Seasonal variation of <i>Pseudomonas aeruginosa</i> in culture positive otitis externa in South East England

Villedieu, A.; Papesh, E.; Weinberg, S.E.; Teare, Louise; Radhakrishnan, Jayachandran; Elamin, Wael

doi:10.1017/s0950268818001899

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…Harvesting the samples and freezing them in À728C until thawing for DNA extraction functioned expectedly, as most samples yielded sufficiently data and had low proportion of suspected contaminants. There is also likely to be some seasonal variation in the microbial community, and earlier studies have shown such variation in acute otitis externa (42). Unfortunately, the seasonal variation of the healthy EAC microbiota could not be confirmed in this study.…”

Section: Discussionmentioning

confidence: 57%

Microbiome of the Healthy External Auditory Canal

Sjövall

Aho

Hyyrynen

et al. 2020

Otology &Amp; Neurotology

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Objective: To investigate the microbiota of the healthy external auditory canal (EAC) culture-independently and to evaluate the usefulness of the swabbing method in collecting EAC microbiota samples. Study Design: Cohort study. Patients: Fifty healthy asymptomatic working-age volunteers. Intervention: Samples were harvested with DNA-free swabs from the volunteers' EACs. Main Outcome Measures: Amplicon sequencing of the 16S rRNA gene was used to characterize the microbial communities in the samples. Results: The swabbing method is feasible for EAC microbiota sample collection. The analyzed 41 samples came from 27 female and 14 male subjects; 4 samples were excluded due to recent antimicrobial treatment and 5 because of low sequence count or suspected contaminant microbes. The four most frequent amplicon sequence variants in the microbiota data were Staphylococcus auricularis, Propionibacterium acnes, Alloiococcus otitis, and Turicella otitidis. Typically, the dominant amplicon sequence variant in a sample was one of the most frequent bacteria, but there were also subjects where the dominant species was not among the most frequent ones. The genus Alloiococcus was least common in females who reported cleaning their ears. Subjects with a high relative abundance of Alloiococcus typically had a low abundance of Staphylococcus, which may be a sign of the two being competing members of the microbial community. Conclusions: The most common bacteria in the microbiome of the healthy EAC were Staphylococcus auricularis, Propionibacterium acnes, Alloiococcus otitis, and Turicella otitidis. The EAC microbiota seems more diverse and individualized than previously thought. Also, ear cleaning habits seem to alter the EAC microbiome.

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Section: Discussionmentioning

confidence: 57%

Microbiome of the Healthy External Auditory Canal

Sjövall

Aho

Hyyrynen

et al. 2020

Otology &Amp; Neurotology

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“…The development of AOE infection is correlated with an alkaline shift of the normal acidic pH of the outer ear canal (10). The incidence of AOE also increases significantly during the summer, due to high temperatures and humidity (11) otitis externa, reported that infection was seen most frequently in August and September and least frequently in April and May (12). In our study, our cases of AOE occurred most frequently during the summer and least frequently during the spring, with July being the month with the highest number of AOE diagnoses (18 cases; 23.6%) and May being the month with the fewest (1 case; 1.3%).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of ciprofloxacin in the treatment of Acute Otitis Media

Mehel¹,

Turgut²,

ÇELİKER³

et al. 2022

J. Exp. Clin. Med.

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Acute otitis externa is an infection of the outer ear canal that can occur at any age and is diagnosed by symptoms and examination findings. The aims of our study were to understand the seasonal frequency of acute otitis externa and to determine the susceptibility of causative bacteria to ciprofloxacin. Discharge samples were collected from the external ear canals of 76 patients diagnosed with acute otitis externa. The samples were evaluated and culture-antibiogram results were compiled. Routine microbiological methods were used for the reproduction of clinical material as bacterial and fungal cultures. The 76 enrolled patients were 18–80 years old (mean 41.6 years). Acute otitis externa occurred most frequently during summer, especially in July. Culture results revealed Pseudomonas aeruginosa growth in 30 cases (39.4%), Staphylococcus aureus in 18 cases (23.6%), and Serratia spp. in 5 cases (6.5%), while fungal growth was observed in 10 cases (13.1%). The antibiogram analyses determined ciprofloxacin resistance in P. aeruginosa in 13 patients (43.3%) and in S. aureus in 6 patients (33.3%). Acute otitis externa should be treated with antibiotics in accordance with culture antibiogram results, while keeping in mind that bacterial causes could be ciprofloxacin resistant.

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“…As a result, a correlation between the development of otitis and swimming in water contaminated with the bacteria P. aeruginosa has been recorded [11,12]. There is also proof that otitis is directly connected with the presence of moisture [13]. There are factors that boost the rapid growth of P. aeruginosa.…”

Section: Introductionmentioning

confidence: 94%

Risk Analysis of Otitis Externa (Swimmer’s Ear) in Children Pool Swimmers: A Case Study from Greece

Pantazidou

Dimitrakopoulou

Kotsalou

et al. 2022

Water

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Otitis is an ear inflammation characterized by an accumulation of polluted fluids in the ear, inflating the drum, causing ear pain, and draining the mucous membrane (pus) into the ear canal if the drum is perforated. Swimmer’s otitis, also known as acute external otitis, is a medical condition that frequently affects competitive swimmers. The risk factor analysis study was based on data obtained between May 2018 and May 2019 from four public swimming pools in Patras, Achaia. A checklist was created to evaluate the pools’ operational conditions, and it included information on the pools’ sanitation as well as swimming pool hygiene guidelines. In addition, a questionnaire was devised to collect data on pool swimmers’ use of the pools. Microbiological testing of the pool water was done ahead of time, and data on external otitis cases from hospitals was gathered. Based on this information, a risk factor analysis was conducted. Gender, weight, and age do not appear to have an impact on the number of otitis media cases that occur because of swimming in the pools. There is also no statistically significant link between episodes and the frequency of otitis events in locker rooms, restrooms, or swimming pools. The frequency with which swimmers utilize the pool, rather than the pool’s microbial burden, is the most significant determinant in otitis episodes. Furthermore, there is no statistically significant link between chlorine odor and otitis episodes. Additionally, several abnormalities in the ear or the child’s history do not appear to affect otitis episodes. More research is needed to determine whether infections are linked to microbial load or if other factors are responsible for the emergence of waterborne infections.

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Seasonal variation of Pseudomonas aeruginosa in culture positive otitis externa in South East England

Cited by 12 publications

References 9 publications

Microbiome of the Healthy External Auditory Canal

Microbiome of the Healthy External Auditory Canal

Effectiveness of ciprofloxacin in the treatment of Acute Otitis Media

Risk Analysis of Otitis Externa (Swimmer’s Ear) in Children Pool Swimmers: A Case Study from Greece

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