Seasonal variations in fatty acid composition of pasture forage plants and CLA content in ewe milk fat

Meľuchová, B.; Blaško, Jaroslav; Kubinec, Róbert; Górová, Renáta; Dubravska, J.; Margetín, M.; Soják, Ladislav

doi:10.1016/j.smallrumres.2008.05.001

Cited by 100 publications

(94 citation statements)

References 20 publications

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“…The fatty acid composition in milk fat of the plant oil supplemented ewes was similar to that of the ewes grazed on a lowland pasture in the middle of a pasture season (Meluchova et al 2008), however, the content of unhealthy trans-10 18:1 was significantly higher. Optimization of the amount and fatty acid profile of the supplemented plant oils as well as the composition of winter basal ration can further improve milk fatty acid composition of ewes fed a winter diet.…”

Section: Resultsmentioning

confidence: 57%

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Soják¹,

Blaško²,

Kubinec³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The effects of adding sunflower and linseed oils to a standard winter ration with a lower concentrate content on the milk fatty acid composition in lactating ewes were investigated. Eighteen dairy ewes randomly chosen from the ewe flock were divided into three groups: the first group was fed a winter ration, the second one a winter ration supplemented with sunflower oil, and the third a winter ration supplemented with linseed oil for a period of 12 days. In the treatment groups, the concentrate was partially replaced by 3.0 g/100 g sunflower or linseed oils. Milk samples were taken daily from morning milking for the analysis of fatty acid composition to determine their temporal daily variations. The responses to sunflower oil compared with linseed oil addition after the end of experiment were slightly higher for conjugated linoleic acid (CLA) (P < 0.05), linoleic acid (P < 0.001), trans-10 18:1 (P < 0.001), 6:0 to 16:0 (P ≈ 0.05 -P < 0.001), whereas a higher content of α-linolenic acid (ALA) (3-fold) (P < 0.001), oleic acid (P < 0.001), and 18:0 (P < 0.001) was found in milk after linseed oil addition. The responses to both oil additions were relatively sustainable with regard to CLA, trans-11 18:1 (vaccenic acid, VA), and ALA content after the last 6 days of supplementation. The winter ration supplementation with sunflower or linseed oil led to a 3-fold increase in CLA milk fat content (0.6-2.0 or 1.8 g/100 g fatty acid methyl esters (FAME), P < 0.001) and a 3-fold increase in VA milk fat content (1.2-3.8 or 4.1 g/100 g FAME, P < 0.001), however the content of trans-10 18:1 was 5-6-fold higher, compared with unsupplemented winter ration. Plant oil supplementation enhanced the total content of CLA, VA, ALA by 5.0 and 3.9 g/100 g FAME (P < 0.001) for linseed and sunflower oil supplementation, and decreased the total content of 12:0, 14:0, and 16:0 by 9.3 and 5.8 g/100 g FAME (P < 0.001) compared to winter diet, respectively.

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Section: Resultsmentioning

confidence: 57%

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Soják¹,

Blaško²,

Kubinec³

et al. 2015

CZECH J ANIM SCI

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“…Various authors have suggested that fresh grass promotes the synthesis of CLA through a greater activity of Δ9-desaturase in the udder (Khanal & Olson 2004;Nudda 2005;Meluchová et al 2008;Kim et al 2009). Moreover, the high concentrations of soluble fibre and fermentable sugars in fresh grass can create an environment in the gastrointestinal tract of ruminants, without lowering the pH, that is favourable for the growth of the bacteria responsible for synthesising CLA and the production of vaccenic acid (Dhiman et al 1999).…”

Section: Resultsmentioning

confidence: 99%

“…Several components may be responsible, although to date investigations have revealed that two do have biological activity: namely, cis9, trans11 CLA and trans10, cis12 CLA (López Bote et al 2004;Bondia-Pons et al 2007). The cis9, trans11 isomer stimulates the growth, reduces the severity of diabetes, strengthens bones, decreases cholesterol levels, and has antiatherogenic properties (Jung et al 2006;Bondia-Pons et al 2007;Silva Hernández et al 2007;Meluchová et al 2008). In contrast, the trans10, cis12 isomer elicits a reduction in fat levels (Park et al 2007).…”

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confidence: 99%

Conjugated linoleic acid contents in cheeses of different compositions during six months of ripenin

Lobos-Ortega¹,

Revilla²,

González-Martín³

et al. 2012

Czech J. Food Sci.

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Lobos-Ortega I., Revilla I., González-Martín M.I., Hernández-Hierro J.M., Vivar-Quintana A., González-Pérez G. (2012): Conjugated linoleic acid contents in cheeses of different compositions during six months of ripening. Czech J. Food Sci., 30: 221-227.The study deals with the effects of the origin of milk (cow, ewe, goat, at different proportions), seasonality, and ripening time on the contents of conjugated linoleic acid (CLA) in 224 samples of cheese. The sum of the cis9, trans11 and trans10, cis12 isomers was determined by GC-FID, after the extraction and methylation of the fatty acids of the samples, observing that the mean amount of CLA was 2.22, 2.72, and 3.54 mg/g of cheese, depending on the proportions of cow's, goat's, or ewe's milks, respectively. The contents in cow's, ewe's, and goat's milk, together with the ripening time and seasonality, were seen to have significant effects (P < 0.05) on the concentration of CLA. The Pearson correlation revealed an inverse correlation between the content of CLA and the % of cow's milk (r = -0.269, P < 0.01) and seasonality (r = -0.290, P < 0.01), and a direct correlation between CLA content and the % of ewe's milk (r = 0.312, P < 0.01) and the month of ripening (r = 0.188, P < 0.01).

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“…The following fixed effects were included in the model: H i 5 herd (four levels, not considered in the analysis of FA composition traits because all individuals belonged to the same herd); OL j 5 ordinal number of lactation; NK k : number of kids born; ME l 5 ME1 genotype; LACT m 5 month of lactation. Season of sampling was included as an additional fixed factor in the analysis of milk FA traits because there is a seasonal effect on lipid composition of plant pastures and this might influence milk FA composition (Mel'uchová et al, 2008). Finally, LogSCC was used as a covariate in the analysis of milk FA traits because Jensen (2002) reported that udder health might have an impact on milk FA composition.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of the caprine malic enzyme 1 (ME1) gene and its association with milk quality traits in Murciano–Granadina goats

Zidi

Serradilla

Jordana

et al. 2010

Animal

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Malic enzyme 1 plays a fundamental role in lipid metabolism because it yields a significant amount of the NADPH necessary for fatty acid biosynthesis. In ruminants, however, its contribution to this biochemical process seems to be less relevant than in other livestock species. In this study, we have partially sequenced the goat ME1 gene with the aim of identifying polymorphic sites. Structural analysis of the goat ME1 amino acid partial sequence evidenced the existence of two dinucleotide-binding motifs, located at positions 158 to 163 (GLGDLG) and 301 to 306 (GAGEAA), and three amino acid residues (E245, D246 and D269) involved in the binding of Mn 21 and strongly conserved among mammalian species. Moreover, multiple sequence alignment allowed us to identify four single nucleotide polymorphisms at exons 5 (c.483C . T), 6 (c.667G . A), 9 (c.927C . T) and 11 (c.1200G . A). The effects of ME1 genotype on milk production and composition traits were investigated in a MurcianoGranadina goat population. None of the associations found reached statistical significance after applying Benjamini-Hochberg correction. At most, associations with uncorrected P-values below 0.01 were observed for C16:0, C18:1n-9t and total conjugated linoleic acids. These negative results reinforce the notion that ME1 plays an ancillary role in ruminant lipogenesis.

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Seasonal variations in fatty acid composition of pasture forage plants and CLA content in ewe milk fat

Cited by 100 publications

References 20 publications

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oils

Conjugated linoleic acid contents in cheeses of different compositions during six months of ripenin

Polymorphism of the caprine malic enzyme 1 (ME1) gene and its association with milk quality traits in Murciano–Granadina goats

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