Seasonal Variation of Bacteria in Sea Ice Contaminated by Diesel Fuel and Dispersed Crude Oil

Delille, Daniel; Bassères, Anne; Dessommes, A.

doi:10.1007/s002489900012

Cited by 54 publications

(17 citation statements)

References 51 publications

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“…The coincidence between high C18 mineralisation rate and the presence of high concentrations of C25+C27+C29+C31 alkanes in some reference and oiled bays of the Prince William Sound (60 30 0 N; 147 05 0 W) suggested that terrestrial biowaxes pre-conditioned the microbial populations for an efficient degradation of oil-derived alkanes (Sugai et al, 1997). A recent work conducted in Terre Adelie (Delille, Basse`res, & Dessommes, 1997) showed a significant response of the Antarctic sea-ice HDB community to the addition of oil and fertilizers to newly formed seaice. The contribution of HDB to the saprophytic communities passed from less than 0.1% to more than 95% in some sea-ice samples in 6 months in spite of the severe conditions of the Antarctic winter.…”

Section: Bacterial Activitymentioning

confidence: 95%

Crude oil bioremediation in sub-Antarctic intertidal sediments: chemistry and toxicity of oiled residues

Pelletier

Delille

2004

Marine Environmental Research

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100

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Section: Bacterial Activitymentioning

confidence: 95%

Crude oil bioremediation in sub-Antarctic intertidal sediments: chemistry and toxicity of oiled residues

Pelletier

Delille

2004

Marine Environmental Research

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100

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“…The prevalence for HC biodegradation exists in pristine cold environments, and alkane monooxygenases and hydrocarbonoclastic bacteria have been detected and characterized in both Arctic and Antarctic environments (Whyte et al 1996;Delille et al 1997;Fiala & Delille 1999;Whyte et al 2002;Yakimov et al 2003). Studies of oil-polluted Polar environments have shown abundance of Alphaproteobacteria, Gammaproteobacteria and Actinobacteria (Grossman et al 1999;Aislabie et al 2000, Juck et al 2000Yakimov et al 2003).…”

Section: Introductionmentioning

confidence: 97%

Biodegradation of Petroleum Hydrocarbons in Seawater at Low Temperatures (0–5 °C) and Bacterial Communities Associated with Degradation

2006

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In this study biodegradation of hydrocarbons in thin oil films was investigated in seawater at low temperatures, 0 and 5 degrees C. Heterotrophic (HM) or oil-degrading (ODM) microorganisms enriched at the two temperatures showed 16S rRNA sequence similarities to several bacteria of Arctic or Antarctic origin. Biodegradation experiments were conducted with a crude mineral oil immobilized as thin films on hydrophobic Fluortex adsorbents in nutrient-enriched or sterile seawater. Chemical and respirometric analysis of hydrocarbon depletion showed that naphthalene and other small aromatic hydrocarbons (HCs) were primarily biodegraded after dissolution to the water phase, while biodegradation of larger polyaromatic hydrocarbons (PAH) and C(10)-C(36) n-alkanes, including n-hexadecane, was associated primarily with the oil films. Biodegradation of PAH and n-alkanes was significant at both 0 and 5 degrees C, but was decreased for several compounds at the lower temperature. n-Hexadecane biodegradation at the two temperatures was comparable at the end of the experiments, but was delayed at 0 degree C. Investigations of bacterial communities in seawater and on adsorbents by PCR amplification of 16S rRNA gene fragments and DGGE analysis indicated that predominant bacteria in the seawater gradually adhered to the oil-coated adsorbents during biodegradation at both temperatures. Sequence analysis of most DGGE bands aligned to members of the phyla Proteobacteria (Gammaproteobacteria) or Bacteroidetes. Most sequences from experiments at 0 degree C revealed affiliations to members of Arctic or Antarctic consortia, while no such homology was detected for sequences from degradation experiment run at 5 degrees C. In conclusion, marine microbial communities from cold seawater have potentials for oil film HC degradation at temperatures< or =5 degrees C, and psychrotrophic or psychrophilic bacteria may play an important role during oil HC biodegradation in seawater close to freezing point.

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“…Only few studies have been performed on sea-ice [36,[38][39][40][41]. These studies address mainly the effect of the ice cover on oil degradation whereas Delille et al [42] investigated the impact of oil contamination on total and specific bacterial communities in Antarctic sea-ice using culture-dependent methods. This is the first study to assess changes in bacterial communities in Arctic sea-ice and surface melt pools as influenced by crude oil and it combines culture-based and culture-independent molecular methods.…”

Section: Introductionmentioning

confidence: 99%

Influence of crude oil on changes of bacterial communities in Arctic sea-ice

Gerdes

Brinkmeyer

Dieckmann

et al. 2005

FEMS Microbiology Ecology

148

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The danger of a petroleum hydrocarbon spillage in the polar, ice-covered regions is increasing due to oil exploration in Arctic offshore areas and a growing interest in using the Northern Sea Route (NSR) as an alternative transportation route for Arctic oil and gas. However, little is known about the potential impact of accidental oil spills on this environment. We investigated the impact of crude oil on microbial community composition in six different Arctic sea-ice samples incubated with crude oil at 1 degrees C in microcosms for one year. Alterations in the composition of bacterial communities were analyzed with the culture-independent molecular methods DGGE (denaturing gradient gel electrophoresis) and FISH (fluorescence in situ hybridization). DGGE, FISH and cultivation methods revealed a strong shift in community composition toward the gamma-proteobacteria in sea-ice and melt pool samples incubated with crude oil. Marinobacter spp., Shewanella spp. and Pseudomonas spp. were the predominant phylotypes in the oil-treated microcosms. The ability of indigenous sea-ice bacteria to degrade hydrocarbons at low temperature (1 degrees C) was tested using four representative strains cultivated from sea-ice enriched with crude oil. [14C]Hexadecane was degraded by the sea-ice isolates at 20-50% capacity of the mesophilic type strain Marinobacter hydrocarbonoclasticus, a known hydrocarbon degrader, incubated at 22 degrees C.

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Seasonal Variation of Bacteria in Sea Ice Contaminated by Diesel Fuel and Dispersed Crude Oil

Cited by 54 publications

References 51 publications

Crude oil bioremediation in sub-Antarctic intertidal sediments: chemistry and toxicity of oiled residues

Crude oil bioremediation in sub-Antarctic intertidal sediments: chemistry and toxicity of oiled residues

Biodegradation of Petroleum Hydrocarbons in Seawater at Low Temperatures (0–5 °C) and Bacterial Communities Associated with Degradation

Influence of crude oil on changes of bacterial communities in Arctic sea-ice

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