Seasonal distribution and prevalence of diarrheagenic Escherichia coli in different aquatic environments in Taiwan

Huang, Wen‐Chien; Hsu, Bing-Mu; Kao, Po-Min; Tao, Chi‐Wei; Ho, Ying-Ning; Kuo, Chun‐Wei; Huang, Yatao

doi:10.1016/j.ecoenv.2015.09.040

Cited by 19 publications

(12 citation statements)

References 29 publications

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“…In recent years, although substantial research has been carried out in other countries, such as China, India, Poland, and Brazil, regarding MDR bacteria and the development of resistance mechanisms in aquatic systems (Diwan et al, 2010;Korzeniewska and Harnisz, 2013;Rebello and Regua-Mangia, 2014;Xu et al, 2016), there are few studies available concerning the prevalence of MDR enteropathogenic bacteria in aquatic environments in Malaysia. This is despite the fact that the microbiological qualities of river water sources in other countries have been reported (Hamelin et al, 2007;Huang et al, 2016;Olaniran et al, 2009;Ramírez Castillo et al, 2013).…”

Section: Introductionmentioning

confidence: 91%

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Hara

Yusaimi

Zulkeflle

et al. 2018

J. Gen. Appl. Microbiol.

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The emergence of antibiotic resistance among multidrug-resistant (MDR) microbes is of growing concern, and threatens public health globally. A total of 129 Escherichia coli isolates were recovered from lowland aqueous environments near hospitals and medical service centers in the vicinity of Kuala Lumpur, Malaysia. Among the eleven antibacterial agents tested, the isolates were highly resistant to trimethoprim-sulfamethoxazole (83.7%) and nalidixic acid (71.3%) and moderately resistant to ampicillin and chloramphenicol (66.7%), tetracycline (65.1%), fosfomycin (57.4%), cefotaxime (57.4%), and ciprofloxacin (57.4%), while low resistance levels were found with aminoglycosides (kanamycin, 22.5%; gentamicin, 21.7%). The presence of relevant resistance determinants was evaluated, and the genotypic resistance determinants were as follows: sulfonamides (sulI, sulII, and sulIII), trimethoprim (dfrA1 and dfrA5), quinolones (qnrS), β-lactams (ampC and blaCTX-M), chloramphenicol (cmlA1 and cat2), tetracycline (tetA and tetM), fosfomycin (fosA and fosA3), and aminoglycosides (aphA1 and aacC2). Our data suggest that multidrug-resistant E. coli strains are ubiquitous in the aquatic systems of tropical countries and indicate that hospital wastewater may contribute to this phenomenon.

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Section: Introductionmentioning

confidence: 91%

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Hara

Yusaimi

Zulkeflle

et al. 2018

J. Gen. Appl. Microbiol.

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“…Although the diversity and dynamics of microbes in karst springs (Farnleitner et al., ; Ohad et al., ; Savio et al., ), unsaturated and saturated karst aquifers (Cooper et al., ; Gray & Engel, ; Johnson et al., ; Lin et al., ; Menning et al., ), and water pools (Shabarova et al., ) as well as in groundwater‐surface water exchange systems (Li, Song, et al., ) have been discussed in the literature, much less attention has been paid to the structure of bacterioplankton communities in dammed karst rivers. In addition, previous studies on bacterioplankton communities in the canyon‐shaped and meso‐eutrophic Rimov Reservoir (Simek et al., ), the dammed Ebro River (Ruiz‐González, Proia, Ferrera, Gasol, & Sabater, ), and the rivers controlled by the Three Gorges Dam (Huang et al., ; Li, Lu, et al., ; Yan et al., ) did not include the seasonal variation or depth dynamics in bacterioplankton.…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal dynamics of bacterioplankton community composition in a subtropical dammed karst river of southwestern China

Shi

Song

et al. 2019

MicrobiologyOpen

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River damming influences the hydro‐physicochemical variations in karst water; however, such disruption in bacterioplankton communities has seldom been studied. Here, three sampling sites (city‐river section, reservoir area, and outflow area) of the Ca2+–Mg2+–HCO3−–SO42− water type in the dammed Liu River were selected to investigate the bacterioplankton community composition as identified by high‐throughput 16S rRNA gene sequencing. In the dammed Liu River, thermal regimes have been altered, which has resulted in considerable spatial‐temporal differences in total dissolved solids (TDSs), oxidation‐reduction potential (Eh), dissolved oxygen (DO), and pH and in a different microenvironment for bacterioplankton. Among the dominant bacterioplankton phyla, Proteobacteria, Actinobacteria, Bacteroidetes, and Cyanobacteria account for 38.99%–87.24%, 3.75%–36.55%, 4.77%–38.90%, and 0%–14.44% of the total reads (mean relative frequency), respectively. Bacterioplankton communities are dominated by Brevundimonas, Novosphingobium, Zymomonas, the Actinobacteria hgcIclade, the CL500‐29 marine group, Sediminibacterium, Flavobacterium, Pseudarcicella, Cloacibacterium, and Prochlorococcus. Their abundances covary with spatial‐temporal variations in hydro‐physicochemical factors, as also demonstrated by beta diversity analyses. In addition, temperature plays a pivotal role in maintaining bacterioplankton biodiversity and hydro‐physicochemical variations. This result also highlights the concept that ecological niches for aquatic bacteria in dammed karst rivers do not accidentally occur but are the result of a suite of environmental forces. In addition, bacterioplankton can alter the aquatic carbon/nitrogen cycle and contribute to karst river metabolism.

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“…The aquatic bacteria with high drug-resistant profiles were mostly found to be Enterobacteriaceae (Especially in E. coli , Salmonella , etc. ), Enterococcaceae, and Staphylococcaceae, among others [ 34 , 35 , 36 , 49 , 50 , 51 ]. Furthermore, most V. vulnificus populations with high drug-resistance were isolated from the shellfish [ 27 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence, Genetic Diversity, Antimicrobial Resistance, and Toxigenic Profile of Vibrio vulnificus Isolated from Aquatic Environments in Taiwan

et al. 2021

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Vibrio vulnificus is a gram-negative, opportunistic human pathogen associated with life-threatening wound infections and is commonly found in warm coastal marine water environments, globally. In this study, two fishing harbors and three tributaries of the river basin were analyzed for the prevalence of V. vulnificus in the water bodies and shellfish that are under the pressure of external pollutions. The average detection rate of V. vulnificus in the river basins and fishing harbors was 8.3% and 4.2%, respectively, in all seasons. A total of nine strains of V. vulnificus were isolated in pure cultures from 160 samples belonging to river basins and fishing harbors to analyze the antibiotic susceptibility, virulence gene profiles, and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) fingerprinting. All isolates were susceptible to 10 tested antibiotics. The genotypic characterization revealed that 11.1% (n = 1/9) strain was nonvirulent, whereas 88.9% (n = 8/9) isolates were virulent strains, which possessed the four most prevalent toxin genes such as vcgC (88.9%), 16S B (88.9%), vvhA (88.9%), and manIIA (88.9%), followed by nanA (77.8%), CPS1 (66.7), and PRXII (44.4%). Additionally, ERIC-PCR fingerprinting grouped these nine isolates into two main clusters, among which the river basin isolates showed genetically diverse profiles, suggesting multiple sources of V. vulnificus. Ultimately, this study highlighted the virulent strains of V. vulnificus in the coastal aquatic environments of Taiwan, harboring a potential risk of infection to human health through water-borne transmission.

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Seasonal distribution and prevalence of diarrheagenic Escherichia coli in different aquatic environments in Taiwan

Cited by 19 publications

References 29 publications

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Spatial and temporal dynamics of bacterioplankton community composition in a subtropical dammed karst river of southwestern China

Prevalence, Genetic Diversity, Antimicrobial Resistance, and Toxigenic Profile of Vibrio vulnificus Isolated from Aquatic Environments in Taiwan

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