Seasonal distribution of Vibrio parahaemolyticus in freshwater environs and in association with freshwater fishes in Calcutta

Abstract

The seasonal distribution of Vibrio parahaemolyticus in freshwater environs and in association with freshwater fishes was studied in 1982 and 1983. The occurrence of this organism in water and sediments at the three sites studied was very infrequent and was restricted to the summer months, although it was not always isolated during these months. The association of V. parahaemolyticus with plankton was chiefly confined to the summer months and progressively declined with the onset of monsoons, remaining below detectable levels during the postmonsoon and winter months. The incidence and counts of V. parahaemolyticus were consistently higher in association with plankton than with water and sediment samples. V. parahaemolyticus could be recovered throughout the period of investigation from freshly caught and market samples of freshwater fishes. The highest recovery rate of this halophile from fishes was invariably from fecal samples. Most of the strains isolated ih this study were untypable, and those which could be typed were predominantly serotypes encountered in the environment. All the isolates were Kanagawa negative. From this study, it could be concluded that the survival of V. parahaemolyticus in freshwater ecosystems is transient and dependent on a biological host.

Genetic and virulence characterisation of Vibrio parahaemolyticus isolated from Indian coast

“…An abundance of V. parahaemolyticus was seen in the water compared to plankton. This was in contrast to other studies where higher densities of V. parahaemolyticus were obtained from plankton [26,27]. However, higher numbers of the bacterium were found in the Georgian coastal waters [28].…”

Seasonal distribution of facultatively enteropathogenic vibrios (Vibrio cholerae, Vibrio mimicus, Vibrio parahaemolyticus) in the freshwater of the Elbe River at Hamburg

“…Several workers have identified the organism in European coastal waters (Hechelmann et al 1971 (Heinemeyer 1984). The occurrence of these halophilic organisms in freshwater environments, however, is exceptional and it has hitherto been reported from only Calcutta (Sarkar et al 1983(Sarkar et al , 1985.…”

“…Studies of other workers have shown that water and sediments offer a rather limited ecological niche for V . parahuemolyticus (Kaneko & Colwell 1973;Sarkar et a/. 1985).…”

Enumeration of Vibrio parahaemolyticus in oyster tissues following artificial contamination and depuration

“…Green fluorescent protein (GFP) tracing has been utilized to indirectly locate V. parahaemolyticus in the gills and other tissues (Cabello et al 2005). Although V. parahaemolyticus has been detected in the gills (Sarkar et al 1985;Cabello et al 2005;Luan et al 2008) and in individual oysters (Parveen et al 2008), the actual levels of the pathogen in specific oyster tissues are not yet clear. Therefore, this study investigated the abundance of V. parahaemolyticus (ATCC 17802) in various oyster tissues following artificial contamination and depuration, to determine which tissues may be suitable for direct monitoring of this pathogen.…”