Seasonality of Post-capture Longevity in a Medically-Important Mosquito (Culex pipiens)

Papadopoulos, Nikos T.; Carey, James R.; Ioannou, Charalampos S.; Ji, Hao; Müller, Hans‐Georg; Wang, Jane-Ling; Luckhart, Shirley; Lewis, Edwin E.

doi:10.3389/fevo.2016.00063

Cited by 18 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pipiens f. molestus females (Tables 3 & 5). This observation is in accordance with a previous study documented that the physiological changes that take place during the pre-hibernation transition of these females confer a considerable increase in their longevity potential [50].…”

Section: Discussionsupporting

confidence: 94%

Resistance Development to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis of Culex Pipiens f. Pipiens and Culex Pipiens f. Molestus (Diptera: Culicidae) and its Effects on Their Winter Survival

Ioannou¹,

Hadjichristodoulou²,

Kyritsi³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The Culex pipiens mosquito consists of two forms named pipiens and molestus that exhibit substantial differences in their biology including overwintering behavior. Diflubenzuron (DFB) and Bacillus thuringiensis subsp. israelensis (Bti) are among the most widely used larvicides for controlling Cx. pipiens populations. The high dependency on these two larvicides, pose major concerns for resistance development. The evolution and stability of resistance to insecticides has been associated with fitness costs that may be manifested under stressful conditions such as the winter period. The aim of the present study was to explore the resistance development of pipiens and molestus forms to both larvicides and its potential fitness costs on their winter survival.Methods: Colonies of both forms of Cx. pipiens were established from the same area. Following the World Health Organization protocols (WHO), the efficacy of both larvicides was determined for each mosquito population. Then, larvae from each form were selected for three successive generations by applying fixed doses corresponding to IE80 (IE: Inhibition of adult Emergence) and LC80 (LC: Lethal Concentration) for DFB and Bti respectively. At the end of this process, the resistance levels and the winter survival of the selected populations relative to controls (colonies that received no selection) were determined.Results: Contrary to Bti, selection with DFB induced different levels of resistance between the two forms of Cx. pipiens. The selected populations of Cx. pipiens f. molestus to both larvicides exhibited a high fitness cost in terms of reduced winter larval survival rates relative to control. Moreover, the obtained adults of the Bti selected population experienced significantly shorter lifespan compared to control and DFB selected population. On the other hand, selection with both DFB and Bti had no apparent effects on Cx. pipiens f. pipiens female winter survival rates relative to control. Furthermore, the reproductive parameters and the longevity of the overwintered females were similar between the selected populations and the control. Conclusions: Our findings are expected to contribute on the better understanding of the resistance development and evolution of the two forms of Cx. pipiens to DFB and Bti allowing the adoption of appropriate resistance management strategies.

show abstract

Section: Discussionsupporting

confidence: 94%

Resistance Development to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis of Culex Pipiens f. Pipiens and Culex Pipiens f. Molestus (Diptera: Culicidae) and its Effects on Their Winter Survival

Ioannou¹,

Hadjichristodoulou²,

Kyritsi³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 94%

Short Term Selection to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis Differentially Affects the Winter Survival of Culex Pipiens F. Pipiens and Culex Pipiens F. Molestus (Diptera: Culicidae)

Ioannou

Hadjichristodoulou

Kyritsi

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe Culex pipiens mosquito consists of two forms named pipiens and molestus that exhibit substantial differences in their biology including overwintering behavior. Diflubenzuron (DFB) and Bacillus thuringiensis subsp. israelensis (Bti) are among the most widely used larvicides for controlling Cx. pipiens populations. The high dependency on these two larvicides, pose major concerns for resistance development. The evolution and stability of tolerance/resistance to insecticides has been associated with fitness costs that may be manifested under stressful conditions such as the winter period. This study aimed to investigate how short term selection of pipiens and molestus forms to both larvicides affect their susceptibility and their overwintering success.MethodsColonies of both forms of Cx. pipiens were established from the same area. Following standard protocols, the efficacy of both larvicides was determined for each mosquito population. Then, larvae from each form, were selected for three successive generations by applying fixed doses corresponding to IE80 (Inhibition of adult Emergence) and LC80 (Lethal Concentration) for DFB and Bti, respectively. At the end of this process, the susceptibility levels and the winter survival of the selected populations relative to controls (colonies that received no selection) were determined.ResultsContrary to Bti, selection with DFB induced different susceptibility levels between the two forms of Cx. pipiens. The selected populations of Cx. pipiens f. molestus to both larvicides exhibited a high fitness cost in terms of reduced winter larval survival rates relative to control. Moreover, the obtained adults of the Bti selected population experienced significantly shorter lifespan compared to control and DFB selected population. On the other hand, selection with both DFB and Bti had no apparent effects on Cx. pipiens f. pipiens female winter survival rates relative to control. Furthermore, the reproductive parameters and the longevity of the overwintered females were similar between the selected populations and the control. ConclusionsOur findings reveal that sort term selection to DFB and Bti induces a high fitness cost on the winter survival of Cx. pipiens f. molestus but not of pipiens form, suggesting potential differences on the persistence of tolerant individuals from year to year between the two forms.

show abstract

“…In contrast, we show that mosquito mortality rate is most accurately described using an age-dependent distribution, in this case, a Gompertz function. Other studies also suggest age-dependent survival (e.g., [ 40 , 62 – 65 ]). Here, we show that the age-dependence holds across temperatures, with initial senescence rate (α) increasing at higher temperatures.…”

Section: Discussionmentioning

confidence: 99%

Quantifying the effects of temperature on mosquito and parasite traits that determine the transmission potential of human malaria

2017

View full text Add to dashboard Cite

Malaria transmission is known to be strongly impacted by temperature. The current understanding of how temperature affects mosquito and parasite life history traits derives from a limited number of empirical studies. These studies, some dating back to the early part of last century, are often poorly controlled, have limited replication, explore a narrow range of temperatures, and use a mixture of parasite and mosquito species. Here, we use a single pairing of the Asian mosquito vector, An. stephensi and the human malaria parasite, P. falciparum to conduct a comprehensive evaluation of the thermal performance curves of a range of mosquito and parasite traits relevant to transmission. We show that biting rate, adult mortality rate, parasite development rate, and vector competence are temperature sensitive. Importantly, we find qualitative and quantitative differences to the assumed temperature-dependent relationships. To explore the overall implications of temperature for transmission, we first use a standard model of relative vectorial capacity. This approach suggests a temperature optimum for transmission of 29°C, with minimum and maximum temperatures of 12°C and 38°C, respectively. However, the robustness of the vectorial capacity approach is challenged by the fact that the empirical data violate several of the model’s simplifying assumptions. Accordingly, we present an alternative model of relative force of infection that better captures the observed biology of the vector–parasite interaction. This model suggests a temperature optimum for transmission of 26°C, with a minimum and maximum of 17°C and 35°C, respectively. The differences between the models lead to potentially divergent predictions for the potential impacts of current and future climate change on malaria transmission. The study provides a framework for more detailed, system-specific studies that are essential to develop an improved understanding on the effects of temperature on malaria transmission.

show abstract

Seasonality of Post-capture Longevity in a Medically-Important Mosquito (Culex pipiens)

Cited by 18 publications

References 34 publications

Resistance Development to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis of Culex Pipiens f. Pipiens and Culex Pipiens f. Molestus (Diptera: Culicidae) and its Effects on Their Winter Survival

Resistance Development to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis of Culex Pipiens f. Pipiens and Culex Pipiens f. Molestus (Diptera: Culicidae) and its Effects on Their Winter Survival

Short Term Selection to Diflubenzuron and Bacillus Thuringiensis Subsp. Israelensis Differentially Affects the Winter Survival of Culex Pipiens F. Pipiens and Culex Pipiens F. Molestus (Diptera: Culicidae)

Quantifying the effects of temperature on mosquito and parasite traits that determine the transmission potential of human malaria

Contact Info

Product

Resources

About