Tropical Cyclone (TC) Seroja is a unique tropical cyclone that has significant impacts along its path, such as floods in East Nusa Tenggara and high waves along the southern coast of Indonesia. Research related to ocean responses to tropical cyclones in Indonesia is still limited due to its rarely occurence in Indonesian waters. The responses of the upper ocean to TC Seroja were investigated using multi-satellite remote sensing of sea surface wind (SSW), sea surface temperature (SST), sea surface height anomaly (SSHA), and numerical model of mixed layer depth (MLD) and chlorophyll-a (Chl-a). The SST cooling occurred around the TC Seroja track at 0.5 – 3°C after the storm had passed. During April 3 – 7, 2021, in addition to spatial SST cooling, changes in chlorophyll-a, SSHA, and MLD were also detected. The chlorophyll-a increase to 2.57 mg/m3 and SSHA reached -10 cm. Thus, the MLD was deeper around the eye of the storm during the cyclone and became uniform after the storm passed. These characteristics indicate the upwelling phenomenon induced by the cyclone.