Sec-O-glucosylhamaudol suppressed inflammatory reaction induced by LPS in RAW264.7 cells through inhibition of NF-κB and MAPKs signaling

Abstract

As a major bioactive compound from the Saposhnikovia divaricata (Turcz.) Schischk, sec-O-glucosylhamaudol (SOG), has been reported to have anti-nociceptive activity and high 5-lipoxygenase (5-LOX) activity. Nevertheless, the mechanism of the potential anti-inflammatory effects of SOG is unclear. The anti-inflammatory impacts of SOG in RAW 264.7 cell lines stimulated by LPS were explored in the present study. It was found that SOG dose-dependently reduced the emergence of inflammation cytokines, such as IL-6 and TNF-α in Raw264.7 murine macrophages stimulated by LPS. Real-time PCR assay demonstrated the SOG dose-dependently inhibited transcription of these cytokines as well. In addition, it was also found that NF-κB activation and MAPKs phosphorylation including p38, JNK and ERK1/2 induced by LPS were suppressed by SOG. Due to its anti-inflammatory activity, our results suggest that SOG might have therapeutic effects on inflammatory disease, such as acute lung injury or rheumatoid arthritis.

Research Progress of Therapeutic Enzymes and Their Derivatives: Based on Herbal Medicinal Products in Rheumatoid Arthritis

“…Also, a major herbal compound named sec-O-glucosylhamaudol (SOG), which is derived from Saposhnikovia divaricata (Turcz.) Schischk, has been reported to have anti-5-LOX activity, suggesting that SOG might have therapeutic effects on inflammatory disease, such as acute lung injury and RA ( Zhao et al, 2016 ; Liu et al, 2020 ). Based on the above findings, targeting the lipoxygenase may be a great therapeutic strategy in treating inflammatory disease including RA and these herbal medicinal products will develop into effective medicines for RA treatment with the deepening and development of research.…”

Dp44mT regulates the levels of inflammatory mediators through blocking NF-κB nuclear translocation in LPS-stimulated RAW 264.7 macrophages

“…In severe patients with COVID-19, infiltration of monocytes and macrophages into lung lesions and high levels of inflammatory response because of the proinflammatory cytokine storm were reported (Zhang et al 2020 ). Inflammation is a complex biological process in which a host identifies substances in or out of the body as non-self-antigens or self-antigens, and is increased by infection with pathogens such as viruses, bacteria, and parasites (Liu et al 2020 ). A moderate inflammatory response helps the host to block the invasion of pathogens, whereas severe inflammatory response contributes to the pathogenesis of various of inflammatory diseases (Nam et al 2017a ; Liu et al 2020 ).…”

“…Inflammation is a complex biological process in which a host identifies substances in or out of the body as non-self-antigens or self-antigens, and is increased by infection with pathogens such as viruses, bacteria, and parasites (Liu et al 2020 ). A moderate inflammatory response helps the host to block the invasion of pathogens, whereas severe inflammatory response contributes to the pathogenesis of various of inflammatory diseases (Nam et al 2017a ; Liu et al 2020 ). Various inflammatory mediators such as proinflammatory cytokines, nitric oxide (NO), adhesion molecules, and prostaglandin E2 (PGE2) are closely associated with the classical clinical symptoms of inflammation such as heat, swelling, pain, redness, and loss of function during the inflammatory process (Hwang et al 2014 ).…”

“…Various inflammatory mediators such as proinflammatory cytokines, nitric oxide (NO), adhesion molecules, and prostaglandin E2 (PGE2) are closely associated with the classical clinical symptoms of inflammation such as heat, swelling, pain, redness, and loss of function during the inflammatory process (Hwang et al 2014 ). Some researchers have reported that activated macrophages are the major inflammatory cells, which arbitrate most molecular and cellular inflammatory networks via production a variety of inflammatory mediators (Mann et al 2018 ; Liu et al 2020 ). In inflammation, macrophages take charge of the phagocytosis, immunomodulation, and antigen presentation through releasing of a variety of cytokines and growth factors (Fujiwara and Kobayashi 2005 ).…”

Sec-O-glucosylhamaudol Inhibits RANKL-induced Osteoclastogenesis Via Repressing 5-LO and AKT/GSK3β Signaling

“…Sec-O-glucosylhamaudol (SOG) (Figure 1a) is an active avonoid compound derived from the root of Saposhnikovia divaricate. In previous studies, SOG was found to have analgesic and anti-in ammatory activities [13][14][15] . In addition, SOG also exhibited high 5-lipoxygenase (5-LO) inhibitory effect 16 .…”