Seasonal variations of physiological and cellular biomarkers and their use in the biomonitoring of north adriatic coastal waters (Croatia)

Petrović, Siniša; Semenčić, Lorena; Ozretić, Bartolo; Ozretić, Mirjana

doi:10.1016/j.marpolbul.2004.05.004

Cited by 69 publications

(46 citation statements)

References 22 publications

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“…The fact that LP was always under reference values (20 to 25 min) in both localities could explain the lack of a seasonal variability pattern. Indeed, a seasonal pattern in lysosomal membrane stability has been reported in hemocytes (Bocchetti & Regoli 2006) and digestive cells (Petrović et al 2004) of mussels from the Adriatic Sea and in digestive cells of mussels from the Basque Coast (Izagirre et al 2008), although in a study carried out in mussels from the Mediterranean Sea LP was not significantly different between May and October (Zorita et al 2007). Thus the lack of seasonal variability observed in the present study might be indicative of persistence of the POS effects on lysosomal membrane integrity beyond those recorded for 2 to 3 yr after the spill , although LP values tend to increase up to values indicative of a healthy status after longterm effects (Fig.…”

Section: Discussionmentioning

confidence: 90%

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Garmendia¹,

Soto²,

Cajaraville³

et al. 2010

Aquat. Biol.

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Mussels Mytilus galloprovincialis were collected monthly in 2 localities in the Northern Iberian Peninsula, Oia (Galicia) and Mundaka (Basque Coast), from April 2005 to May 2006, to investigate whether seasonal variability in biomarkers follows the same pattern at both geographical areas. The following battery of biomarkers was analysed: lysosomal membrane stability and structural changes, intracellular neutral lipid accumulation, cell type composition in the digestive gland epithelium, structural changes of digestive alveoli and digestive gland histopathology. Additionally, gonad index was measured as a supporting parameter. Overall, results demonstrate that most studied biomarkers showed similar seasonal variability pattern in both localities. Lysosomal membrane stability (labilisation period [LP]) and most histopathological conditions showed similar seasonal variability patterns in both localities, although values of LP and prevalences of some parasites were higher in Mundaka. Data provide baseline values to be used as reference values for the biomonitoring programmes carried out in coastal areas from Galicia to the Basque Coast. It is concluded that for monitoring programmes based on biomarkers, samples must be taken at least twice per year (spring and autumn) to avoid the effect on biomarkers of the late stages of each reproductive cycle.

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Section: Discussionmentioning

confidence: 90%

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Garmendia¹,

Soto²,

Cajaraville³

et al. 2010

Aquat. Biol.

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show abstract

“…Tremblay et al (1998) also found that LP values are lower in summer than in winter in mussel digestive cells. In mussels from the Adriatic coast, however, lysosomal membrane stability is less season-dependent than other biomarkers, even though stability is somehow reduced in summer (Petrovic et al 2004). Fig.…”

Section: Seasonmentioning

confidence: 89%

“…In mussels from temperate regions, molecular and cellular processes are known to present a clear pattern of seasonal variability (Cancio et al 1999, Manduzio et al 2004, Petrovic et al 2004, Ivankovic et al 2005) that has been related to seasonal changes in temperature, food availability and gonad development (Etxeberria et al 1995, Leiniö & Lehtonen 2005. Along the Basque coast, the seasonal pattern of variation in water temperature and food availability is well known (Madariaga & Orive 1989, Orive et al 2004.…”

Section: Seasonmentioning

confidence: 99%

“…Gonad development (Cáceres-Martínez & Figueras 1998), food composition and food availability (Wong & Cheung 2003) are believed to be major factors governing seasonality in mussels (Etxeberria et al 1995, Leiniö & Lehtonen 2005. The amplitude of seasonal variability may differ between species (Leiniö & Lehtonen 2005) and between localities (Petrovic et al 2004), and it has been suggested that it is largely attenuated in stressed populations (Marigómez et al 1996). Accordingly, lysosomal membrane stability in mussel digestive gland was highly variable through the year in a clean site whereas in a polluted site values were lower and more constant between seasons (Regoli 1992).…”

Section: Introductionmentioning

confidence: 99%

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Natural variability in size and membrane stability of lysosomes in mussel digestive cells: seasonal and tidal zonation

Izagirre¹,

Ramos²,

Marigómez

2008

Mar. Ecol. Prog. Ser.

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Lysosomal responses in mussel digestive cells are widely accepted as effective biomarkers that respond to pollutant exposure, as well as to natural factors (tide, food, temperature). The effects of tide and season on digestive cell lysosomes in mussels were determined in different transect and transplant experiments on mussels at different tide levels and in winter and summer. We measured lysosomal enlargement through the lysosomal structural changes (LSC) test after β-glucuronidase (β-Gus) histochemistry and membrane destabilisation through the lysosomal membrane stability (LMS) test after hexosaminidase (Hex) histochemistry. A marked gradient along the intertidal zone, which was more prominent in winter than in summer, was found in lysosomal size and membrane stability. A transplant experiment revealed that digestive cell lysosomes respond rapidly to immersion of mussels. The study of the time-course changes in mussels from high tidemark level indicated that lysosomes respond very promptly to air exposure conditions. We suggest that the lysosomal changes observed are the result of the digestive process in parallel with tide. Since such natural variability might mask the effects of pollution, we conclude that a standardised sampling protocol -which should include the use of mussels from low intertidal levels -is needed.

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“…This is most apparent in the consumption data where 18% of PC shrimp failed to eat. A lowered ability to tolerate stress has been correlated with exposure of shrimp to contaminants [23], and the movement to the laboratory represents a stress that a significant amount of PC shrimp could not tolerate.…”

Section: Discussionmentioning

confidence: 99%

Compensatory partitioning of carbon budgets by the grass shrimp (Palaemonetes pugio) and implications for predator prey relationships

Stout

Taghon

2013

Open Life Sciences

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Carbon utilization and allocation were examined between two populations of shrimp (Palaemonetes pugio) to determine the possible effects of living in an area of high anthropogenic impact. Carbon assimilation has been studied in P. pugio, but no study has looked at how assimilation might be influenced by contaminants. Anthropogenic effects on carbon assimilation in grass shrimp represent a major unmeasured impact on the carbon budget of multi-cellular organisms in estuaries and near shore environments. The influence of anthropogenic contamination on carbon assimilation has implications for predicting the environmental impact of contaminants, for models of estuarine function, and trophic transfer from the dominant macroscopic detritus processor to species of direct economic importance. Shrimp budgets were compared between two populations, one from a highly polluted marsh creek system in Northern New Jersey, and one from a clean reference site in Southern New Jersey. All components of the carbon budgets were measured directly including carbon allocated to reproduction. Carbon lost to respiration was lower in shrimp from the polluted system allowing them to have increased reproductive output. This is examined in the context of previous studies that show lowered predation by a piscine predator at the polluted site, resulting in a trophic cascade and changes in ecosystem function due to anthropogenic impacts.

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Seasonal variations of physiological and cellular biomarkers and their use in the biomonitoring of north adriatic coastal waters (Croatia)

Cited by 69 publications

References 22 publications

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Natural variability in size and membrane stability of lysosomes in mussel digestive cells: seasonal and tidal zonation

Compensatory partitioning of carbon budgets by the grass shrimp (Palaemonetes pugio) and implications for predator prey relationships

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