In the present work, the variations in Total Electron Content (TEC) induced by changes in the ionizing radiation of the Sun, which are related to the rotation period (about 27 days), were investigated. This study was based on a 30-year period. The relative deviations in the TEC and F10.7 values were used in the data analysis. The use of this modification aimed to eliminate the stationary diurnal, seasonal, and solar course of the TEC over the course of the long-term variations in solar activity, preserving the variations within a time scale of 27 days and less. As a result, the values of the linear regression coefficient between the relative deviations in the two considered quantities from the median (quiet conditions) for one rotation period were obtained. Depending on the general level of solar activity, the season, and the latitude, this coefficient varied between 40% and 60%. The analysis showed that the minimum values were observed during high solar activity. The latitudinal distribution demonstrated an increase in the area of the Equatorial Ionization Anomaly (EIA) under the influence of the so-called “fountain effect”. As a result, there was a seasonal variation and an increase in the winter months at mid and high latitudes and a decrease in the months of the minimum zenith angle of the Sun at low latitudes. A well-pronounced asymmetry in the equinox months was also obtained. The obtained results are the novelty of this study and can be used to improve empirical models for short-term TEC forecasting.