SEC–MALLS analysis of wood holocelluloses dissolved in 8 % LiCl/1,3-dimethyl-2-imidazolidinone: challenges and suitable analytical conditions

Ono, Yuko; Hiraoki, Ryoya; Fujisawa, Shuji; Saito, Tsuguyuki; Isogai, Akira

doi:10.1007/s10570-015-0694-z

Cited by 11 publications

(3 citation statements)

References 28 publications

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“…Other SEC attachments and the operation conditions are described elsewhere. 19,20 The dn/dc value of each polymer or cellobiose was obtained from the SEC/MALLS/RI data using the attached software according to eq 1, assuming 100% mass recovery. This method requires accurate injection volumes and sample concentrations, and also needs all sample molecules to be eluted off the column.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The SEC/MALLS/RI analysis system comprising a SEC column (KD-806M, Shodex, Japan), a MALLS detector (DAWN HELEOS-II, λ = 658 nm, Wyatt Technologies, USA), and an RI detector (RID-10A, Shimadzu, Japan), and 1.0% w/v LiCl/DMAc, 0.5% w/v LiCl/DMAc, or 1.0% w/v LiCl/DMI was used as eluents. Other SEC attachments and the operation conditions are described elsewhere. , The d n /d c value of each polymer or cellobiose was obtained from the SEC/MALLS/RI data using the attached software according to eq , assuming 100% mass recovery. This method requires accurate injection volumes and sample concentrations, and also needs all sample molecules to be eluted off the column. where α is a RI detector instrument constant, W injected is the injected mass, ∑Δ v i is the volume of the slice, and V i and V baseline are the RI signal and baseline voltages, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Reliable dn/dc Values of Cellulose, Chitin, and Cellulose Triacetate Dissolved in LiCl/N,N-Dimethylacetamide for Molecular Mass Analysis

et al. 2015

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Freeze-dried microfibrillated cellulose (MFC) was directly dissolved in 8.0% w/w lithium chloride/N,N-dimethylacetamide (LiCl/DMAc), and MFC/LiCl/DMAc solutions with accurate MFC concentrations were prepared. The different MFC solutions were diluted to 1.0% and 0.5% w/v LiCl/DMAc, and subjected to size-exclusion chromatography with multiangle laser-light scattering and refractive index analyses (SEC/MALLS/RI), and off-line RI analysis to determine their refractive index increments (dn/dc). Chitin, cellulose triacetate, a poly(styrene) standard, and cellobiose were used for comparison. Each of the two determination methods gave different dn/dc values for MFC and chitin but similar dn/dc values for cellulose triacetate and poly(styrene). The anomalously small dn/dc values of MFC and chitin were explainable in terms of stable cellulose-LiCl and chitin-LiCl structures (i.e., formation of apparent covalent bonds between hydroxyl groups and LiCl) in the solutions. Thus, the SEC/MALLS/RI method provides reliable molecular mass parameters for cellulose and chitin.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Reliable dn/dc Values of Cellulose, Chitin, and Cellulose Triacetate Dissolved in LiCl/N,N-Dimethylacetamide for Molecular Mass Analysis

et al. 2015

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“…Moreover, MALLS provides structural information, such as radii of gyration . Because of its easy realization, GPC–MALLS has been widely applied to determine absolute molecular weight of starches, proteins, celluloses, synthetic polymers, and so forth.…”

Section: Introductionmentioning

confidence: 99%

Absolute Molecular Weight Determination of Sodium Salt of Methacrylic Acid/Acrylic Acid‐2‐Isooctyl Ester/2‐Acrylamido‐2‐Methylpropanesulfonic Acid Terpolymer by GPC/MALLS

Chang

Liu

Zhang

2016

Bulletin Korean Chem Soc

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The samples of sodium salt of methacrylic acid/acrylic acid‐2‐isooctyl ester/2‐acrylamido‐2‐methylpropanesulfonic acid terpolymer (SSMAA) were synthesized by free radical solution polymerization. The absolute weight‐average molar mass (Mw ) and polydispersity (Mw /Mn ) of the polymers were successfully determined using aqueous‐based gel permeation chromatography coupled with multiangle laser light scattering. The results show that mixed solutions of highly concentrated buffered salt (NaNO3 or NaCl and NaH2PO4 ) and organic solvent modifier (methanol) are needed to eliminate the undesirable interactions between polymer and polymer, and between polymer and stationary phase. Dynamic light scattering was first used to investigate the aggregation behavior of SSMAA in a variety of potential mobile phase compositions and screen the optimum compositions. The Mw and Mw /Mn values of the three samples prepared with different amount of initiator are 13500, 5820, and 2490 g/mol and 1.52, 1.45, and 1.47, respectively.

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Molecular Structure Characterization of Starch

Kajla,

Kumari,

Shobhit

et al. 2024

Methods and Protocols in Food Science

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SEC–MALLS analysis of wood holocelluloses dissolved in 8 % LiCl/1,3-dimethyl-2-imidazolidinone: challenges and suitable analytical conditions

Cited by 11 publications

References 28 publications

Reliable dn/dc Values of Cellulose, Chitin, and Cellulose Triacetate Dissolved in LiCl/N,N-Dimethylacetamide for Molecular Mass Analysis

Reliable dn/dc Values of Cellulose, Chitin, and Cellulose Triacetate Dissolved in LiCl/N,N-Dimethylacetamide for Molecular Mass Analysis

Absolute Molecular Weight Determination of Sodium Salt of Methacrylic Acid/Acrylic Acid‐2‐Isooctyl Ester/2‐Acrylamido‐2‐Methylpropanesulfonic Acid Terpolymer by GPC/MALLS

Molecular Structure Characterization of Starch

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