Seawater copper content controls biofilm bioaccumulation and microbial community on microplastics

Djaoudi, Kahina; Onrubia, Javier A. Tesán; Boukra, Amine; Guesnay, Lucas; Portas, Aurélie; Barry‐Martinet, Raphaëlle; Angeletti, Bernard; Mounier, Stéphane; Lenoble, Véronique; Briand, Jean‐François

doi:10.1016/j.scitotenv.2021.152278

Cited by 22 publications

(4 citation statements)

References 87 publications

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“…The microeukaryotic component of the plastisphere has been largely overlooked to date. Most metabarcoding studies targeting microeukaryotes have targeted the V4, V7, or V9 regions of the 18S rRNA gene and highlighted the occurrence of diatoms (Stramenopiles) as early colonizers, while high occurrence of dinoflagellates (Alveolata), algal species, and even metazoan taxa represent a significant fraction of the eukaryotic communities [26,[32][33][34][35][36]. Still, co-occurrence networking analysis suggests that microeukaryotes play a limited role in the mature biofilm compared to bacteria [34].…”

Section: Introductionmentioning

confidence: 99%

Fungal Diversity and Dynamics during Long-Term Immersion of Conventional and Biodegradable Plastics in the Marine Environment

et al. 2023

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Plastics are associated with a worldwide pollution crisis, with strong negative impacts on both terrestrial and aquatic ecosystems. In marine environments, various organisms are colonizing plastic debris, but few studies have focused on fungal communities despite their non-trivial ecological roles in the marine environment. In this study, different types of plastics (biodegradable and conventional) immersed in marine natural environments and under laboratory controlled settings were collected after long-term colonization. Using a metabarcoding approach targeting two genetic markers, namely, the ITS2 region and the V4 hypervariable region of the 18S rRNA gene, we highlighted that fungal communities associated with plastic polymers were distinct from those found in the surrounding seawater. They also differed significantly between sampling locations and the nature of immersed polymers, indicating that fungal colonization was impacted by the sites and types of plastics, with clear dissimilarities between conventional and biodegradable polymers. Specifically for the conventional PVC polymer (Polyvinyl chloride), we also observed the successive stages of biofilm development and maturation after long-term immersion in seawater. A noticeable change in the fungal communities was observed around 30–40 days in natural settings, suggesting a colonization dynamic likely associated with a transition from biofilm formation to distinct communities likely associated with biofouling. Overall, this study strengthens the idea that the fungal kingdom is an integrated part of the “plastisphere”.

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Section: Introductionmentioning

confidence: 99%

Fungal Diversity and Dynamics during Long-Term Immersion of Conventional and Biodegradable Plastics in the Marine Environment

et al. 2023

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“…The removal of these microplastics is a difficult task due to their very small size, buoyancy, and easy dispersion. However, it has been discovered that these microplastics allow for the formation of biofilms, which increases the coagulation and flocculation of these structures, facilitating their elimination [ 67 ]. Consequently, methods have been developed to trap microplastics using biofilms, as in the case of Liu and collaborators, who developed a method to capture and release microplastics as needed using wild-type and modified strains of P. aeruginosa [ 68 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Isolation of Phenol- and Thiol-Derived Epicatechin Adducts Prepared from Avocado Peel Procyanidins Using Centrifugal Partition Chromatography and the Evaluation of Their Antimicrobial and Antioxidant Activity

Berrios-Henríquez,

Venegas-Toloza,

Reyes-Fuentes

et al. 2024

Molecules

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Polyphenols from agro-food waste represent a valuable source of bioactive molecules that can be recovered to be used for their functional properties. Another option is to use them as starting material to generate molecules with new and better properties through semi-synthesis. A proanthocyanidin-rich (PACs) extract from avocado peels was used to prepare several semi-synthetic derivatives of epicatechin by acid cleavage in the presence of phenol and thiol nucleophiles. The adducts formed by this reaction were successfully purified using one-step centrifugal partition chromatography (CPC) and identified by chromatographic and spectroscopic methods. The nine derivatives showed a concentration-dependent free radical scavenging activity in the DPPH assay. All compounds were also tested against a panel of pathogenic bacterial strains formed by Listeria monocytogenes (ATCC 7644 and 19115), Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 11775 and 25922), and Salmonella enterica (ATCC 13076). In addition, adducts were tested against two no-pathogenic strains, Limosilactobacillus fermentum UCO-979C and Lacticaseibacillus rhamnosus UCO-25A. Overall, thiol-derived adducts displayed antimicrobial properties and, in some specific cases, inhibited biofilm formation, particularly in Listeria monocytogenes (ATCC 7644). Interestingly, phenolic adducts were inactive against all the strains and could not inhibit its biofilm formation. Moreover, depending on the structure, in specific cases, biofilm formation was strongly promoted. These findings contribute to demonstrating that CPC is a powerful tool to isolate new semi-synthetic molecules using avocado peels as starting material for PACc extraction. These compounds represent new lead molecules with antioxidant and antimicrobial activity.

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“…These deleterious effects may lead to decreased fitness (Fonte et al, 2016; Naidoo and Glassom, 2019). In addition, biofilm on surfaces of MPs lead to sinking and facilitate adhesion of metals, such as Cd, Hg, and Cu (Djaoudi et al, 2022; Richard et al, 2019; Turner and Holmes, 2015), and organic pollutants such as polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) (Rochman et al, 2013). Ions within biofilms are less tightly bound and easily desorbed (Kurniawan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Microplastic pollution in aquatic environments may facilitate misfeeding by fish

Yagi

Ono

Kawaguchi

2022

Preprint

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Numerous recent studies have documented ingestion of microplastics (MPs) by many aquatic animals, yet an explanation for misfeeding by fish remains unexplained. Here we tested the hypothesis that biofilm (biofouling) on MP surfaces due to exposure in the aquatic environment facilitates misfeeding in fish. Spherical polystyrene (PS) was cultured for 0 to 22 weeks in a freshwater environment to grow biofilm on the MP. Goldfish were employed in a simple feeding experiment with and without provision of genuine food at ecologically relevant MP concentrations. The absorbance (ABS), which is a proxy for biofilm formation increased exponentially within three weeks of initiation and reached a plateau after approximately five weeks. Although fish did not swallow the MPs, 'capture' occurred when food pellets were in the vicinity and significantly increased in probability with exposure time. Duration of capture also increased significantly with increasing exposure. These results suggest that the drift of MPs in aquatic environments may facilitate fish misidentification of MPs as edible prey.

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Seawater copper content controls biofilm bioaccumulation and microbial community on microplastics

Cited by 22 publications

References 87 publications

Fungal Diversity and Dynamics during Long-Term Immersion of Conventional and Biodegradable Plastics in the Marine Environment

Fungal Diversity and Dynamics during Long-Term Immersion of Conventional and Biodegradable Plastics in the Marine Environment

Synthesis and Isolation of Phenol- and Thiol-Derived Epicatechin Adducts Prepared from Avocado Peel Procyanidins Using Centrifugal Partition Chromatography and the Evaluation of Their Antimicrobial and Antioxidant Activity

Microplastic pollution in aquatic environments may facilitate misfeeding by fish

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