Seasonality in expression and distribution of nitric oxide synthase isoforms in the testis of the catfish, Clarias batrachus: Role of nitric oxide in testosterone production

Existence of a nitric oxide synthase/nitric oxide system in fish testis and its role in modulation of androgenesis

“…Effects of exogenous NO donor (sodium nitroprusside-SNP), a NOS inhibitor (N-nitro-L-arginine methyl ester-L-NAME), and macrophage-produced NO on testicular production in vivo or in vitro conditions were assessed by analyzing testosterone using ELISA kit. Details of methodology and various control groups for proper comparisons were published elsewhere (Dubey nee Pathak and Lal 2008, 2009, 2010.…”

Role of nitric oxide in motility and fertilizing ability of sperm of Heteropneustes fossilis (Bloch.)

“…Although Creech et al (1998) could not detect NOS activity in sperm but suggested the presence of a trace amount of NO by ESR signal in fathead minnow sperm with seminal plasma. Recent studies have also shown the existence of nNOS and iNOS in advanced germ cells in fish testis (nee Pathak and Lal, 2010) indirectly supporting the hypothesis that fish sperm are capable of generating NO. The presence of NO in sperm has convincingly been demonstrated in a number of mammals (Lewis et al, 1996;Herrero et al, 2000;Goud et al, 2008).…”