Seasonal Distribution of Some Arthropod Parasites of Rabbits in Louisiana1

Farlow, Joseph E.; Burns, Edward C.; Newsom, John D.

doi:10.1093/jmedent/6.2.172

Cited by 4 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these environments, opportunities for virus transmission to humans are limited. On the other hand, cottontail rabbits and the ticks commonly infesting them (1,8,22,24,25) are widely distributed in North America, thus providing more opportunities for virus transmission to other hosts. Two of these ticks, Haemaphysalis leporispalustris and Ixodes dentatus, rarely bite humans (1), but a third, Amblyomma americanum, does and is prevalent throughout the southeastern United States.…”

mentioning

confidence: 99%

Rabbit Syncytium Virus Is a Kemerovo Serogroup Orbivirus

Theil

McCloskey

1991

J Clin Microbiol

View full text Add to dashboard Cite

Polyacrylamide gel genome electropherotyping and negative-stain electron microscopic studies, along with immunofluorescent staining and immune electron microscopy reactions, indicate that rabbit syncytium virus has the morphologic, genomic, and antigenic attributes of a Kemerovo serogroup orbivirus. Rotaviruses cause diarrhea in humans and other animals (7), and at least five distinct rotavirus serogroups, designated A through E, exist (18, 19). The discovery that trypsin permits group A rotavirus cell culture propagation (26) has allowed extensive characterization of isolates belonging to this serogroup. However, techniques for routine serial propagation of non-group A rotaviruses in cell culture are lacking, and investigations on these viruses are seriously restricted. Consequently, cultivatable non-group A rotaviruses are of considerable medical and veterinary importance. Rabbit syncytium virus, isolated from a healthy cottontail rabbit (Sylvilagus floridanus) trapped in Quinby, Va., in 1962, induces syncytia in some cell cultures (16). This virus most closely resembled the EDIM agent (a group A rotavirus) in morphology and morphogenesis, but was antigenically unrelated to it (4, 16). Syncytial cell formation, however, often occurs during group B rotaviral infections in vivo and in vitro (30, 32, 33). Therefore, we investigated the possibility that rabbit syncytium virus might be a cultivatable group B rotavirus. Amniotic-allantoic fluid from the 12th egg passage of rabbit syncytium virus (10 January, 1963) recovered from rabbit 32 and the 5th egg passage of its reisolation from rabbit 32 tissues (29 August, 1964) was kindly provided by M. F. Bozeman, Food and Drug Administration, Department of Health & Human Services, Bethesda, Md. Roller tube monolayers of MA104 and BHK-21 cells, prepared as described previously (28) but without trypsin in the maintenance medium, were inoculated with egg-passaged virus diluted 10-fold. Rabbit syncytium virus was recovered from each egg passage and was further passaged in both cells as described above, although only BHK-21 cells were used for the later passages. Virus passages were stored at 4 or-20°C in 50% glycerol. Rabbit syncytium virus induced a cytopathic effect in both cell lines which was characterized by foci of rounded cells that gradually enlarged to destroy the monolayer. Some MA104 cell monolayers, prepared on coverslips as described previously (30), were infected with rabbit syncytium virus and subsequently stained with Harris hematoxylin and eosin after fixation in Bouin solution. Stained infected monolayers contained many cells with eosinophilic and basophilic cytoplasmic inclusion bodies, but no syncytia (Fig. 1). Other infected MA104 cell coverslip monolayers were examined by immunofluorescence microscopy, using fluorescein-conju-* Corresponding author. t Ohio State University Journal Article no. 3291.

show abstract

mentioning

confidence: 99%