Abstract. Mammoth teeth have been widely investigated using stable-isotopic analysis
for paleoenvironmental and paleoecological reconstructions due to their
large size and frequent discoveries. Many past investigations sampled the
tooth enamel with the “bulk” method, which involves drilling one sample from
the occlusal surface to the root for each tooth. Some of the more recent
studies applied the “sequential” method, with a sequence of samples drilled
following the dominant enamel growth direction to produce a time series of
isotopic oscillations that reflects high-resolution environmental changes, as
well as changes in mammoth dietary behavior. Although both the bulk and
mean sequential δ18O values are expected to represent the
averaged signal over the time of tooth formation, it is uncertain whether
their paleoenvironmental records were formed during similar periods of time.
In this study, we applied both sampling methods (sequential drilling first
followed by a thin layer of bulk drilling) on the same enamel ridges of
multiple mammoth teeth and compared their respective δ18O
values. The results indicated that, in most enamel ridges, the bulk samples
have more negative δ18O values compared to the average
sequential values, and some of the bulk values even fall outside the range
of sequential values. The most likely explanation for the differences is the
structure and formation stages of enamel that caused uneven distributions of
different seasons recorded in the samples. This finding provides insights
into current limitations of the two sampling methods and the applicability
of cross-method data comparison from past studies.