Seaweeds: Potential Candidates in Human Colon Cancer Therapy

Pandey, Raghvendra; Singh, Prashant; Shrivastava, Alok Kumar

doi:10.1007/978-3-030-64668-4_13

Cited by 1 publication

(2 citation statements)

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“…The ultimate aim of discovering novel chemotherapeutic strategies is to overcome drug resistance ( 32 ). A number of studies have previously investigated the potential anticancer activity of natural compounds/products and suggested them to be promising sources of new anticancer drugs ( 4 – 13 ). For example, the natural compound S-adenosylmethionine has been found to exert anti-tumor properties, including reduction of cell proliferation, induction of apoptosis, autophagy and inhibition of invasion and metastasis, in various cancer cell types, such as human hepatocellular carcinoma, human breast cancer and head and neck squamous cancer cells, in previous in vitro and in vivo studies ( 33 – 36 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, these treatments are limited by toxicity, adverse events and drug resistance ( 3 ). A number of studies have previously reported that colorectal and colon cancer is negatively associated with dietary factors, including plants, seaweeds, vegetables and fruits, which contain a variety of phytochemicals ( 4 – 6 ). These phytochemicals have been demonstrated to protect cells from damage that leads to cancer ( 7 – 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Dichloromethane fractions of Calystegia soldanella induce S‑phase arrest and apoptosis in HT‑29 human colorectal cancer cells

et al. 2021

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Calystegia soldanella is a halophyte and a perennial herb that grows on coastal sand dunes worldwide. extracts from this plant have been previously revealed to have a variety of bioactive properties in humans. However, their effects on colorectal cancer cells remain poorly understood. in the present study, the potential biological activity of C. soldanella extracts in the colorectal cancer cell line HT-29 was examined. First, five solvent fractions [n-hexane, dichloromethane (dcM), ethyl acetate, n-butanol and water] were obtained from the crude extracts of C. soldanella through an organic solvent extraction method. in particular, the dcM fraction was demonstrated to exert marked dose-and time-dependent inhibitory effects according to results from the cell viability assay. data obtained from the apoptosis assay suggested that the inhibition of HT-29 cell viability induced by dcM treatment was attributed to increased apoptosis. The apoptotic rate was markedly increased in a dose-dependent manner, which was associated with the protein expression levels of apoptosis-related proteins, including increased Fas, Bad and Bax, and decreased pro-caspase-8, Bcl-2, Bcl-xl, pro-caspase-9, pro-caspase-7 and pro-caspase-3. a mitochondrial membrane potential assay demonstrated that more cells became depolarized and the extent of cytochrome c release was markedly increased in a dose-dependent manner in HT-29 cells treated with dcM. in addition, cell cycle analysis confirmed S-phase arrest following DCM fraction treatment, which was associated with decreased protein expression levels of cell cycle-related proteins, such as cyclin a, cdK2, cell division cycle 25 a and cyclin dependent kinase inhibitor 1. Based on these results, the present study suggested that the dcM fraction of the C. soldanella extract can inhibit HT-29 cell viability whilst inducing apoptosis through mitochondrial membrane potential regulation and S-phase arrest. These results also suggested that the dcM fraction has potential anticancer activity in HT-29 colorectal cells. Further research on the composition of the dcM fraction is warranted.

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