Seasonality and mycorrhizal colonization in three species of epiphytic orchids in southeast Mexico

Bertolini, Vincenzo; Cruz-Blasi, Jesús; Damon, Anne; Mora, Javier Valle

doi:10.1590/0102-33062014abb3436

Cited by 18 publications

(13 citation statements)

References 16 publications

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“…The percentage of fungal colonization in orchid roots also varies according to the root types. Bertolini et al (2014) demonstrated that mycorrhization is meticulously extant in orchid roots and it is dependent on the wet and dry seasons.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Endophytic Fungi from the Roots of two epiphytic orchids: Rhynchostylis retusa (L.) Bl. and Vanda tessellata (L.) Bl

Sayem,

Miah,

Hossain

2024

Preprint

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Two fungal endophytes were isolated and identified from two indigenous orchids of Bangladesh namely, Rhynchostylis retusa (L.) Bl. and Vanda tessellata (L.) Bl. Nature of fungal colonization, seasonal variations of colonization in root cortex cells were also studied. The fungal endophytes isolated from two different orchids differed in their cultural morphology and microscopic features such as colony morphology, colour of the colony, presence/absence of monilioid cells or spores and diameter of vegetative hyphae. The microscopic features i.e., hyphal structure, right-angle branching of hyphae, slight constriction at the branching point, shape and diameter of monilioid cells of the endophytic fungus isolated from R. retusa showed resemblance with the anamorphic Rhizoctonia-like fungi Ceratobasidium sp. On the other hand, the fungal endophytes isolated from the roots of V. tessellata produced huge number of spores. The cultural characteristics and spore morphology of this fungi corroborated resemblance with pathogenic fungi, Fusarium sp. The identity of the fungi was further reconfirmed through sequencing of the internal transcribed spacer (ITS) of the nuclear ribosomal DNA (nrDNA). The BLASTn search of ITS region sequences of the endophytic fungi isolated from R. retusa exhibited maximum similarity (97%) with an orchid mycorrhizal fungi (OMF), Ceratobasidium papillatum (GeneBank Accession No. OQ073691), while the fungal endophyte isolated from V. tessellata showed maximum similarity (99%) with Fusarium ambrosium (GeneBank Accession No. OP752102). The phylogenetic tree constructed using ITS region sequences of the isolated fungal endophytes and their closely linked species from genebank data developed two distinct groups. The conventional and molecular approaches applied for identification of these OMF can be followed for easy and accurate identification of other OMF.

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Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Endophytic Fungi from the Roots of two epiphytic orchids: Rhynchostylis retusa (L.) Bl. and Vanda tessellata (L.) Bl

Sayem,

Miah,

Hossain

2024

Preprint

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“…Transversal root segments of approximately 2 mm were cut and sterilized by serial ethanol baths (Otero & Bayman 2009. Roots segments were examined for the presence of pelotons with a dissecting microscope at a 4X magnification, measuring the % of the total root section occupied by fungi following previously published methods (Bertolini et al 2014). Root sections that showed presence of hyphae coils (pelotons) in their cells were placed in Petri dishes with autoclaved potato dextrose agar (PDA) medium at pH 5.8.…”

Section: Field Sampling and Morphological Identification Of The Fungamentioning

confidence: 99%

Mycorrhizal fungi associated with Codonorchis lessonii (Brongn.) Lindl., a terrestrial orchid from Chile

Pereira¹,

Suz

Albornoz³

et al. 2018

Gayana Bot.

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Almost all orchid species depend on association with fungal partners. These fungal species facilitate orchid seed germination and promote growth and possibly stress tolerance in adult plants, both in the field and laboratory conditions. Codonorchis lessonii is a terrestrial orchid, endemic to southern Chile and Argentina with a currently unknown conservation status. Previous studies have reported Rhizoctonia-like fungi associated with C. lessonii in Argentina, but their fungal partners in Chilean populations are unknown. This study aims to characterize and isolate the mycorrhizal fungi associated with adult individuals of C. lessonii from three different populations in Central-South Chile. Root sections showing pelotons were used for the isolation and identification of the fungal taxa. Radial fungal growth was measured for all mycelial isolates. The presence of binucleate cells placed all isolates within the families Ceratobasidiaceae and Tulasnellaceae and the identification was confirmed by molecular analysis. Fungal isolates belonging to Ceratobasidiaceae grew at a higher rate than those from Tulasnellaceae. Phylogenetic analyses showed that different fungal partners associate with this orchid, suggesting relatively low specificity. The isolation and identification of the fungal partners of C. lessonii could help understand its ecology and contribute in future restoration and propagation initiatives for the species.

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“…Many species in the Orchidaceae family are terrestrial and have subterranean roots or tubers, while over 70% of the members of this family are epiphytic (including lithophytic) and their roots are usually exposed on the surface of rocks or tree stems [26]. Similar with the terrestrial orchids, the epiphytic species also have persistently present OMF colonization in their roots [27]. Different plant taxa and their mycorrhizal fungi may have various physiological demands (e.g., in water and nutrition utilization) [28].…”

Section: Introductionmentioning

confidence: 99%

Mycorrhizal Fungal Partners Remain Constant during a Root Lifecycle of Pleione bulbocodioides (Orchidaceae)

Qin

Feng

Zhang

et al. 2021

JoF

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Mycorrhizal mutualisms are vital for orchids through germination to adulthood. Fungal species diversity and community composition vary across seasons and plant development stages and affect plant survival, adaptation, and community maintenance. Knowledge of the temporal turnover of mycorrhizal fungi (OMF) remains poorly understood in the eco-physiologically diverse orchids (especially in epiphytic orchids), although it is important to understand the function and adaptation of mycorrhizae. Some species of Pleione are epiphytic plants with annual roots and may recruit different fungal partners during their root lifecycle. Based on continuous samplings of Pleione bulbocodioides during a whole root lifecycle, we characterized the fungal temporal dynamics using Illumina sequencing of the ITS2 region. Our data showed that the plants of P. bulbocodioides were quickly colonized by OMF at root emergence and had a constant OMF composition throughout one root lifecycle, although the OMF richness declined with root aging after a peak occurrence during root elongation. In contrast, the richness of root-inhabiting fungal endophytes kept increasing with root aging and more drastic turnovers were found in their species compositions. Our findings of OMF temporal turnover contribute to further understanding of mycorrhizal associations and adaptation of Orchidaceae and will benefit orchid resource conservation and utilization.

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Seasonality and mycorrhizal colonization in three species of epiphytic orchids in southeast Mexico

Cited by 18 publications

References 16 publications

Isolation and Identification of Endophytic Fungi from the Roots of two epiphytic orchids: Rhynchostylis retusa (L.) Bl. and Vanda tessellata (L.) Bl

Isolation and Identification of Endophytic Fungi from the Roots of two epiphytic orchids: Rhynchostylis retusa (L.) Bl. and Vanda tessellata (L.) Bl

Mycorrhizal fungi associated with Codonorchis lessonii (Brongn.) Lindl., a terrestrial orchid from Chile

Mycorrhizal Fungal Partners Remain Constant during a Root Lifecycle of Pleione bulbocodioides (Orchidaceae)

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