Seasonal effect on aflatoxin M1 contamination in raw and UHT milk from Croatia

Bilandžić, Nina; Božić, Đurđica; Đokić, Mаја; Sedak, Marija; Kolanović, Božica Solomun; Varenina, Ivana; Tanković, Sanin; Cvetnić, Željko

doi:10.1016/j.foodcont.2013.12.002

Cited by 57 publications

(29 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the research conducted in Croatia during 2013, the determined concentration ranged from LOD up to 1135 ng/kg and 183.5 ng/kg, for raw milk and UHT milk, respectively (Biland zi c et al, 2014). Accordingly, the determined AFM 1 contamination in milk samples revealed over 90% non-compliancy regarding the EU regulation (EC, 2006b).…”

Section: Occurence Of Aflatoxin M 1 In Raw Milkmentioning

confidence: 98%

Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013

Dimitrieska-Stojković

Stojanovska-Dimzoska

Ilievska

et al. 2016

Food Control

View full text Add to dashboard Cite

Section: Occurence Of Aflatoxin M 1 In Raw Milkmentioning

confidence: 98%

Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013

Dimitrieska-Stojković

Stojanovska-Dimzoska

Ilievska

et al. 2016

Food Control

View full text Add to dashboard Cite

“…The AFs prevalence determined in both countries within the entire period are comparable, while results from 2012 results showed the noticeable higher prevalence of both positive samples and samples that were above EU MRL in Serbia, showing 49.9% ( n = 724) and 33%, respectively. Prevalence of positive milk samples collected in Croatia from February to July 2013 was 46.1% ( n = 3716) and 39.5% ( n = 706), with 27.8% and 9.64% of samples exceeding EU MRL for raw and UHT milk respectively [57]. Study for the period February 2013–January 2014 from Macedonia had shown the prevalence of AFM1 in raw milk of 42.4% ( n = 3636) with only 2.9% exceeding EU MRL [58].…”

Section: Principal Mycotoxins In Food and Feed In Serbiamentioning

confidence: 99%

Overview on the Mycotoxins Incidence in Serbia in the Period 2004–2016

Udovički

Audenaert

Saeger

et al. 2018

Toxins

View full text Add to dashboard Cite

With an average annual production of 6.9 M tonnes and 2.5 M tonnes of maize and wheat respectively, Serbia is one of the main grain producers and exporters in Europe. Cereals are also the major staple food in Serbian diet. In view of the high cereal consumption, for human and animal nutrition, the presence of mycotoxins entails a high public health risk of chronic exposure to mycotoxins. This study provides an overview of the incidence of predominant mycotoxins, mainly in cereal and dairy products, in Serbia, in the 2004–2016, using data reported in the scientific literature. The study demonstrated that the total prevalence of aflatoxins was 62.9% (n = 12,517) with 26.2% of the samples exceeding the EU limits during this period. Results obtained for T-2/HT-2 (n = 523), deoxynivalenol (n = 2907), fumonisins (n = 998), zearalenone (n = 689) and ochratoxin A (n = 740) indicated the prevalence of 45.5%, 42.9%, 63.3%, 39.3% and 28.1%, respectively. For these mycotoxins, the EU limits were less frequently exceeded. Comprehensive collection and analysis of all accessible information reviewed in this paper showed moderate incidence and prevalence of mycotoxins in Serbia, with an exception of the 2012 drought year and the 2014 flood year.

show abstract

“…It is also possible for milk products, like yoghurt, butter and cheese, to be contaminated with AFM 1 because of milk contamination [8,11,21]. In recent years, many researchers have reported AFM 1 contamination in raw, pasteurized and Ultra High Temperature (UHT) milk from Turkey and other countries [5,7,30,31,45] (Table 1). The potential existence of AFM 1 levels in milk and milk products is the reason they should be monitored continuously for the sake of public health [22].…”

Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxin M1 and Ochratoxin A in Raw, Pasteurized and UHT Milk in Turkey

Turkoglu

Keyvan

2019

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background:Mycotoxins produced by yeast and fungi have toxic effects on human and animal health. Aflatoxin B 1 (AFB 1 ) is the most toxic hepatocarcinogen to mammals. Aflatoxin M 1 (AFM 1 ), which has been found in milk and dairy products, is the hydroxylated metabolite of AFB 1 . Aflatoxin M 1 is formed by the cytochrome P450 enzyme in the liver. Ochratoxin A (OTA) is synthesized by Aspergillus and Penicillium species. Ochratoxin A is known to cause teratogenic, immunotoxic, nephrotoxic and carcinogenic effects. Due to the potential harmful effects on human and animal health, OTA has also been receiving increased attention globally; however, there is limited information on the presence of OTA in milk and dairy products. The aim of this study was to determine how mycotoxins impact the hygienic quality of raw and heat-processed milk. Materials, Methods & Results:In this study, a total of 105 milk samples were analyzed (35 raw, 35 pasteurized and 35 UHT) to identify AFM 1 and OTA in raw, pasteurized and ultra-high temperature processing (UHT) milk. The levels of AFM 1 were detected by using the enzyme-linked immunosorbent assay (ELISA). The milk samples were centrifuged in order to remove the fat content from the milk. After centrifugation, the upper cream layer was withdrawn with a pipette. The non-fat liquid portion was placed in wells at 100 μL for analysis. The concentration of AFM 1 in the milk samples was analyzed by AFM 1 test kit. The milk samples with AFM 1 levels greater than 50 ng/L were confirmed by using High-Performance Liquid Chromatography (HPLC). An Ochratoxin A Serum / Milk ELISA test kit was used for the analyses of OTA. The analyses were made according to the manufacturer's instructions, and samples were analyzed in duplicate. The absorbance value of milk samples was obtained from the ELISA plate reader at 450 nm. The mean value of AFM 1 was found to be 19.54 ng/L in the milk samples. According to the European Commission (EC), the maximum limit for AFM 1 in milk is 50 ng/L. In our study, eight (7.61%) of the 105 samples exceeded this limit. The mean value of OTA was found to be 119 ng/L in the milk samples. The relationship between milk type and levels of AFM 1 was found to be significant at (P < 0.01). The mean value of AFM 1 in pasteurized milk was found statistically significant and lower than raw milk (P < 0.05). The difference between levels of OTA and milk type was not statistically significant at (P > 0.05). Discussion: Milk is a great protein source especially for children in the age of growth. Yeasts such as Fusarium, Aspergillus and Penicillium produce mycotoxins that cause food, feed contamination. Owing to carcinogenic, mutagenic and teratogenic effects of AFM 1 , presence of AFM 1 in milk samples may adversely affect human health. The presence of AFM 1 in different contamination levels can be observed in milk and milk products. Factors such as ration type, climate conditions, feed storage conditions, feeding regime and health status of dairy animals may be effective in the occurrenc...

show abstract

Seasonal effect on aflatoxin M1 contamination in raw and UHT milk from Croatia

Cited by 57 publications

References 37 publications

Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013

Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013

Overview on the Mycotoxins Incidence in Serbia in the Period 2004–2016

Determination of Aflatoxin M1 and Ochratoxin A in Raw, Pasteurized and UHT Milk in Turkey

Contact Info

Product

Resources

About