Probiotics are live cultures of microorganisms administered orally and acted beneficially on host health. The addition of probiotics to the diet of poultry has been found to improve growth performance, feed conversion efficiency, immune responses and help in combating enteric pathogens. Therefore, this study was carried out to determine the role of probiotics for preventing Salmonella Enteritidis infection and its effect on the performance as well as the immune response of broiler chickens. The studied Salmonella Enteritidis isolate was isolated from chickens from Qena Provence, Upper Egypt. One hundred and thirty, one day old Ross broiler chicks were divided into four equal groups; the first group (G1) was fed on a balanced ration and considered a negative control group.The second group (G2) was fed on a balanced ration and provided with the probiotic (Micro-Procell, cheil-Bio.com. LTD) containing Lactobacillus Plantarum 1×10 8 cfu, Lactobacillus Acidophilus 1×10 8 cfu and Saccharomyces Cerevisiae1×10 7 cfu, in drinking water for 5 successive days. The third group (G3) was challenged with Salmonella Enteritidis 10 9 Cfu / ml after Probiotics treatment and the fourth group (G4) was challenged with Salmonella Enteritidis 10 9 Cfu / ml at 6 days old and considered the positive control group. All groups were kept under complete observation for 4 weeks. Throughout the time of the experiment, both clinical signs and post mortum lesions were recorded for all groups, body weight (BW), food conversion ratio (FCR), total bacterial count, differential leucocytic count, phagocytic activity, serum biochemical parameters and humoral immunity (IgG and IgM) using ELISA technique were investigated. Results revealed high performance parameters, as an increase in body weight and FCR. Neither clinical signs nor PM appeared in both non infected group and the probiotics treated groups. The infected, probiotic treated group showed mild decrease in the performance parameters and mild degree of clinical sings and PM lesions for Salmonella Enteritidis infections. While the infected non probiotics treated group showed significant decrease in body weight, low of the performance parameters and characteristics sings and pm lesions for Salmonella Enteritidis infections along the experiment. Total bacterial count were decreased in infected treated group than infected one, differential leucocytic count showed increase monocyte and lymphocyte in propiotic treated group also the immune status assessment clarified that both phagocytic percentage and index significantly increased (P≤0.