Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp (Cirrhina molitorella) and Nile tilapia (Oreochromis niloticus) in the tropical Xiangang Reservoir (Huizhou, China)

Cheng, WeiXuan; Liang, Xu‐Fang; Shen, Dan; Zhou, Qiong; He, Yan; He, Shan; Li, GuangYu

doi:10.1007/s11434-011-4871-7

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Oligonucleotides PCR primers for GST were developed based on the published primers sequences (Cheng et al, 2012) that were designed using the sequence from GenBank EU107284, while primers for GPx were designed using the Primer3 program (accessible at http://www.genome.wi.mit. edu/cgi-bin/primer/primer3www.cgi) on the base of its nucleotide sequences available on GenBank (GQ853451).…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

A multiparameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status

Mahfouz

Sherif²

2015

The Journal of Basic & Applied Zoology

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Aflatoxin is a common contaminant of foods, particularly in the staple diets of many developing countries. To evaluate adverse effects of aflatoxin B1 (AFB1) toxicity on health status in the Nile tilapia Oreochromis niloticus, fish were fed diet contaminated with either 20 or 100 ppb AFB1 for 6 or 12 weeks. Growth indices, survival rate and hepatosomatic index (HSI) were assessed. Blood samples were collected for hematological profiles (e.g. RBCs and WBC count, Hb content). Liver enzyme activity; aspartate aminotransferase (AST), alanine aminotransferase (ALT) as well as alkaline phosphatase (ALP), were evaluated and toxin residues in the liver and musculature were detected. Liver histopathological investigations were carried out, whereas antioxidant glutathione peroxidase (GPx) and glutathione S-transferase (GST) gene expression were determined in this tissue by semi-quantitative RT-PCR. Furthermore, to test the fish immune status, challenge against Aeromonas hydrophila was conducted. Results indicated that 100 ppb AFB1 negatively impacted O. niloticus weight gain, feed efficiency, hematological profiles, HSI as well as liver histopathology, while increase in AST, ALT, ALP liver enzymes activity was evidenced. Further, the expression of liver GPx and GST down-regulated and AFB1 residues were always detected in the liver and only in the musculature in fish fed 100 ppb AFB1 for 12 weeks. The ability of fish to withstand A. hydrophila infection was remarkably lowered. Overall, the results herein demonstrate the toxic effects of AFB1 in O. niloticus. The observed alterations in fish status, especially in the liver coincide well with the expected oxidative stress resulting from the AFB1 toxicity. ª 2015 The Egyptian German Society for Zoology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

A multiparameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status

Mahfouz

Sherif²

2015

The Journal of Basic & Applied Zoology

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“…Diet, lifestyle, and body structure influence the distribution of substances absorbed by aquacultured organisms (Cheng et al, 2012;. C. molitorella is a bottom-dwelling fish that consumes organisms and organic matter at the bottom of the water column (Mao et al, 1985;Yin et al, 2003;Huang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Distributions of and Correlations between Cd, Cr, and Hg Concentrations in Suspended Particles and Sediment in Aquaculture Ponds and in Cirrhinus molitorella Tissues

Lv¹,

Gan²,

Ruan³

et al. 2020

PJZ

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Effect of hexavalent chromium exposure on the liver and kidney tissues related to the expression of CYP450 and GST genes of Oreochromis niloticus fish: Role of curcumin supplemented diet

Mohamed

El-Houseiny

El-Murr

et al. 2020

Ecotoxicology and Environmental Safety

132

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Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp (Cirrhina molitorella) and Nile tilapia (Oreochromis niloticus) in the tropical Xiangang Reservoir (Huizhou, China)

Cited by 3 publications

References 36 publications

A multiparameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status

A multiparameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status

Distributions of and Correlations between Cd, Cr, and Hg Concentrations in Suspended Particles and Sediment in Aquaculture Ponds and in Cirrhinus molitorella Tissues

Effect of hexavalent chromium exposure on the liver and kidney tissues related to the expression of CYP450 and GST genes of Oreochromis niloticus fish: Role of curcumin supplemented diet

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