Mobility problems in large cities of Ukraine and Eastern Europe are complicated by the fact that the increase of private transport volume significantly exceeds street and road network`s capacity. This is most noticeable during peak periods in terms of daylight hours and throughout the year. From the point of sustainable mobility view, this negative phenomenon significantly affects urban public transport, which does not have separate dedicated traffic lines. This article analyzes the issue regarding the deterioration of the transport situation in large cities. The reason for this is the increase in traffic on main streets during the day peaks, as well as the presence of seasonal traffic factors. If the issue of the occurrence and traffic jams duration and the increase in the correspondence time of private transport is sufficiently studied, then the problems of changing the schedules of public transport and taking into account the increase in the trip duration depending on the time of year need to be clarified. The routes of public transport, which do not have a separate infrastructure and move in the general flow together with private cars, were chosen for the study. According to the results of remote monitoring of public transport, a change in the trip duration and time lost due to the boarding and disembarking of passengers on similar trolleybus routes in different seasons was established. Based on the obtained data, a matrix of trip duration unevenness coefficients for public transport routes was formed, and a measure of the seasonality effect on these indicators was established. The obtained results make it possible to quantitatively determine the influence of the season and time of the day on the change in the trip duration, which can be applied in further studies using simulation tools and for practical use in drawing up seasonal traffic schedules. The results of the research complement the currently relevant scientific works, which concern the problems of seasonal mobility, as well as the influence of the social infrastructure objects functioning (schools, kindergartens, and other educational institutions) of cities on the peak load of the street and road network, which extends the duration of traffic not only for private but also public transport.