Seasonal timing of sperm production in roe deer: interrelationship among changes in ejaculate parameters, morphology and function of testis and accessory glands

Goeritz, Frank; Quest, M; Wagener, Asja; Fassbender, M; Broich, Alexandra; Hildebrandt, Thomas B.; Hofmann, R. R.; Blöttner, S.

doi:10.1016/s0093-691x(02)01201-3

Cited by 113 publications

(82 citation statements)

References 43 publications

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“…The frequency of several abnormalities differed between the nonbreeding and breeding seasons in roe deer [6]. In this study and the study of captive bear reported by Kojima et al [16], collection of semen was performed in estimated mating season [36].…”

Section: Fresh Semenmentioning

confidence: 76%

Electroejaculation and Semen Cryopreservation of Free-Ranging Japanese Black Bears (Ursus thibetanus japonicus)

Okano

Murase

Tsubota

2004

The Journal of Veterinary Medical Science

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ABSTRACT. The Japanese black bear (Ursus thibetanus japonicus) is endangered for extinction in some areas of Japan, and semen collection and cryopreservation are an important means to preserve genetic resources. The aim of this study was to characterize and c ryopreserve semen of free-ranging Japanese black bears. Semen was collected by electroejaculation procedure from 4 free-ranging Japanese black bears at the capture point in the field. Ejaculates containing motile sperm were recovered from all of the animals and ejaculate volume, total sperm count, % motility (percentage of motile spermatozoa), % viability (percentage of spermatozoa that excluded eosin) and % abnormal morphology (range (mean)) were 0.65-2.20 (1.51) ml, 99-1082 (490) × 10 6 , 5-100 (31), 42-97 (66) and 20-87 (53), respectively. Three of the 4 ejaculates were diluted with an egg yolk-TRIS-citrate-glucose extender and cryopreserved in liquid nitrogen. Motile spermatozoa were observed after freezing and thawing in all cases. This study showed that electroejaculation would be a useful method for collecting semen from free-ranging Japanese black bears and that at least motile spermatozoa would be obtained by fre ezing the thus collected electroejaculates. KEY WORDS: cryopreservation, electroejaculation, free-ranging, Japanese black bear, semen.J. Vet. Med. Sci. 66(11): 1371-1376, 2004 The Japanese black bear (Ursus thibetanus japonicus) is a large mammal that inhabits the islands of Honshu and Shikoku in Japan. In recent years, their habitats have become fragmented and shrunken by human activity, and this species even faces extinction in some areas [7]. What is worse, bears have been killed to protect forestry, agriculture, livestock and humans from damage caused by them.Endangered and threatened wild animals are now attracting attention as a genetic resource and for species conservation, and semen has been recovered from various captive non-domestic animals, including fat-tailed dunnarts, koalas, brushtail possums, long-footed potoroos, northern brown bandicoots and ring-tailed possums [32] [4,20,22,24], Hokkaido brown bears [13,14] and Japanese black bears [16]. However, these studies were done with captive animals, and there are no reports on semen collection and cryopreservation in free-ranging bears. Cryopreservation of semen collected from free-ranging animals is one of the most important methods for preservation of genetic resources and useful for artificial breeding. Moreover, characteristics of semen from wild animals may be essential for understanding reproductive status under field conditions, although studies using captive animals, kept under controlled conditions, may provide valuable data to analogize state under field conditions. It is easy to expect that the state of captive animals may differ from that of free-ranging ones to some extent, because of differences in their living environment and diet. It is difficult to obtain semen from free-ranging Japanese black bears due to limited access to the animals and seasonality of their ...

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Section: Fresh Semenmentioning

confidence: 76%

Electroejaculation and Semen Cryopreservation of Free-Ranging Japanese Black Bears (Ursus thibetanus japonicus)

Okano

Murase

Tsubota

2004

The Journal of Veterinary Medical Science

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“…For gene-expression analysis, roe deer testis tissue was obtained monthly (except February) by fine-needle biopsies from five free-ranging animals immediately after capture as described by Göritz et al (2003). The biopsies were immediately put into 20 µl TRIzol reagent (GIBCO Life Technologies) and snap-frozen in liquid nitrogen.…”

Section: Animals and Tissuesmentioning

confidence: 99%

Seasonal variation in expression and localization of testicular transforming growth factors TGF-β1 and TGF-β3 corresponds with spermatogenic activity in roe deer

Wagener¹,

Fickel²,

Schön³

et al. 2005

Journal of Endocrinology

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Adult roe deer males show hormonally controlled seasonal cycles of testicular growth and involution. Mediation of endocrine signals likely requires variable production of testicular growth factors for regulation of testis function. Here we studied the expression pattern of transforming growth factors (TGFs) 1 and 3. Total RNA from testis parenchyma was extracted monthly and analysed using quantitative reverse transcriptase PCR. The localization of mRNAs was determined by in situ hybridization, and corresponding proteins were visualized immunohistochemically. Both factors showed different expression levels and different seasonal expression patterns. The TGF-1 mRNA content was up to 45 times higher than that of TGF-3. Compared with its lowest level in May, TGF-1 expression was slightly enhanced during pre-rut (June/ July). TGF-3 expression increased 5-fold from April to June/July and decreased thereafter to its low in December. This corresponded with changing numbers of spermatocytes and round spermatids, in which both TGF-3 mRNA and the protein were mainly localized. The TGF-1 mRNA was found in interstitial cells, mainly during the non-breeding season, but also in spermatocytes and spermatids during activated spermatogenesis. The translation product was localized in few spermatogenic cells only. The results suggest that TGF-1 and -3 are important in regulating seasonal spermatogenesis of roe deer with diverse functions affecting interstitial and spermatogenic cells.

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“…Procesom tym towarzyszą zmiany światła kanalików plemnikotwórczych, co uwarunkowane jest wahaniami aktywności mitotycznej i mejotycznej komórek kanalików nasieniotwórczych. Wielkość jąder oraz liczba komórek Sertoliego niejednokrotnie wykorzystywane są jako miernik procesów spermatogenezy, w aspekcie ilości i jakości plemników (3,11,14,18,19,22,24).…”

Section: Praca Oryginalnaunclassified

“…Pierwsza z nich obejmowała samce pozyskane w okresie od 11 maja do 30 maja, zaś druga od 20 lipca do 20 sierpnia. Pierwszy z wyodrębnionych przedziałów czasowych obejmował okres przedrujowy, zaś drugi szczytowy okres rui u tego gatunku (11,18,23). Dodatkowo z pozyskanego materiału wyodrębniono 4 grupy wiekowe.…”

Section: Materiał I Metodyunclassified

“…W czasie rui i okresie bezpośrednio ją poprzedzającym, w związku z intensywnym przebiegiem procesów spermatogenezy, obserwowany jest wyraźny wzrost wielkości i masy jąder, przy jednoczesnym spadku masy ciała (3,10,11,13,18,24) spowodowanym, jak się wydaje, intensywnym poszukiwaniem samic będących w rui, pilnowaniem własnego rejonu oraz walkami z innymi kozłami, potencjalnymi rywalami. Badania prowadzone w rejonie Wielkopolski w 2012 r. wykazały spadek masy ciała u kozłów pomiędzy majem a wrześniem na poziomie 1,1 kg.…”

Section: Ryc 1 Udział Plemników Prawidłowych I Z Wadami W Lewych I unclassified

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Differentiation in body and testes weight as well as sperm quality of male roe deer from the Lublin Upland in the reproductive season

Flis

Wrona

Nozdryn-Płotnicki

2017

Medycyna Weterynaryjna

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Flis M., Wrona Z., Nozdryn-Płotnicki Z.Differentiation in body and testes weight as well as sperm quality of male roe deer from the Lublin Upland in the reproductive season Summary The aim of the study was to evaluate the morphology structure of sperm cells and quality of individual male deer obtained in the Lublin Upland during the 2015/2016 hunting season. The comparison included two separate periods of the hunting season resulting from varying physiological processes related to the estrus period of this species. In the first study with regards to the estrus period, males were characterized by an average of 1.6 grams less testicle weight, while body weight was 1.9 kg higher. Increasing the mass of the testicles in the reproductive season proceeded simultaneously with the increase in the share of properly developed sperm cells to the level of 70.6%. During the pre-estrus period, the percentage of spermatozoids with main and secondary defects accounted for almost 60%. Between the compared periods, an approximately 6.8% body weight loss occurred. The highest percentage of properly developed spermatozoids was present in deer aged 4 and 5 years. Regarding the greatest body weight loss in these two age groups, it is indicated that this age is the climax in terms of potential accession to the breeding and effective mating of females and the possibility of fertilization. Analysis of the relationship between body weight and testicle weight showed low and statistically insignificant correlation coefficients (r xy = 0.331-0.393) between those features.

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Seasonal timing of sperm production in roe deer: interrelationship among changes in ejaculate parameters, morphology and function of testis and accessory glands

Cited by 113 publications

References 43 publications

Electroejaculation and Semen Cryopreservation of Free-Ranging Japanese Black Bears (Ursus thibetanus japonicus)

Electroejaculation and Semen Cryopreservation of Free-Ranging Japanese Black Bears (Ursus thibetanus japonicus)

Seasonal variation in expression and localization of testicular transforming growth factors TGF-β1 and TGF-β3 corresponds with spermatogenic activity in roe deer

Differentiation in body and testes weight as well as sperm quality of male roe deer from the Lublin Upland in the reproductive season

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