Seaweed micropropagation techniques and their potentials: an overview

Reddy, C. R. K.; Jha, Bhavanath; Fujita, Yuji; Ohno, Masao

doi:10.1007/978-1-4020-9619-8_21

Cited by 24 publications

(33 citation statements)

References 62 publications

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“…The increasing demand for agar worldwide, coupled with short supplies of agarophytes from wild stocks, has led to the development of viable field cultivation methods for their large-scale cultivation in the sea (Critchley 1993). Following the success in large-scale cultivation, cellular biotechnology techniques are also being applied to improve the cultivated germplasm of this important resource (see reviews of Reddy et al 2008aReddy et al , b, 2010. However, these techniques have largely remained underdeveloped and are thus in their nascent stage.…”

Section: Introductionmentioning

confidence: 99%

Optimization of protoplast yields from the red algae Gracilaria dura (C. Agardh) J. Agardh and G. verrucosa (Huds.) Papenfuss

et al. 2010

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This study reports on the optimization of protoplast yield from two important tropical agarophytes Gracilaria dura and Gracilaria verrucosa using different cell-wall-degrading enzymes obtained from commercial sources. The conditions for achieving the highest protoplast yield was investigated by optimizing key parameters such as enzyme combinations and their concentrations, duration of enzyme treatment, enzyme pH, mannitol concentration, and temperature. The significance of each key parameter was also further validated using the statistical central composite design. The enzyme composition with 4% cellulase Onozuka R-10, 2% macerozyme R-10, 0.5% pectolyase, and 100 U agarase, 0.4 M mannitol in seawater (30‰) adjusted to pH 7.5 produced the highest protoplast yields of 3.7±0.7×10 6 cells g −1 fresh wt for G. dura and 1.2±0.78×10 6 cells g −1 fresh wt for G. verrucosa when incubated at 25°C for 4-6 h duration. The young growing tips maximally released the protoplasts having a size of 7-15 μm in G. dura and 15-25 μm in G. verrucosa, mostly from epidermal and upper cortical regions. A few large-size protoplasts of 25-35 μm, presumably from cortical region, were also observed in G. verrucosa.

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Section: Introductionmentioning

confidence: 99%

Optimization of protoplast yields from the red algae Gracilaria dura (C. Agardh) J. Agardh and G. verrucosa (Huds.) Papenfuss

et al. 2010

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“…Although both microalgae and macroalgae can be grown in controlled environments, carotenoid production in macroalgae cultures is still not commercially feasible. The methods employed in seaweed tissue culture involve axenic cell culture techniques enriched with nutrients and growth regulators for explant generation, which generally yields small and slow growing calli (Reddy et al 2008) and can lead to low production yields. On the other hand, microalgae are organisms that have simpler growth requirements.…”

Section: Introductionmentioning

confidence: 99%

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

2015

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Fucoxanthin is a carotenoid that exerts multiple beneficial effects on human health. However, reports comparing microalgae culture conditions and their effect on growth and fucoxanthin production are still limited. Isochrysis galbana and Phaeodactylum tricornutum cultures in different light (62.0, 25.9, 13.5, or 9.1 μmol photons m -2 s -1 ), mixing conditions (1 vvm aeration or 130 rpm agitation), and media compositions (F/2 and Conway medium) were studied for comparison of cellular growth and fucoxanthin production on F/2 medium. I. galbana showed a better adaptation to tested culture conditions in comparison with P. tricornutum, reaching 2.15 × 10 7 ±4.07×10 6 cells mL -1 and a specific growth rate (μ) of 1.12±0.05 day -1 under aerated conditions and 62.0 μmol photons m -2 s -1 light intensity. Fucoxanthin concentration was about 25 % higher in P. tricornutum cultures under 13.5 μmol photons m -2 s -1 light intensity and aerated conditions, but the highest fucoxanthin total production was higher in I. galbana, where 3.32 mg can be obtained from 1 L batch cultures at the 16th day under these conditions. Moreover, higher cell densities (~32.41 %), fucoxanthin concentration (~42.46 %), and total production (~50.68 %) were observed in I. galbana cultures grown in Conway medium, if compared with cultures grown in F/2 medium. The results show that the best growth conditions did not result in the best fucoxanthin production for either microalgae, implying that there is not a direct relationship between cellular growth and fucoxanthin production. Moreover, the results suggest that I. galbana cultures on Conway medium are strong candidates for fucoxanthin production, where 1.2 to 15 times higher fucoxanthin concentration are observed in comparison to macroalgal sources.

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“…Besides, the composition in bioactive compounds strongly varies among species, being affected by numerous factors, such as algae size, age, tissue type, salinity, season, nutrient levels, intensity of herbivory, light intensity and water temperature [52]. In this sense, it is also crucial to implement efficient cultivation techniques, which could not only prevent the overexploitation of natural populations, but also facilitate the development and propagation of genotypes in order to produce high value chemicals for potential application in the pharmaceutical and nutraceutical sectors [166]. …”

Section: Discussionmentioning

confidence: 99%

Bioactive Compounds from Macroalgae in the New Millennium: Implications for Neurodegenerative Diseases

2014

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Marine environment has proven to be a rich source of structurally diverse and complex compounds exhibiting numerous interesting biological effects. Macroalgae are currently being explored as novel and sustainable sources of bioactive compounds for both pharmaceutical and nutraceutical applications. Given the increasing prevalence of different forms of dementia, researchers have been focusing their attention on the discovery and development of new compounds from macroalgae for potential application in neuroprotection. Neuroprotection involves multiple and complex mechanisms, which are deeply related. Therefore, compounds exerting neuroprotective effects through different pathways could present viable approaches in the management of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s. In fact, several studies had already provided promising insights into the neuroprotective effects of a series of compounds isolated from different macroalgae species. This review will focus on compounds from macroalgae that exhibit neuroprotective effects and their potential application to treat and/or prevent neurodegenerative diseases.

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Seaweed micropropagation techniques and their potentials: an overview

Cited by 24 publications

References 62 publications

Optimization of protoplast yields from the red algae Gracilaria dura (C. Agardh) J. Agardh and G. verrucosa (Huds.) Papenfuss

Optimization of protoplast yields from the red algae Gracilaria dura (C. Agardh) J. Agardh and G. verrucosa (Huds.) Papenfuss

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

Bioactive Compounds from Macroalgae in the New Millennium: Implications for Neurodegenerative Diseases

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