Seasonal Distribution, Blood-Feeding Habits, and Viruses of Mosquitoes in an Open-Faced Quarry in Connecticut, 2010 and 2011

Anderson, John F.; Armstrong, Philip M.; Misencik, Michael J.; Bransfield, Angela B.; Andreadis, Theodore G.; Molaei, Goudarz

doi:10.2987/17-6707.1

Cited by 16 publications

(20 citation statements)

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“…However, other studies have shown mammals other than humans to be the primary source of blood meal. In Maryland, 50% of blood meals originated from rats [ 29 ], and multiple studies have shown that most blood meals (53–100%) were derived from white-tailed deer [ 36 – 39 ]. The frequency of white-tailed deer as hosts for Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Multiple studies in the northeastern United States have found that white-tailed deer, the most abundant large mammal in the region, represent the majority of blood meals identified from Ae. japonicus [ 36 – 39 ]. But other mammalian hosts have also been identified including the domestic cat [ 29 , 40 ], brown rat [ 29 ], opossum [ 38 ], cow [ 41 ], chipmunk [ 37 ], and horse [ 36 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

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Spatiotemporal distribution, abundance, and host interactions of two invasive vectors of arboviruses, Aedes albopictus and Aedes japonicus, in Pennsylvania, USA

et al. 2022

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Background Aedes albopictus and Aedes japonicus, two invasive mosquito species in the United States, are implicated in the transmission of arboviruses. Studies have shown interactions of these two mosquito species with a variety of vertebrate hosts; however, regional differences exist and may influence their contribution to arbovirus transmission. Methods We investigated the distribution, abundance, host interactions, and West Nile virus infection prevalence of Ae. albopictus and Ae. japonicus by examining Pennsylvania mosquito and arbovirus surveillance data for the period between 2010 and 2018. Mosquitoes were primarily collected using gravid traps and BG-Sentinel traps, and sources of blood meals were determined by analyzing mitochondrial cytochrome b gene sequences amplified in PCR assays. Results A total of 10,878,727 female mosquitoes representing 51 species were collected in Pennsylvania over the 9-year study period, with Ae. albopictus and Ae. japonicus representing 4.06% and 3.02% of all collected mosquitoes, respectively. Aedes albopictus was distributed in 39 counties and Ae. japonicus in all 67 counties, and the abundance of these species increased between 2010 and 2018. Models suggested an increase in the spatial extent of Ae. albopictus during the study period, while that of Ae. japonicus remained unchanged. We found a differential association between the abundance of the two mosquito species and environmental conditions, percent development, and median household income. Of 110 Ae. albopictus and 97 Ae. japonicus blood meals successfully identified to species level, 98% and 100% were derived from mammalian hosts, respectively. Among 12 mammalian species, domestic cats, humans, and white-tailed deer served as the most frequent hosts for the two mosquito species. A limited number of Ae. albopictus acquired blood meals from avian hosts solely or in mixed blood meals. West Nile virus was detected in 31 pools (n = 3582 total number of pools) of Ae. albopictus and 12 pools (n = 977 total pools) of Ae. japonicus. Conclusions Extensive distribution, high abundance, and frequent interactions with mammalian hosts suggest potential involvement of Ae. albopictus and Ae. japonicus in the transmission of human arboviruses including Cache Valley, Jamestown Canyon, La Crosse, dengue, chikungunya, and Zika should any of these viruses become prevalent in Pennsylvania. Limited interaction with avian hosts suggests that Ae. albopictus might occasionally be involved in transmission of arboviruses such as West Nile in the region. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatiotemporal distribution, abundance, and host interactions of two invasive vectors of arboviruses, Aedes albopictus and Aedes japonicus, in Pennsylvania, USA

et al. 2022

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“…Aedes canadensis is distributed throughout Canada as well as the Northern, Eastern, and Midwestern states in the U.S.A ( 29 ). This species does take blood meals from deer ( 28 , 30 – 33 ). Jamestown Canyon virus was detected in 2 studies from field-collected adult female Ae .…”

Section: Discussionmentioning

confidence: 99%

Outbreak Investigation: Jamestown Canyon Virus Surveillance in Field-Collected Mosquitoes (Diptera: Culicidae) From Wisconsin, USA, 2018–2019

Farquhar

Thrun

Tucker

2022

Front. Public Health

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During the summers of 2017–2019, 60 human cases of Jamestown Canyon virus-associated disease were reported in the State of Wisconsin, U.S.A; by comparison, there were 28 cases in the 5 years prior. Jamestown Canyon virus (JCV, Peribunyaviridae: Orthobunyavirus) is a zoonotic, mosquito-borne virus that is endemic throughout North America. The proposed transmission cycle for JCV involves horizontal transmission by a variety of mammal-feeding mosquito species and deer hosts, and transseasonal maintenance by vertical transmission in Aedes mosquito species. Although some of the earliest work on JCV transmission and disease was done in Wisconsin (WI), little is known about the spectrum of mosquitoes that are currently involved in transmission and maintenance of JCV, which is key to inform the approach to control and prevent JCV transmission, and to understand why case numbers have increased dramatically in recent years. Therefore, we undertook an intensive surveillance effort in Sawyer and Washburn counties, WI between April and August of 2018 and 2019, in an area with a concentration of JCV human cases. Larval and adult stages of mosquitoes were surveyed using larval dippers and emergence traps, light traps, resting boxes, a Shannon-style trap, and backpack aspirator. In total, 14,949 mosquitoes were collected in 2018, and 28,056 in 2019; these specimens represent 26 species in 7 genera. Suspect vector species were tested for JCV by polymerase chain reaction (PCR); of 23 species that were tested, only Aedes provocans yielded JCV positive results. In 2018, a single pool of Ae. provocans tested positive. In 2019, with more focused early season surveillance, we detected JCV in 4 pools of adult mosquitoes, and one pool that consisted of lab-raised adults that were collected as larvae. Material from all of these PCR-positive samples also yielded infectious virus in cell culture. Overall, these data provide new insight into the seasonality and habitat preferences for 26 mosquito species in Northern WI, which will be useful to inform future surveillance efforts for JCV. The results underscore the importance of Ae. provocans as a vector species involved in transseasonal maintenance of JCV in this region.

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“…The ability of An. punctipennis to transmit arboviruses such as Cache Valley [12] and eastern equine encephalomyelitis [13] viruses, as well as a nematode Diro llaria immitis [14] has also been reported.…”

Section: Introductionmentioning

confidence: 99%

The New Internal Transcribed Spacer 2 Diagnostic Tool Clarifies the Taxonomic Position and Geographic Distribution of the North American Malaria Vector Anopheles Punctipennis

Hodge

Yurchenko

Karagodin

et al. 2020

Preprint

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Background: The malaria mosquito Anopheles punctipennis Say, a widely distributed species in North America, is capable of transmitting human malaria and is actively involved in the transmission of the ungulate malaria parasite Plasmodium odocoilei. However, molecular diagnostic tools based on Internal Transcribed Spacer 2 (ITS2) of ribosomal DNA are lacking for An. punctipennis. An. punctipennis is a former member of the Anopheles maculipennis complex but its systematic position remains unclear. Results: In this study, we collected and sequenced ITS2 from 276 An. punctipennis specimens collected from the eastern and midwestern United States. Our analysis demonstrated a consistent sequence length and showed no indications of intragenomic variation among the samples based on ITS2, suggesting that An. punctipennis represents a single species in the studied geographic locations. In addition to the ITS2 sequence-based molecular tool, we developed a simple and robust Restriction Length Polymorphism approach for species identification. An. punctipennis was found in urban, rural, and forest settings, suggesting its potential broad role in pathogen transmission. Phylogeny based on ITS2 sequence comparison demonstrated the close relationship of this species with other members of the Maculipennis group. Conclusions: This study developed molecular tools based on ITS2 sequences for the malaria vector An. punctipennis and clarified the phylogenetic position of the species within the Maculipennis group.

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Seasonal Distribution, Blood-Feeding Habits, and Viruses of Mosquitoes in an Open-Faced Quarry in Connecticut, 2010 and 2011

Cited by 16 publications

References 28 publications

Spatiotemporal distribution, abundance, and host interactions of two invasive vectors of arboviruses, Aedes albopictus and Aedes japonicus, in Pennsylvania, USA

Spatiotemporal distribution, abundance, and host interactions of two invasive vectors of arboviruses, Aedes albopictus and Aedes japonicus, in Pennsylvania, USA

Outbreak Investigation: Jamestown Canyon Virus Surveillance in Field-Collected Mosquitoes (Diptera: Culicidae) From Wisconsin, USA, 2018–2019

The New Internal Transcribed Spacer 2 Diagnostic Tool Clarifies the Taxonomic Position and Geographic Distribution of the North American Malaria Vector Anopheles Punctipennis

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