Seasonal studies of methanogenesis and desulfurication in sediments of the river saar

Zaiss, Ulrich

doi:10.1016/s0721-9571(81)80020-8

Cited by 16 publications

(14 citation statements)

References 25 publications

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“…Reviewed data indicate that the contribution of acetate to methanogenesis in deep lake sediments varies between 25 and 75% [24]. However, other studies showed that the contribution may be as low as 4% in river sediments [25] and as high as 96% in an acidic lake sediment [26]. The reasons for this large variability are still unknown.…”

Section: Discussionmentioning

confidence: 99%

Methane production in littoral sediment of Lake Constance

Thebrath

Rothfuß

Whiticar

et al. 1993

FEMS Microbiology Letters

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Maximum rates of CH4 production in the littoral sediment were observed in 2–5 cm depth. The CH4 production rates increased during the year from about 5 mmol m−2d−1 in December to a maximum of about 95 mmol m−2d−1 in September. CH4 production rates showed a temperature optimum at 30°C and an apparent activation energy of 76 kJ mol−1. A large part of the seasonality of CH4 production could be ascribed to the change of the sediment temperature. Most of the produced CH4 was lost by ebullition. Gas bubbles contained about 60–70% CH4 with an average δ13C of −56.2% and δD of −354%, and 2% CO2 with an average δ13C of −14.1% indicating that CH4 was produced from methyl carbon, i.e. mainly using acetate as methanogenic substrate. This result was confirmed by inhibition of methanogenesis with chloroform which resulted in an accumulation rate of acetate equivalent to 81% of the rate of CH4 production. Most probable numbers of methanogenic bacteria were in the order of 104 bacteria g−1d.w. sediment for acetate‐, methanol‐ or formate‐utilizing, and of 105 for H2‐utilizing methanogens. The turnover times of acetate were in the order of 2.3–4.8 h which, with in situ acetate concentrations of about 25–50 μM, resulted in rates of acetate turnover which were comparable to the rates of CH4 production. The respiratory index (RI) showed that [2−14C]acetate was mainly used by methanogenesis rather than by respiratory processes, although the zone of CH4 production in the sediment overlapped with the zone of sulfate reduction.

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Section: Discussionmentioning

confidence: 99%

Methane production in littoral sediment of Lake Constance

Thebrath

Rothfuß

Whiticar

et al. 1993

FEMS Microbiology Letters

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“…Ignition loss was measured after heating at 550 ‡C for 2 h. Methane in sediments was determined by headspace analysis [12] modi¢ed by purging methane into a headspace in 15-ml vials containing 1 ml of sediment and 4 ml of saturated salt water. Ignition loss was measured after heating at 550 ‡C for 2 h. Methane in sediments was determined by headspace analysis [12] modi¢ed by purging methane into a headspace in 15-ml vials containing 1 ml of sediment and 4 ml of saturated salt water.…”

Section: Measurements Of Some Characteristics Of Sediment Samplesmentioning

confidence: 99%

Vertical distributions of sulfate-reducing bacteria and methane-producing archaea quantified by oligonucleotide probe hybridization in the profundal sediment of a mesotrophic lake

Koizumi

Takii

Nishino

et al. 2003

FEMS Microbiology Ecology

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Vertical distributions of sulfate-reducing bacteria and methane-producing archaea were investigated in the profundal sediment of a freshwater lake using membrane-immobilized small subunit rRNA hybridization with group- and genus-specific oligonucleotide probes. The annual average of the relative abundance of small subunit rRNA hybridized with all probes for sulfate-reducing bacteria to total small subunit rRNA was 2.3% at 0-2 cm and increased with depth up to 22.9% at 8-14 cm where sulfate concentration was less than 10 nmol ml(-1) in interstitial water, suggesting that these bacteria may survive on alternative metabolisms. The signal of probe Dsv687 (the family Desulfovibrionaceae and some Geobacteraceae) was the main factor in this increase. The relative abundance of methane-producing archaea to total small subunit rRNA was highest (7.8%) at 8-14 cm, dominated by the order Methanosarcinales. The metabolic rates measured in the sediments demonstrated that the peaks of sulfate reduction and methane production were separated vertically, and were not linked to their small subunit rRNA distributions. Our data indicate that sulfate-reducing bacteria can coexist with methane-producing archaea from 0 to 20 cm in the freshwater lake sediment.

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“…Approx. 1.6 × 105 methanogens per g wet sediment were found by applying a most-probablenumber method [13], whereas 5 × 104 methanogens per ml wet sediment were found by determining the number of colony-forming units [14] and 105-109 methanogens per g dry sediment were estimated by means of a direct fluorescent-antibody technique [15].…”

Section: Contribution Of Amoeba-derived Methanogens To Total Methane mentioning

confidence: 99%