Seasonal variation of microelement contents in leaves of Cyclocarea paliurus among the provenances

Abstract

Cyclocarya paliurus as a multiple function plant can accumulate biologically important microelement elements. To reveal the variation of selected microelement concentrations in leaves of C. paliurus provenances during the growing season, 12 C. paliurus provenances in the field trial were sampled five times at approximately 1-month intervals. The method of inductively coupled plasma optical emission spectrometer (ICP-OES) was employed to determinate average concentrations of Fe, Mn, Zn, Cu and Se in leaves of 12 C. paliurus provenances. The results show that on average, the concentrations of five microelement in the leaves follows an order of Fe > Mn > Zn > Cu > Se. Variance analysis shows that there are significant differences in Fe, Mn and Zn concentrations among the twelve provenances (p<0.05), while there is no significant difference between Cu and Se concentrations. A significant difference was also observed in the concentrations of five microelements at the different sampling times (p<0.001), but the mean concentrations for each microelement showed different temporal dynamic patterns. Meanwhile, a significant correlation between concentrations of Se and other measured microelements was detected in the leaves of C. paliurus, except for Mn.Obtained results not only demonstrated that leaves of C. paliurus exhibited higher levels of microelements (Fe, Mn, Cu, Zn and Se), but also provided a basis for breeding strategies of superior provenances with rich content of microelements, and choosing optimum harvesting time for

The effect of geographic variation on chemical composition, antioxidant and hypoglycemic activities of Morus alba L. polysaccharides

“…The highest content was determined in XJP with 4.13 ± 0.04%, while the lowest was observed in SDP with 1.07 ± 0.07%. Many researchers have found that the content of bioactive compounds from plants varied significantly in different production areas (Fang, Chu, et al, ; Fang, Yang, et al, ). The essential oils of Pinus halepensis varied significantly in 10 production areas (Djerrad, Kadik, & Djouahri, ).…”

Simultaneous Determination of Flavonoids and Triterpenoids in Cyclocarya Paliurus Leaves Using High-Performance Liquid Chromatography

“…Seven marker compounds in C. paliurus were positively identified by comparing the retention time, UV spectra and MS fragmentation with the corresponding data obtained from the analysis of reference compounds (Figure 3 (b)). In the previous studies, only isoquercitrin, kaempferol and quercetin have been used as marker compounds of C. paliurus (Fang et al, 2011;Yang et al, 2009;Xie et al, 2003). Although kaempferol-3-O-α-L-rhamnopyranoside , kaempferol-3-O-β-glucuronide (Zhang et al, 2005), arjunolic acid , cyclocaric acid B (Zhang et al, 2005) and pterocaryoside B (Cui and Li, 2012) have been identified as some compounds in C. paliurus, there are no reports on these compounds as markers in this plant.…”

“…To ensure the efficacy and safety of TCMs, qualitative and quantitative analysis of more major bioactive components is very important (Peng et al, 2013). The previous studies of C. paliurus have focused only on the quantification of total flavonoids (Mi et al, 2009;Chen et al, 2009;Jin et al, 2007) or a few selected flavonoids (kaempferol, quercetin and isoquercitrin) (Fang et al, 2011;Yang et al,2009;Xie et al, 2003) because many marker compounds are not commercially available, especially some characteristic compounds. The purposes of this study were to develop an effective method to extract the overall compounds and develop a simple HPLC analytical method to quantify seven major bioactive components (isoquercitrin, kaempferol-3-O-α-L-rhamnopyranoside, kaempferol-3-O-β-glucuronide, arjunolic acid, cyclocaric acid B, pterocaryoside B and pterocaryoside A) simultaneously.…”