Seasonal Dynamics of Gastrointestinal Nematode Infections of Goats and Emergence of Ivermectin Resistance in Haemonchus contortus in Hubei Province, China

Yuan, Wei; Ke, Lu; Li, Hao; He, Chuanchuan; Feng, Jintao; Zhang, Xin; Mao, Yudan; Hong, Yang; Zhou, Yu; Lu, Jun; Jin, Yamei; Lin, Jiaojiao

doi:10.2478/s11686-019-00067-3

Cited by 11 publications

(11 citation statements)

References 9 publications

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“…As H. contortus has shown a strong ability to develop resistance, IVM resistance in this parasite from different continents has been extensively studied and well documented [ 26 – 32 ]. However, our understanding of the molecular basis of the resistance mechanism is patchy.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus

Tuersong

Zhou

et al. 2022

Parasites Vectors

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Background Ivermectin (IVM) is one of the most important and widely used anthelmintics in veterinary medicine. However, its efficacy is increasingly compromised by widespread resistance, and the exact mechanism of IVM resistance remains unclear for most parasitic nematodes, including Haemonchus contortus, a blood-sucking parasitic nematode of small ruminants. Methods In this study, an H. contortus IVM-resistant strain from Zhaosu, Xinjiang, China, was isolated and assessed by the control test, faecal egg count reduction test (FECRT) and the larval development assay (LDA). Subsequently, comparative analyses on the transcriptomics of IVM-susceptible and IVM-resistant adult worms of this parasite were carried out using RNA sequencing (RNA-seq) and bioinformatics. Results In total, 543 (416 known, 127 novel) and 359 (309 known, 50 novel) differentially expressed genes (DEGs) were identified in male and female adult worms of the resistant strain compared with those of the susceptible strain, respectively. In addition to several previously known candidate genes which were supposed to be associated with IVM resistance and whose functions were involved in receptor activity, transport, and detoxification, we found some new potential target genes, including those related to lipid metabolism, structural constituent of cuticle, and important pathways such as antigen processing and presentation, lysosome, autophagy, apoptosis, and NOD1-like receptor signalling pathways. Finally, the results of quantitative real-time polymerase chain reaction confirmed that the transcriptional profiles of selected DEGs (male: 8 genes, female: 10 genes) were consistent with those obtained by the RNA-seq. Conclusions Our results indicate that IVM has multiple effects, including both neuromuscular and non-neuromuscular targets, and provide valuable information for further studies on the IVM resistance mechanism in H. contortus. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus

Tuersong

Zhou

et al. 2022

Parasites Vectors

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“…As H. contortus has shown a strong ability to develop resistance, IVM resistance in this parasite from different continents has been extensively studied and well documented [25][26][27][28][29][30][31]. However, our understanding of the molecular basis of the resistant mechanism is patchy.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis on Transcriptomics of Ivermectin Resistant and Sensitive Strains of Haemonchus Contortus

Tuersong

Zhou

et al. 2022

Preprint

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Background: Ivermectin (IVM) is one of the most important and widely used anthelmintics in veterinary medicine. However, its efficacy is increasingly compromised by widespread resistance, and the exact mechanism of IVM resistance remains unclear for most parasitic nematodes including Haemonchus contortus, a blood-sucking parasitic nematode of small ruminants.Methods: In this study, we isolated and assessed an IVM resistant strain from Zhaosu, Xinjiang, China. Subsequently, the comparative analyses on transcriptomics of IVM susceptible and resistant H. contortus adult worms were carried out using RNA sequencing and bioinformatics.Results: In total, 543 and 359 differentially expressed genes (DEGs) were identified in male and female adult worms of the resistant strain compared with the susceptible strain, respectively. The DEGs encode molecules involved in receptor activities, transport, detoxification, lipid metabolism and cuticle collagen formation. In addition, Gene Ontology (GO) analysis revealed that transcriptional changes were dominant in genes associated with ligand-gated channel activity, oxidation-reduction process, lipid metabolic process, and structural constituent of cuticle. The results support previous proposal that the IVM resistant mechanism of H. contortus involved in both neuromuscular and non-neuromuscular pathways. Finally, the quantitative RT-PCR results confirmed that the transcriptional profiles of selected DEGs (male: 8 genes, female: 10 genes) were consistent with those obtained by the RNA-Seq.Conclusions: The findings from this work provided valuable information for further studies on the IVM resistance in H. contortus.

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“…As H. contortus has shown a strong ability to develop resistance, IVM resistance in this parasite is extensively studied and well documented [17]. Today, most of the eld isolates of H. contortus from different continents were resistant to IVM, including North America [18], South America [19], Europe [20], Australasia [21], Africa [22] and Asia [23]. However, no registered cases of IVM resistance in H. contortus and IVM e cacy situation have been reported in Xinjiang.…”

Section: Discussionmentioning

confidence: 99%

First detection and evaluation of ivermectin resistance in Haemonchus contortus from sheep in Zhaosu, Xinjiang, China

Tuersong¹,

Yan²,

Zhou³

et al. 2020

Preprint

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Background: In 2017 in Zhaosu, Xinjiang, China, a series of sheep death suspected to have reduced the potency of ivermectin (IVM) have raised the vigilance of herdsmen. At the same time, veterinary practitioners warned of the potential risk of the lack of efficacy of IVM in the treatment of Haemonchus contortus. Methods: In this study, the efficacy of IVM against H. contortus was assessed under controlled experimental conditions by using larval developmental assay (LDA) and faecal egg count reduction test (FECRT) to determine whether the reduced efficacy in sheep in Zhaosu was caused by IVM resistance. Results: In total, 1560 adult worms were isolated from the abomasum of post mortem examination of dead sheep which received recent IVM (0.2 mg/kg) treatment before death and eggs were collected from female worms for usage in LDA. The LDA result revealed that significant difference in resistance ratio (RR) was detected between the IVM-susceptible and -resistant isolates (6.09 for Zhaosu strain) of H. contortus. The Zhaosu strain was maintained in laboratory for FECRT, which showed that the efficacy of IVM was 0% at 1, 2 and 3 times dose, but at 4.8 times dose, it reached 87.5% at 14 days post-treatment. Conclusion: The findings suggested that controlling H. contortus of small ruminants in Zhaosu requires an integrated parasite management strategy that should include monitoring of infection and anthelmintic resistance, and increasing the awareness of herdsmen on the importance of using the appropriate drug usage to improve nematode control in sheep.

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Seasonal Dynamics of Gastrointestinal Nematode Infections of Goats and Emergence of Ivermectin Resistance in Haemonchus contortus in Hubei Province, China

Cited by 11 publications

References 9 publications

Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus

Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus

Comparative Analysis on Transcriptomics of Ivermectin Resistant and Sensitive Strains of Haemonchus Contortus

First detection and evaluation of ivermectin resistance in Haemonchus contortus from sheep in Zhaosu, Xinjiang, China

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