Seasonal variation in antibiotic resistance genes and bacterial phenotypes in swine wastewater during three-chamber anaerobic pond treatment

Wang, Yi; Sutton, Nora B.; Zheng, Yaming; Hua, Dong; Rijnaarts, H.H.M.

doi:10.1016/j.envres.2022.114495

Cited by 11 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Thauera had a greater relative abundance in the warmest samplings in Lagoon, while Jeotgalibaca decreased in Raw during the winter ( Figure 3 ). Other studies have reported this seasonality in the microbial community of different DE and wastewater treatment systems and attributed these changes mostly to environmental temperatures [ 38 , 45 , 47 ].…”

Section: Discussionmentioning

confidence: 91%

Differences in Bacterial Communities and Pathogen Indicators of Raw and Lagoon-Stabilized Farm Dairy Effluents

Illarze,

del Pino,

Irisarri

2024

Microorganisms

View full text Add to dashboard Cite

One practice for handling farm dairy effluent (DE) comprises recycling them to the soil with the challenge of balancing the tradeoff associated with environmental pollution through nutrient and microorganism loading. This study investigated seasonal bacterial community composition, diversity, abundance, and pathogenic indicators in untreated (Raw) and lagoon-stabilized (Lagoon) DE. The correlation between bacterial profiles and DE physicochemical characteristics was also analyzed. Pathogen-indicator bacteria were studied by enumerating viable counts and the bacterial community structure by 16S rRNA gene sequence analysis. Lagoon storage effectively reduced total solids (64%), suspended solids (77%), organic carbon (40%), and total nitrogen (82%), along with total coliforms, Escherichia coli, and enterococci. However, this efficiency was compromised in winter. Lagoon and Raw sample bacterial communities presented different compositions, with several environmental variables correlating to microbial community differences. Lagoon-treated DE exhibited the most diverse bacterial community, dominated by Firmicutes (40%), Proteobacteria (30%), and Bacteroidota (7.6%), whereas raw DE was mainly composed of Firmicutes (76%). Regardless of the season, dominant genera included Trichococcus, Romboutsia, Corynebacterium, and Paeniclostridium. Overall, the study emphasizes the importance of lagoon treatment for DE stabilization, showcasing its role in altering bacterial community composition and mitigating environmental risks associated with pathogens and nutrients, particularly in summer.

show abstract

Section: Discussionmentioning

confidence: 91%

Differences in Bacterial Communities and Pathogen Indicators of Raw and Lagoon-Stabilized Farm Dairy Effluents

Illarze,

del Pino,

Irisarri

2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Thus, the results indicated that potential changes in microbial communities due to microaeration and iron dosing could be the critical factor influencing the proliferation of ARGs/MGEs. However, caution should be exercised when interpreting such correlations, as the composition of bacterial communities often follows seasonal patterns . Although the composition of ARGs does not typically show such seasonality, the absolute abundance of ARGs in incoming sludge may vary between winter and summer months .…”

Section: Resultsmentioning

confidence: 99%

“…However, caution should be exercised when interpreting such correlations, as the composition of bacterial communities often follows seasonal patterns. 58 Although the composition of ARGs does not typically show such seasonality, the absolute abundance of ARGs in incoming sludge may vary between winter and summer months. 59 Thus, the temporal patterns of microbial taxonomy and function may be asynchronous or uncoupled and warrant further investigation.…”

Section: Acs Esandt Watermentioning

confidence: 99%

Bench-Scale Liquid-Phase Microaeration and Iron Dosing Comparison for In Situ Biogas Desulfurization and Antibiotic Resistance Management in Mesophilic Anaerobic Digestion

Mirsoleimani Azizi,

Zakaria,

Haffiez

et al. 2023

ACS EST Water

View full text Add to dashboard Cite

Microaeration and iron dosing are promising in situ desulfurization methods for anaerobic digestion (AD). However, limited reports exist on their systematic comparison. Consequently, this study investigated liquid-phase microaeration (60–106 mL air/Lreactor/d) and ferric chloride (250–650 mg FeCl3/L) dosages in mesophilic AD of sewage sludge operated at a solid residence time of 20 days. Both methods provided up to 88% H2S removal and moderate siloxane removal (30–37%) but had no apparent impact on mercaptan removal from biogas. However, the control and test reactors demonstrated equivalent AD performance in terms of biomethane yields, chemical oxygen demand, and total/volatile solids degradation efficiencies, indicating that air and iron dosages were primarily utilized for biogas desulfurization. Still, both methods resulted in substantial changes in microbial community composition. Notably, the indication of syntrophy between facultative microbes and hydrogenotrophic methanogens was intensified by both methods. For both methods, antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances in digestate decreased considerably due to the decrease in the abundance of potential ARG hosts. Compared to iron dosing, MGE removal was slightly higher in the microaeration reactor. These findings provide valuable insights into the efficacy of these two approaches in desulfurization and the reduction of antibiotic resistance in AD.

show abstract

“…However, pigs are considered one of the biggest reservoirs of AMR [65,[75][76][77], mainly due to the inappropriate use of antimicrobials. In some countries, the administration of antimicrobials to promote animal growth is still allowed [78][79][80]. Excessive use of antimicrobials applies a selective pressure that leads to the development of antimicrobial-resistant bacterial populations, which may later be transmitted to humans [79].…”

Section: The Role Of Meat In the Transmission Of Antimicrobial Resist...mentioning

confidence: 99%

Antimicrobial Resistance in Bacteria from Meat and Meat Products: A One Health Perspective

Conceição,

Queiroga,

Laranjo

2023

Microorganisms

View full text Add to dashboard Cite

According to the 2030 Agenda of the United Nations, one of the sustainable development goals is to ensure sustainable consumption and production patterns. The need to ensure food safety includes, other than microbiological hazards, concerns with antimicrobial-resistant (AMR) bacteria. The emergence of resistant bacteria in the food industry is essentially due to the abusive, and sometimes incorrect, administration of antimicrobials. Although not allowed in Europe, antimicrobials are often administered to promote animal growth. Each time antimicrobials are used, a selective pressure is applied to AMR bacteria. Moreover, AMR genes can be transmitted to humans through the consumption of meat-harbouring-resistant bacteria, which highlights the One Health dimension of antimicrobial resistance. Furthermore, the appropriate use of antimicrobials to ensure efficacy and the best possible outcome for the treatment of infections is regulated through the recommendations of antimicrobial stewardship. The present manuscript aims to give the current state of the art about the transmission of AMR bacteria, particularly methicillin-resistant S. aureus, ESBL-producing Enterobacteriaceae, and vancomycin-resistant Enterococcus spp., along with other ESKAPE bacteria, from animals to humans through the consumption of meat and meat products, with emphasis on pork meat and pork meat products, which are considered the most consumed worldwide.

show abstract

Seasonal variation in antibiotic resistance genes and bacterial phenotypes in swine wastewater during three-chamber anaerobic pond treatment

Cited by 11 publications

References 51 publications

Differences in Bacterial Communities and Pathogen Indicators of Raw and Lagoon-Stabilized Farm Dairy Effluents

Differences in Bacterial Communities and Pathogen Indicators of Raw and Lagoon-Stabilized Farm Dairy Effluents

Bench-Scale Liquid-Phase Microaeration and Iron Dosing Comparison for In Situ Biogas Desulfurization and Antibiotic Resistance Management in Mesophilic Anaerobic Digestion

Antimicrobial Resistance in Bacteria from Meat and Meat Products: A One Health Perspective

Contact Info

Product

Resources

About