Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event

Mokrina, Mariia; Nagasawa, Kazue; Kanamori, Makoto; Natsuike, Masafumi; Osada, Makoto

doi:10.1016/j.aqrep.2021.100613

Cited by 5 publications

(1 citation statement)

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“…An antibody against AXDND1 was prepared using a recombinant protein as outlined previously. 20 The partial cDNA sequence of AXDND1(CCDS83611.1) corresponding to 958–1111 aa was amplified by PCR, inserted into the His‐tagged pCold‐pros2 vector (Takara Bio), and transformed into DH5a competent E. coli (Takara Bio). The selected clone was further transformed into the BL21 (DE3) E. coli strain (Takara Bio), and protein expression was induced with IPTG.…”

Section: Methodsmentioning

confidence: 99%

Loss of Axdnd1 causes sterility due to impaired spermatid differentiation in mice

Hiradate

Harima

Yanai

et al. 2022

Reprod Medicine & Biology

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Purpose Spermiogenesis, the process of deformation of sperm head morphology and flagella formation, is a phenomenon unique to sperm. Axonemal dynein light chain proteins are localized to sperm flagella and are known to be involved in sperm motility. Here, we focused on the gene axonemal dynein light chain domain containing 1 ( Axdnd1 ) with the aim to determine the function of its protein product AXDND1. Methods To elucidate the role of AXDND1 in spermatogenesis, we generated Axdnd1 knockout (KO) mice using the CRISPR/Cas9 system. The generated mice were subjected to fertility tests and analyzed by immunohistochemistry. Result The Axdnd1 KO mouse exhibited sterility caused by impaired spermiogenesis during the elongation step as well as abnormal nuclear shaping and manchette, which are essential for spermiogenesis. Moreover, AXDND1 showed enriched testicular expression and was localized from the mid‐pachytene spermatocytes to the early spermatids. Conclusion Axdnd1 is essential for spermatogenesis in the mouse testes. These findings improve our understanding of spermiogenesis and related defects. According to a recent report, deleterious heterozygous mutations in AXDND1 were found in non‐obstructive azoospermia (NOA) patients. Therefore, Axdnd1 KO mice could be used as a model system for NOA, which will greatly contribute to future NOA treatment studies.

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Section: Methodsmentioning

confidence: 99%