Studies were conducted to investigate the effect of different levels of nitrogen fertilizer on the incidence of whitefly in six Bt cotton cultivars, namely, Ankur 3028, NCS 855, RCH 776, RCH 650, RCH 773, Bioseed 6588 and one each of American cotton LH2108 and desi cotton cultivar FDK 124 at the Entomological Research Farm, Department of Entomology, PAU, Ludhiana during 2014 and 2015. The study revealed that incidences of whitefly were higher during 2015 than 2014 during the course of study. During both years, significantly higher incidence of whitefly was recorded at higher dose of nitrogen fertilizer (325 kg/ acre) as compared to lower doses (165 and 250 kg/ acre). Among different cultivars, whitefly was significantly higher on Bt cotton cultivars as compared to non-Bt and desi cotton cultivar. Whitefly population was significantly higher on Bt cotton cultivar, Bioseed 6588 (6.10 and 8.84/ 3 leaves) during 2014 and 2015, respectively. However, lower population of whitefly was recorded on the desi cotton cultivar, FDK 124 (3.54 and 2.95/ 3 leaves) during 2014 and 2015, respectively. The correlation coefficient studies with weather parameter revealed that adults showed significant positive correlation with maximum and minimum temperature, evaporation and sunshine hours.