Seasonal expression of insulin-like growth factor 1 (IGF-1), its receptor IGF-1R and klotho in testis and epididymis of the European bison (Bison bonasus, Linnaeus 1758)

Tabęcka-Łonczyńska, Anna; Mytych, Jennifer; Sołek, Przemysław; Kowalewski, Mariusz P; Koziorowski, Marek

doi:10.1016/j.theriogenology.2018.12.016

Cited by 5 publications

(4 citation statements)

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“…Wisent reproduction has been discussed in several articles [17][18][19][20][21][22][23][24][25]. However, there is a paucity of information focused on detailed characteristic of the male gamete in context of cryopreservation process in this species [26].…”

Section: Introductionmentioning

confidence: 99%

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

et al. 2022

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Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaired or dead spermatozoa have lower tolerance for cryopreservation process. Such samples are prone to involve damage acrosomes, to produce and release reactive oxygen which negatively affects proper function of spermatozoas. It is a good practice to select intact and viable gametes before subjecting the sample to cryopreservation to improve the efficiency of this process. The aim of this study was to assess the ability of Percoll® density gradient centrifugation in order to improve the quality of wisent spermatozoa after cryopreservation. Spermatozoa samples were analysed with computer-assisted semen analysis system and flow cytometry. Results Percoll® density gradient centrifugation resulted in increased percentage of motile spermatozoa, higher proportion of spermatozoa with normal morphology and proper functionality but also in a significant reduction of the total number of gametes. Nevertheless, the concentration of frozen spermatoza was still sufficient for obtaining a few complete insemination doses suggested for cattle from each epididymis. Conclusions While creating a high-quality genetic reserve, for in vitro fertilisation purposes, eliminating detritus and improving the overall quality of samples is more important than total number of spermatozoa. For these reasons, the achievement of higher post thaw quality of spermatozoa justifies the purification of samples by centrifugation in a Percoll® density gradient prior to the cryopreservation process.

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Section: Introductionmentioning

confidence: 99%

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

et al. 2022

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“…Insulin-like growth factor-1 is a low-molecular-weight peptide, produced by liver and by many other types of cells, and it plays an important role in regulating postnatal growth, mammary gland development, lactation and reproduction in male and female in several species ( Jiang & Lucy, 2001 ; Lammers, Heinrichs & Kensinger, 1999 ; Renaville, Hammadi & Portetelle, 2002 ; Tabecka-Lonczynska, Mytych, Solek, Kowalewski & Koziorowski, 2019 ). For these important physiological actions, this molecule has received and still does receive considerable attention from researchers, as it can affect the animal's productive career.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep

Luridiana

Mura

Veronica

et al. 2020

Veterinary and Animal Science

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Highlights IGF-1 gene plays an important role in regulating reproduction in ruminants. Here three SNPs were found in the IGF-I gene in Sarda sheep. The found SNPs were related to reproductive performance and milk yield. This results could be used to improve the reproductive performances and milk yield in Sarda sheep.

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“…The IGFβ receptor (IGF-IR β), is necessary to maintain the activity of insulin-like growth factor [ 41 ] and in our study, the level of protein expression was decreased in almost all tested groups. Only in the NS-398/LPS and MTEP + NS-398/LPS groups was a decrease not observed.…”

Section: Discussionmentioning

confidence: 99%

Trade‐offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model

et al. 2021

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The increasing number of depression cases leads to a greater need for new antidepressant treatment development. It is postulated that antidepressants may harm male fertility, but the cellular mechanism is still poorly understood. The role of growth factors and klotho protein in maintaining normal male reproductive function is well documented. Hence, the study aimed to investigate the effect of the antidepressant drug – imipramine (tricyclic AD), and other substances with antidepressant potential (ALS), administered in combination or in combination with LPS (an animal model of depression) on gene expression and protein synthesis of IGF-2 (insulin-like growth factor 2), TGF-β1 (transforming growth factor β1), NGF (nerve growth factor), KGF (keratinocyte growth factor) and protein synthesis of VEGF-A (vascular endothelial growth factor A), IGF-IR (insulin-like growth factor receptor 1), EGFR (epidermal growth factor receptor) and klotho in the testis of mice. Mice were injected intraperitoneally with selected ALS and LPS or 10% DMSO (controls) (n = 7/group) once a day for 14 days. Animals were decapitated and testes collected for RNA and protein purification. PCR and western blot methods were employed for the evaluation of growth factors and klotho expression. The results obtained indicated a decreased level of most of the analyzed genes and proteins, except KGF; its expression increased after treatment with MTEP and IMI administrated individually and after NS-398, and IMI in combination with LPS. Our results may suggest that the tested ALS and LPS can contribute to a reduction of male fertility, but NS-398, IMI, and IMI+NS-398 may also act as stimulants after LPS.

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Seasonal expression of insulin-like growth factor 1 (IGF-1), its receptor IGF-1R and klotho in testis and epididymis of the European bison (Bison bonasus, Linnaeus 1758)

Cited by 5 publications

References 53 publications

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep

Trade‐offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model

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