SEC-MS/MS determination of amino acids from mango fruits and application of the method for studying amino acid perturbations due to post harvest ripening

Chilakala, Shireesha; Mehtab, Vazida; Tallapally, Maheshwari; Vemula, Madhu; Shaikh, Asif Sadiq; Sumana, C.; Upadhyayula, Vijayasarathi

doi:10.1016/j.lwt.2020.110680

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…The LOQ values were between 0.21–2.40 µmol·L −1 , while the LOD values were between 0.07 and 0.73 µmol·L −1 . The ranges of linearity and sensitivity were similar to that of previously published size exclusion chromatography with mass spectrometry methods [ 30 ]. Therefore, the optimized automatic amino acid analyzer method had good linearity and sensitivity for the determination of FAAs and GSH.…”

Section: Resultssupporting

confidence: 79%

Simultaneous Detection and Analysis of Free Amino Acids and Glutathione in Different Shrimp

Jin

Yugan

et al. 2022

Foods

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An amino acid analyzer method for the simultaneous determination of 20 free amino acids (FAAs) and glutathione (GSH) in Penaeus vannamei (PV), Penaeus vannamei, Penaeus hidulis (PH) and Penaeus japonicus (PJ) were developed. The effects of different concentrations of trichloroacetic acid (TCA) and ethanol on the extraction of free amino acids were investigated, and 120 g·L−1 TCA was found to be ideal. The target analytes were eluted in sodium citrate buffer B1 (pH = 3.3) containing 135 mL·L−1 ethanol and 1 mol·L−1 sodium hydroxide (7 mL) and at the optimizing conversion time of sodium citrate buffer B2 (pH = 3.2) and sodium citrate buffer B3 (pH = 4.0) of 5.6 min, and the effective separation was achieved within 29.5 min. The developed method showed good linearity (R2 ≥ 0.9991) in the range of 1–250 µg·mL−1 with good intra-day and inter-day precision (relative standard deviations ≤ 2.38%) and spike recovery (86.42–103.64%). GSH and cysteine were used to identify marine prawn and freshwater shrimp. Hydroxyproline and serine were used to distinguish PV and Macrobrachium nipponense (MN) from others, respectively. The highest content of the total FAAs was found in PV, and principal component analysis revealed that PV had the highest comprehensive score for FAAs and GSH. Arginine was found to have the greatest influence on shrimp flavor.

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Section: Resultssupporting

confidence: 79%

Simultaneous Detection and Analysis of Free Amino Acids and Glutathione in Different Shrimp

Jin

Yugan

et al. 2022

Foods

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“…Amino acids are formed due to various metabolic processes during ripening stages of fruit maturity 44 , and detected in all cvs. at comparable levels ranging from 6.3 to 9.3 µg/mg.…”

Section: Resultsmentioning

confidence: 99%

Valorization potential of Egyptian mango kernel waste product as analyzed via GC/MS metabolites profiling from different cultivars and geographical origins

El-Shabasy,

F. Eissa,

Emam

et al. 2024

Sci Rep

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Increasing attention has been given to mango (Mangifera indica) fruits owing to their characteristic taste, and rich nutritional value. Mango kernels are typically discarded as a major waste product in mango industry, though of potential economic value. The present study aims to outline the first comparison of different mango kernel cvs. originated from different localities alongside Egypt, e.g., Sharqia, Suez, Ismailia, and Giza. Gas chromatography–mass spectroscopy (GC–MS) post silylation analysis revealed that sugars were the major class being detected at 3.5–290.9 µg/mg, with some kernels originating from Sharqia province being the richest amongst other cvs. In consistency with sugar results, sugar alcohols predominated in Sharqia cvs. at 1.3–38.1 µg/mg represented by ribitol, iditol, pinitol, and myo-inositol. No major variation was observed in the fatty acids profile either based on cv. type or localities, with butyl caprylate as a major component in most cvs. identified for the first time in mango. Regarding phenolics, Sedeeq cv. represented the highest level at 18.3 µg/mg and showing distinct variation among cvs. posing phenolics as better classification markers than sugars. Multivariate data analyses (MVA) confirmed that the premium cvs “Aweis and Fons” were less enriched in sugars, i.e., fructose, talose, and glucose compared to the other cvs. Moreover, MVA of Zabdeya cv. collected from three localities revealed clear segregation to be chemically distinct. Sharqia originated mango kernels were rich in sugars (e.g., glucose and fructose), whilst sarcosine esters predominated in other origins.

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“…In total, 821 metabolites were identified, including sugars, organic acids, amino acids, flavones, and others. Principal component analysis (PCA), a multivariate statistical method for transforming the original correlated data into a new set of unrelated variables named principal components (PCs), is widely used for data exploration, pattern recognition, and data visualization [ 16 ]. As shown in Figure 1 , the contributions of PC1, PC2, and PC3 were 34.66%, 14.79%, and 12.95%, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Fruits are important sources of amino acids, which are the building blocks of proteins in the human body. Some essential amino acids cannot be biosynthesized by humans, so they must be obtained from foods [ 16 ]. In total, we found that two rambutans contained high amounts of 19 amino acids, including seven amino acids (Met, Lys, Ile, Leu, Phe, Thr, and Val) that are essential for humans.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Profiling and Potential Taste Biomarkers of Two Rambutans during Maturation

Deng

Guo

et al. 2023

Molecules

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The metabolite-caused taste variation during rambutan maturation is unknown due to a lack of systematic investigation of all components. In this study, three growing stages, including unripe (S1), half-ripe (S2), and full-ripe (S3) BY2 and BY7 rambutans were compared and profiled by UPLC–MS/MS-based widely targeted metabolomics analysis. We demonstrated that the sugar-acid ratios of two rambutans were greatly improved between the S2 and S3 stages. A total of 821 metabolites were identified, including 232, 205, 204, and 12 differential metabolites (DMs) in BY2-S1 vs. BY2-S2, BY2-S2 vs. BY2-S3, BY7-S1 vs. BY7-S2, and BY7-S2 vs. BY7-S3, respectively. A correlation analysis showed that gamma-aminobutyric acid (GABA) could be the sugar-acid ratio biomarker of BY2 rambutan. Methionine (Met), alanine (Ala), and S-methyl-L-cysteine (SMC) could be total amino acid biomarkers of BY2 and BY7 rambutans. In addition, UPLC-MS/MS-based quantitative verification of the above biomarkers exhibited the same variations as metabolomics analysis. This study not only provides useful nutritive information on rambutans but also valuable metabolic data for rambutan breeding strategies.

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SEC-MS/MS determination of amino acids from mango fruits and application of the method for studying amino acid perturbations due to post harvest ripening

Cited by 12 publications

References 22 publications

Simultaneous Detection and Analysis of Free Amino Acids and Glutathione in Different Shrimp

Simultaneous Detection and Analysis of Free Amino Acids and Glutathione in Different Shrimp

Valorization potential of Egyptian mango kernel waste product as analyzed via GC/MS metabolites profiling from different cultivars and geographical origins

Metabolic Profiling and Potential Taste Biomarkers of Two Rambutans during Maturation

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