Seasonal variation in the essential oil composition and biological activities of Thymus linearis Benth. Collected from the Kumaun region of Uttarakhand, India

Kabdal, Tanuja; Himani,; Kumar, Ravendra; Prakash, Om; Nagarkoti, Kirti; Rawat, Deepa; Srivastava, Ranjan; Kumar, Satya; Dubey, Shiv Kumar

doi:10.1016/j.bse.2022.104449

Cited by 18 publications

(8 citation statements)

References 43 publications

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“…Interestingly, a phase I study regarding carvacrol showed clinical safety and tolerability in healthy subjects [ 46 ]. Moreover, potential antidiabetic activity has been reported for thymol extracted from several plant species: Thymus quinquecostatus , T. linearis , and T. serrulatus [ 47 , 48 , 49 ]. No previous studies have reported the possible activity of p -cymene.…”

Section: Discussionmentioning

confidence: 99%

Anti-Cholinesterase and Anti-α-Amylase Activities and Neuroprotective Effects of Carvacrol and p-Cymene and Their Effects on Hydrogen Peroxide Induced Stress in SH-SY5Y Cells

Caputo

Amato

Martino

et al. 2023

IJMS

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Several researchers have demonstrated the health and pharmacological properties of carvacrol and p-cymene, monoterpenes of aromatic plants. This study investigated these compounds’ possible anti-cholinesterase, anti-α-amylase, and neuroprotective effects. We evaluated the anti-acetylcholinesterase and anti-α-amylase activities at different concentrations of the compounds. The maximum non-toxic dose of carvacrol and p-cymene against SH-SY5Y neuroblastoma cells was determined using an MTT assay. The neuroprotective effects of the compounds were evaluated on H2O2-induced stress in SH-SY5Y cells, studying the expression of caspase-3 using Western blotting assays. Carvacrol showed inhibitory activities against acetylcholinesterase (IC50 = 3.8 µg/mL) and butyrylcholinesterase (IC50 = 32.7 µg/mL). Instead, the anti-α-amylase activity of carvacrol resulted in an IC50 value of 171.2 μg/mL After a pre-treatment with the maximum non-toxic dose of carvacrol and p-cymene, the expression of caspase-3 was reduced compared to cells treated with H2O2 alone. Carvacrol and p-cymene showed in vitro anti-enzymatic properties, and may act as neuroprotective agents against oxidative stress. Further studies are necessary to elucidate their possible use as coadjutants in preventing and treating AD in diabetic patients.

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Section: Discussionmentioning

confidence: 99%

Anti-Cholinesterase and Anti-α-Amylase Activities and Neuroprotective Effects of Carvacrol and p-Cymene and Their Effects on Hydrogen Peroxide Induced Stress in SH-SY5Y Cells

Caputo

Amato

Martino

et al. 2023

IJMS

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“…(1) In vitro Evaluation of Oleoresins Samples on Mortality of Second Stage Larvae of Meloidogyne incognita . [ 22 , 23 ]. To evaluate the impact of oleoresins on the mobility of second stage larvae of M. incognita , an in vitro experiment was conducted.…”

Section: Methodsmentioning

confidence: 99%

“…(2) In vitro Evaluation of GSAO and GSRO on Egg Hatching of Meloidogyne incognita . [ 23 , 24 ]. To determine the effectiveness of the oleoresins on the hatching of M. incognita eggs derived from nematode pure culture, an in vitro experiment was conducted.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Screening and Evaluation of Pesticidal Efficacy in the Oleoresins of Globba sessiliflora Sims and In Silico Study

Verma

Karakoti

Kumar

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Globba sessiliflora Sims is an aromatic rhizomatous herb of family Zingiberaceae which is endemic to Peninsular India. This study first reports the phytochemical profile and pesticidal potential of oleoresins obtained from the aerial and rhizome parts of Globba sessiliflora Sims. The oleoresins were prepared by the cold percolation method and were analyzed by a gas chromatography-mass spectrometry (GC-MS) method. Both the oleoresins varied greatly in composition, the major compounds identified in aerial part oleoresin (GSAO) were methyl linoleate, methyl palmitate, and phytol, while the major compounds present in rhizome part oleoresin (GSRO) were γ-sitosterol, 8 (17),12-labdadiene-15, 16-dial, methyl linoleate, and methyl palmitate. In order to evaluate the biological activities, the oleoresins were tested under laboratory conditions for nematicidal action and inhibition of egg hatching potential against root knot nematode, where GSRO was more effective. Insecticidal activity was performed against mustard aphid, Lipaphis erysimi and castor hairy caterpillar, Selepa celtis. In case of mustard aphid, GSRO (LC50 = 154.8 ppm) was more effective than GSAO (LC50 = 263.0 ppm), while GSAO (LC50 = 346.7.0 ppm) was more effective against castor hairy caterpillar than GSRO (LC50 = 398.1 ppm). The herbicidal activity was performed in the receptor species Raphanus raphanistrum subsp. sativus, and the oleoresins showed different intensities for seed germination inhibition and coleoptile and radical length inhibition. Molecular docking studies were conducted to screen the in vitro activities and through molecular docking, it was found that the major oleoresins components were able to interact with the binding pocket of HPPD and AChE with γ-sitosterol showing the best binding affinity.

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“…Different 3D complex structures for the target proteins for different pests were data mined from Protein Data Bank (PDB) viz. acetylcholinesterase protein (PDB ID: 1C2O) for M. incognita [29], anionic peroxidase (PDB ID: 1OG5) for R. raphanistrum [30] (PDB ID: 3QQQ) for S. celtis, and PDB ID: 5F3Y for L. erysimi have been taken directly from PDB (www.rscb.org/pdb) for validation of the biological activities tested. Thus, it is being reported for the first time.…”

Section: Molecular Docking Studiesmentioning

confidence: 99%

Comparative chemical composition and pesticidal evaluation of Acorus calamus accessions collected from different geographical locations

et al. 2023

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The objectives of the present study were to investigate the phytochemical composition of essential oils (EO) from rhizomes of Acorus calamus collected from Jorhat, Assam; Munsyari and Pantnagar, Uttarakhand, India. EOs were studied for different pesticidal activities viz; nematicidal, insecticidal, and herbicidal activity. To study the synergistic effect of EOs on pesticidal activity, four combinations of EOs were prepared. Phenylpropanoids with β-asarone as the main compound were identified in all collections with varying percentages. Its contribution was found to be 85.8% in Munsyari EOs followed by 74.3% in Pantnagar and 62.6% in Assam collections. All EOs exhibited dose-dependent in vitro nematicidal activity against Meloidogyne incognita in terms of immobility and inhibition of egg hatching. The activity was observed as maximum in the EO combination of all three collections (1:1:1) whereas minimum in the Assam collections. In insecticidal activity against Lipaphis erysimi and Selepa celtis, maximum mortality was observed in Munsyari collections. The oils were assessed for sprout inhibition activity in terms of seed germination inhibition, coleoptile growth of the shoot and root against Raphanus raphanistrum. Maximum seed germination inhibition, % shoot, and root growth inhibition were found in all collections EO combinations. To predict the possible mode of action and the structure-activity relationship between major compounds of EOs and biological activities, in silico molecular docking and ADME/Tox studies were performed. The docking results revealed the mode of action of proteins of insects, nematodes, and weeds and were found in support of in vitro experiments. The study may be helpful for the development of herbal-based pesticides after proper clinical trials.

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Seasonal variation in the essential oil composition and biological activities of Thymus linearis Benth. Collected from the Kumaun region of Uttarakhand, India

Cited by 18 publications

References 43 publications

Anti-Cholinesterase and Anti-α-Amylase Activities and Neuroprotective Effects of Carvacrol and p-Cymene and Their Effects on Hydrogen Peroxide Induced Stress in SH-SY5Y Cells

Anti-Cholinesterase and Anti-α-Amylase Activities and Neuroprotective Effects of Carvacrol and p-Cymene and Their Effects on Hydrogen Peroxide Induced Stress in SH-SY5Y Cells

Phytochemical Screening and Evaluation of Pesticidal Efficacy in the Oleoresins of Globba sessiliflora Sims and In Silico Study

Comparative chemical composition and pesticidal evaluation of Acorus calamus accessions collected from different geographical locations

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