Seasonal genetic variation associated with population dynamics of a poecilogonous polychaete worm

Thonig, Anne; Banta, Gary Thomas; Hansen, Benni Winding; Knott, K. Emily

doi:10.1002/ece3.3518

Cited by 8 publications

(9 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA was extracted from whole host individuals using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer's protocol for animal tissue and stored in −20°C (Thonig et al, 2017), and DNA concentration was measured with Qubit 4.0 Fluorometer with 1X dsDNA HS Assay (Thermo Fisher Scientific). In the current study, a subset of those samples was analyzed with ddPCR (Table 1).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts

Hiillos

Thonig

Knott

2021

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

Interactions between symbiotic organisms and their hosts dramatically influence not only organismal ecology and evolution, but also the dynamics of entire ecosystems (Faust & Raes, 2012;Godfrey-Smith, 2015).For the majority of symbioses, however, we do not know how the interacting species affect each other, much less the broader consequences of the symbiosis for communities or ecosystems. Often, it is the lack of tools for effectively monitoring or investigating symbiotic interactions that prohibits progress. In addition, a lack of general knowledge

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Survey Of Host Populationsmentioning

confidence: 99%

Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts

Hiillos

Thonig

Knott

2021

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we examined marine apicomplexan infections, coinfections, and their associations with host genetic diversity in three natural populations of the polychaete worm, Pygospio elegans . We determined genetic diversity by genotyping individual hosts at seven neutrally evolving microsatellite loci (Thonig et al, 2017 ) and inspected infection patterns of an undescribed marosporidian, Rhytidocystis sp., and the gregarine species, Selenidium pygospionis , that live in symbiosis with the host. Our aim was to determine whether infection dynamics are associated with differences in population genetic diversity and heterozygosity among individual hosts.…”

mentioning

confidence: 99%

Coinfection patterns of two marine apicomplexans are not associated with genetic diversity of their polychaete host

Hiillos

Rony

Rueckert

et al. 2022

J Eukaryotic Microbiology

Self Cite

View full text Add to dashboard Cite

Coinfections of two or more parasites within one host are more of a rule than an exception in nature. Interactions between coinfecting parasites can greatly affect their abundance and prevalence. Characteristics of the host, such as genetic diversity, can also affect the infection dynamics of coinfecting parasites.Here, we investigate for the first time the association of coinfection patterns of two marine apicomplexans, Rhytidocystis sp. and Selenidium pygospionis, with the genetic diversity of their host, the polychaete Pygospio elegans, from natural populations. Host genetic diversity was determined with seven microsatellite loci and summarized as allelic richness, inbreeding coefficient, and individual heterozygosity. We detected nonsignificant correlations between infection loads and both individual host heterozygosity and population genetic diversity.Prevalence and infection load of Rhytidocystis sp. were higher than those of S. pygospionis, and both varied spatially. Coinfections were common, and almost all hosts infected by S. pygospionis were also infected by Rhytidocystis sp. Rhytidocystis sp. infection load was significantly higher in dual infections.Our results suggest that factors other than host genetic diversity might be more important in marine apicomplexan infection patterns and experimental approaches would be needed to further determine how interactions between the apicomplexans and their host influence infection.

show abstract

“…It is also important to consider the context, and the life history of the focal species when assessing SGDCs. Since genetic diversity in species with short life spans can have seasonal fluctuations [ 68 ], spatial SGDCs based on such species could lead to non-significant results, no SGDC or even skewed conclusions, raising the question if the theory is appropriate for these species. SGDCs assessed using species with annual lifecycles on a temporal scale will, however, provide information about responses to seasonal environmental changes and population dynamics.…”

Section: Discussionmentioning

confidence: 99%

Species and genetic diversity relationships in benthic macroinvertebrate communities along a salinity gradient

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Species- and genetic diversity can change in parallel, resulting in a species-genetic diversity correlation (SGDC) and raising the question if the same drivers influence both biological levels of diversity. The SGDC can be either positive or negative, depending on whether the species diversity and the genetic diversity of the measured species respond in the same or opposite way to drivers. Using a traditional species diversity approach together with ultra-conserved elements and high throughput sequencing, we evaluated the SGDCs in benthic macrofauna communities in the Baltic Sea, a geologically young brackish water sea characterised by its steep salinity gradient and low species richness. Assessing SGDCs from six focal marine invertebrate species from different taxonomic groups and with differing life histories and ecological functions on both a spatial and temporal scale gives a more comprehensive insight into the community dynamics of this young ecosystem and the extrinsic factors that might drive the SGDCs. Results No significant correlations between species diversity and genetic diversity were found for any of the focal species. However, both negative and positive trends of SGDCs for the individual focal species were observed. When examining the environmental drivers, no common trends between the species were found, even when restricting the analysis to specific taxonomic classes. Additionally, there were no common environmental factors driving the diversity relationships for species sharing the same SGDC trend (positive or negative). Local population dynamics, together with the invasion history of the individual species and their unique adaptation to the distinctive environment of the Baltic Sea, are expected to be of major influence on the outcome of the SGDCs. Conclusions The present results highlight the importance of assessing SGDCs using multiple species, not just a single indicator species. This emphasises a need to pay attention to the ecology and life history of the focal species. This study also provides insight into the large differences in both patterns and drivers of genetic diversity, which is important when including genetic biodiversity in conservation plans. We conclude that the effects of environmental and biological factors and processes that affects diversity patterns at both the community and genetic levels are likely species dependent, even in an environment such as the Baltic Sea with strong environmental gradients.

show abstract

Seasonal genetic variation associated with population dynamics of a poecilogonous polychaete worm

Cited by 8 publications

References 59 publications

Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts

Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts

Coinfection patterns of two marine apicomplexans are not associated with genetic diversity of their polychaete host

Species and genetic diversity relationships in benthic macroinvertebrate communities along a salinity gradient

Contact Info

Product

Resources

About