Seasonal variation in the effects of Mediterranean plant extracts on the exsheathment kinetics of goat gastrointestinal nematode larvae

Azaizeh, Hassan; Mreny, Raghda; Markovics, Alex; Muklada, H.; Glazer, I.; Landau, S.

doi:10.1016/j.smallrumres.2015.08.004

Cited by 12 publications

(12 citation statements)

References 34 publications

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“…Lentisk ( P. lentiscus ) exhibited the highest activity on LEIA (IC 50 = 27.8–29.7 µg mL −1 ) and egg hatching processes (IC 50 = 197.7 and 223.9 µg mL −1 ), without significant differences between GIN species. Lentisk is an evergreen shrub with high polyphenol content and previous results have shown both in vitro and in vivo anthelmintic properties 29 – 32 . In previous studies, P. lentiscus extracts (acetone, ethanol and/or water) exhibited less than 20% larvae exsheathment and migration at 1200 µg mL −1 29 , 31 .…”

Section: Resultsmentioning

confidence: 99%

Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

Oliveira

Lima

Ketavong

et al. 2021

Sci Rep

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Strategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scaffolds on plants, yet salt-tolerant plants remain overlooked. This study aims to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt-tolerant plant species and tested against Haemonchus contortus and Trichostrongylus colubriformis by the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus and Helychrisum italicum picardi were the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with polyvinylpolypyrrolidone (PVPP), and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic effects were identified and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.

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Section: Resultsmentioning

confidence: 99%

Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

Oliveira

Lima

Ketavong

et al. 2021

Sci Rep

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“…These four species were mildly to poorly active on both assays (Table 3). Interestingly, while I. crithmoides was mostly ineffective in this study, its related species, I. viscosa 70% ethanolic extract exhibit anthelmintic properties against the larvae exsheathment of a mixture of Teladorsagia circumcincta and T. colubriformis parasites [32], suggesting signi cant chemical diversity among the genus.…”

Section: In Vitro Anthelmintic Propertiesmentioning

confidence: 71%

“…lentiscus) exhibited the highest activity on LEIA (IC 50 = 27.8-29.7 µg mL − 1 ) and egg hatching processes (IC 50 = 197.7 and 223.9 µg mL − 1 ), without signi cant differences between GIN species. Lentisk is an evergreen shrub with high polyphenol content and previous results have shown both in vitro and in vivo anthelmintic properties [29][30][31][32]. In previous studies, P. lentiscus extracts (acetone, ethanol and/or water) exhibited less than 20% larvae exsheathment and migration at 1200 µg mL − 1 [29,31].…”

Section: In Vitro Anthelmintic Propertiesmentioning

confidence: 92%

Disclosing the Bioactive Metabolites Involved in the in Vitro Anthelmintic Effects of Mediterranean Salt-Tolerant Plants: A Combined Approach Using PVPP and HPLC-ESI-MSn

Oliveira

Lima

Ketavong

et al. 2021

Preprint

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Strategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scaffolds on plants, yet salt tolerant plants remain overlooked. The aims of this study are to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt tolerant plant species and tested against Haemonchus contortus and Trichostrongylus colubriformis by the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus and Helychrisum italicum picardi were the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with PVPP, and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic effects were identified and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.

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“…Using a two-species but steady population of parasitic nematodes (ca. 20% Teladorsagia circumcinta and 80% Trichostrongylus colubriformis ) was used in different works since both are found in the same animal [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of selected Palestinian plants on the in vitro exsheathment of the third stage larvae of gastrointestinal nematodes

Jamous¹,

Ali‐Shtayeh²,

Abu-Zaitoun³

et al. 2017

BMC Vet Res

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BackgroundGastrointestinal parasites are one of the main restrictions to small ruminant production. Their pathological importance is primarily related to the major production losses, in quantity or quality, induced by the direct action of worms. Control of these parasites is based exclusively on the frequent use of anthelmintic drugs. However, the resistance to anthelmintics in worm populations after commercialisation of chemical drugs is now widespread. Therefore, there is a need to find new natural resources to ensure sustainable and effective treatment and control of these parasites. The aim of this study was to evaluate the anthelmintic activity, as minimum inhibitory concentration (IC 50 mg/mL), of different plant extracts using larval exsheathment inhibition assay using a two-species but steady population of parasitic nematodes (ca. 20% Teladorsagia circumcinta and 80% Trichostrongylus colubriformis).ResultsThe study showed that the ethanolic extracts of 22 out of the 48 plant extracts, obtained from 46 plant species, have an inhibitory effect >50% (at concentrations of 100 mg/mL) on the third stage larvae (L3) of the nematodes exhibited the strongest inhibition activity (94%) with IC 50 of 0.02 mg/mL, where other members of the Rhamnaceae family have shown to possess strong anthelmintic activity (70–89%).ConclusionsPlant extracts are potential rich resources of anthelmintics to combat helminthic diseases. Our results suggest that extracts from Rhamnus elaternus, Epilobium hirsutum, Leucaena leucocephala and Rhamnus palaestinus have promising anthelmintic activity, with potential applications in animal therapeutics and feed.

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Seasonal variation in the effects of Mediterranean plant extracts on the exsheathment kinetics of goat gastrointestinal nematode larvae

Cited by 12 publications

References 34 publications

Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

Disclosing the Bioactive Metabolites Involved in the in Vitro Anthelmintic Effects of Mediterranean Salt-Tolerant Plants: A Combined Approach Using PVPP and HPLC-ESI-MSn

Effects of selected Palestinian plants on the in vitro exsheathment of the third stage larvae of gastrointestinal nematodes

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