods were always dominant (Delille, 1990) some exceptions were observed in benthic communities (Delille, 1995). The other major taxonomic groups observed were Micrococcus, Vibrio and Bacillus (Fig.…”

Section: Spatial and Temporal Variabilitymentioning

confidence: 94%

“…Bacterial degradation of organic carbon has been described in pelagic habitats (Morita et al, 1971;Brlter and Dawson, 1982;Bird and Karl, 1991;Delille, 1993) and the benthos (Delille and Cahet, 1985;Nedwell et al, 1993;Delille, 1995). However, psychrophilic methanogenic bacteria have yet to be isolated from these habitats (Jarrel and Kalmokoff 1988).…”

Section: Metabolic Diversitymentioning

confidence: 99%

Biodiversity and function of bacteria in the Southern Ocean

Delille

1996

Biodivers Conserv

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“…Pseudomonads are found in many aquatic ecosystems by both culture-dependent (11,12,17) and culture-independent techniques (9,10,16,25,39). Pseudomonads are known as early colonizers of "new" habitats, such as developing root systems and food products, indicating an opportunistic growth strategy in response to available nutrient resources.…”

mentioning

confidence: 99%

Input of Protein to Lake Water Microcosms Affects Expression of Proteolytic Enzymes and the Dynamics ofPseudomonasspp

Worm¹,

Nybroe²

2001

Appl Environ Microbiol

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The objective of this study was to determine how an input of protein to lake water affects expression of a proteolytic potential and influences the abundance and composition of a specific group of bacteria. Pseudomonas spp. were chosen as a target group that can be recovered on selective growth media and contain both proteolytic and nonproteolytic strains. Amendment with 2 mg of casein per liter increased total proteinase activity (hydrolysis of [ 3 H]casein) by 74%, leucine-aminopeptidase activity (hydrolysis of leucine-methylcoumarinylamide) by 133%, bacterial abundance by 44%, and phytoplankton biomass (chlorophyll a) by 39%. The casein amendment also increased the abundance of culturable Pseudomonas spp. by fivefold relative to control microcosms but did not select for proteolytic isolates. Soluble proteins immunochemically related to the Pseudomonas fluorescens alkaline proteinase, AprX, were detected in amended microcosms but not in the controls. The expression of this class of proteinase was confirmed exclusively for proteolytic Pseudomonas isolates from the microcosms. The population structure of Pseudomonas isolates was determined from genomic fingerprints generated by universally primed PCR, and the analysis indicated that casein amendment led to only minor shifts in population structure. The appearance of AprX-like proteinases in the lake water might thus reflect a general induction of enzyme expression rather than pronounced shifts in the Pseudomonas population structure. The limited effect of casein amendment on Pseudomonas population structure might be due to the availability of casein hydrolysates to bacteria independent of their proteinase expression. In the lake water, 44% of the total proteinase activity was recovered in 0.22-m-pore-size filtrates and thus without a direct association with the bacteria providing the extracellular enzyme activity. Since all Pseudomonas isolates expressed leucine-aminopeptidase in pure culture, proteolytic as well as nonproteolytic pseudomonads were likely members of the bacterial consortium that metabolized protein in the lake water.

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Seasonal changes of subantarctic benthic bacterial communities

Cited by 14 publications

References 44 publications

Occurrence of Microbial Indicators and Clostridium perfringens in Wastewater, Water Column Samples, Sediments, Drinking Water, and Weddell Seal Feces Collected at McMurdo Station, Antarctica

Occurrence of Microbial Indicators and Clostridium perfringens in Wastewater, Water Column Samples, Sediments, Drinking Water, and Weddell Seal Feces Collected at McMurdo Station, Antarctica

Biodiversity and function of bacteria in the Southern Ocean

Input of Protein to Lake Water Microcosms Affects Expression of Proteolytic Enzymes and the Dynamics ofPseudomonasspp

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