Sakskoe Lake is the most thoroughly studied water body in Crimea. Its therapeutic mud and brine are exploited by balneological treatment methods. During the two centuries, when the lake was used by humans, it was subdivided into seven water bodies by dams, with two of these bodies (Eastern and Western) being now utilized for medical purposes, and the other five being protective. The Eastern (currently exploited) basin is now used to produce therapeutic mud and brine. The bottoms of the saline basins are covered by sediments: an upper layer of black silt, which is underlain by gray, steel-gray, occasionally bluish silt, which is used as therapeutic mud. The lake is fed by surface and groundwaters. Retaining dams and flood embankments erected in nearby ravines preclude desalination of the lake with flood and rain waters. Seawater is pumped to the lake through channels to preclude its drying. A quay is now actively constructed around the lake and will likely notably affect its hydrological and ecological conditions. The aim of the present study was the comparative analysis of the chemical and physical characteristics of the Eastern and Western basins of the Sakskoe Lake at the period of intensive building activity on their coasts at the summer period of 2019. Brine samples were collected at two tested sites in each of the lakes. Water salinity was measured by a PAL-06S LTA GO (Japan) refractometer and was expressed in ‰. The pH and Eh of the waters and oxygen dissolved in them were determined in the laboratory by an Expert-001 (Econix-Expert Moexa CoLtd, Russia) analyzer, with the use of appropriate Volta (Russia) selective electrodes. Ions concentration was determined spectrophotometrically and by atomic absorption method. Various living stages of Artemia populations in two water bodies were studied according the standard microscopic methods. The obtained results demonstrated, that the total content of the cations in the brine of the Western basin was in 1,5-fold higher as compared with the data of the brine in the Eastern basin (108,4 and 71,2 g/l respectively). The values were directly correlated with the high salinity of the Western basin brine. The identical trend was shown for the anions concentration, which was significantly greatly in the Western basin related to Eastern one (193,14 and 125,41 g/l correspondingly), which was connected with the different hydrogeological conditions of the formation of the ecosystems of the tested water bodies. At the other hand, pH, salinity and Eh of the brine in the Eastern basin were lower than in the brine of the Western basin, while the concentration of dissolved oxygen was higher. In the brine of the Eastern basin of the lake all living stages of Artemia, including cysts, nauplia and adults were found, while in the Western basin only cysts were shown. Therefore, according the obtained results we could suggest, that anthropogenic processes which were taken place on the coasts of the Sakskoe Lake accompanied with the intensive building activity did not influence on the lake ecosystem. The further monitoring studies including the testing of the ions concentrations in the brine of two basins, Artemia populations state are required for the understanding the changes of the both ecosystems and the possible reasons of their changes. It is important for development of the optimization of the management of Sakskoe lake and its resources exploitation.