Seasonal Dynamics of Bacteria in Corrosive Biofilms Formed on the Surface of Wastewater Treatment Plants

Abdulina, D. R.; Kurmakova, I. N.; Bondar, E. S.; Демченко, Н. Р.; Bondarenko, A. I.

doi:10.3103/s1063455x19010077

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…On the contrary, more biofilm was formed by bacterial isolates from the sedimentation tank from summer-period compared to winter-period in TSB (Figure 3). Abdulina et al (2019) showed that active biofilm is formed in wastewater treatment plants regardless of the season [57]. Bacterial isolates from the nitrification tank were better biofilm formers compared to bacterial isolates from the sedimentation tank in TSB (Figures 2 and 3).…”

Section: Crystal Violet Assay For Determination Of Biofilm Formationmentioning

confidence: 94%

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

et al. 2020

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The study monitored the effect of tetracycline on bacterial biofilm formation and compared biofilm formation by resistant bacterial strains in different phases of the wastewater treatment process in wastewater treatment plant (WWTP). The crystal violet staining method was used to evaluate the biofilm formation. Biofilm-related bacterial properties were characterized by hydrophobicity, autoaggregation and motility tests. The relative abundance of tetracycline resistance genes (tetW, tetM, tetO, tetA and tetB) in wastewaters were subsequently quantified using qPCR. The results show that the isolates from the nitrification tank produce biofilm with up to 10 times greater intensity relative to the isolates from the sedimentation tank. In isolates of Aeromonas sp. from the nitrification tank, increased biofilm production in the occurrence of tetracycline from a concentration of 0.03125 µg/mL was observed. The tetW gene showed the highest relative abundance out of all the tested genes. From the sampling points, its abundance was the highest in the sedimentation tank of the WWTP. Based on these results, it can be assumed that resistant bacteria are able to form a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation. WWTPs thus represent a reservoir of antibiotic resistance genes and contribute to the spread of resistance in the natural environment.

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Section: Crystal Violet Assay For Determination Of Biofilm Formationmentioning

confidence: 94%

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

et al. 2020

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“…The calibration curve has been constructed with the same process. Calibration solutions have been prepared in distilled water at concentrations of 2,4,8,16,24,32,40, and 48 µmol L −1 of sodium sulfate [43].…”

Section: Sulfate Determinationmentioning

confidence: 99%

“…They can be found in a number of natural ecotopes and in human-caused and industrial systems, such as sea sludge deposits, hydrothermal springs, freshwaters, soils, anaerobic muds, as well as wastewaters, oil and gas fields, on the surfaces of metal constructions of gas and oil communications, in oil tankers, thermal systems, sewers, reinforced concrete and metal installations, etc. [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. SRB could live in animal and human intestines [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Adenosine-5′-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

et al. 2020

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A comparative study of the kinetic characteristics (specific activity, initial and maximum rate, and affinity for substrates) of key enzymes of assimilatory sulfate reduction (APS reductase and dissimilatory sulfite reductase) in cell-free extracts of sulphate-reducing bacteria (SRB) from various biotopes was performed. The material for the study represented different strains of SRB from various ecotopes. Microbiological (isolation and cultivation), biochemical (free cell extract preparation) and chemical (enzyme activity determination) methods served in defining kinetic characteristics of SRB enzymes. The determined affinity data for substrates (i.e., sulfite) were 10 times higher for SRB strains isolated from environmental (soil) ecotopes than for strains from the human intestine. The maximum rate of APS reductase reached 0.282–0.862 µmol/min×mg−1 of protein that is only 10 to 28% higher than similar initial values. The maximum rate of sulfite reductase for corrosive relevant collection strains and SRB strains isolated from heating systems were increased by 3 to 10 times. A completely different picture was found for the intestinal SRB Vmax in the strains Desulfovibrio piger Vib-7 (0.67 µmol/min × mg−1 protein) and Desulfomicrobium orale Rod-9 (0.45 µmol/min × mg−1 protein). The determinant in the cluster distribution of SRB strains is the activity of the terminal enzyme of dissimilatory sulfate reduction—sulfite reductase, but not APS reductase. The data obtained from the activity of sulfate reduction enzymes indicated the adaptive plasticity of SRB strains that is manifested in the change in enzymatic activity.

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Seasonal Dynamics of Bacteria in Corrosive Biofilms Formed on the Surface of Wastewater Treatment Plants

Cited by 2 publications

References 5 publications

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

Adenosine-5′-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

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