Sec3 knockdown inhibits TGF-β induced epithelial-mesenchymal transition through the down-regulation of Akt phosphorylation in A549 cells

Qian, Xiaohan; Zhou, Xiaoxu; Shentu, Ping; Yao, Yuanfa; Jiao, Demin; Chen, Qingyong; Zhou, Jianying; Xu, Yingke

doi:10.1016/j.bbrc.2019.08.145

Cited by 3 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increasing evidence has confirmed that many novel markers promote tumor progression by activating the TGF‐β pathway 30 , 31 . Recently, increasing numbers of studies have reported that exocytosis-associated proteins may regulate the activation of the TGF‐β pathway, thereby influencing the progression of tumors 32 , 33 . However, the association between SCRN1 and the TGF‐β/Smad3 pathway remains largely unclear.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Xiao,

Zhang,

Zheng

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Secernin-1 (SCRN1) is a regulator of exocytosis in mast cells. Recently, SCRN1 was reported to be correlated with the prognosis of colorectal cancer and gastric cancer, but its functional effects on oral squamous cell carcinoma (OSCC) remain unclear. Our aim was to explore the expression pattern and the migration and invasion effects of the newly identified SCRN1 in OSCC. Western blotting (WB) was performed to measure SCRN1 expression in human OSCC tissue samples and OSCC cell lines. The effects of SCRN1 on OSCC cell proliferation, invasion and migration were analyzed by cell counting kit-8 and Transwell assays. The expression levels of TGF-β, Smad3 and phosphorylated Smad3 (p-Smad3) were measured by WB. The secretion of matrix metalloproteinase (MMP)-2 and MMP-9 was determined by the enzyme-linked immunosorbent assay. The expression of SCRN1 was significantly elevated in OSCC tissues and cell lines. SCRN1 knockdown reduced the expression of TGF-β and p-Smad3 in OSCC cells. TGF-β stimulation promoted proliferation, invasion and migration and enhanced the expression of p-Smad3 and the secretion of MMP9 in SCRN1-knockdown OSCC cell lines. Our study demonstrated that SCRN1 is upregulated in OSCC. Further analyses demonstrated that SCRN1 promotes the proliferation, invasion and migration of OSCC cells via TGF-β/Smad3 signaling.

show abstract

Section: Discussionmentioning

confidence: 99%

Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Xiao,

Zhang,

Zheng

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Increasing evidence has confirmed that many novel markers promote tumor progression by activating the TGF-β pathway [29,30]. Recently, increasing numbers of studies have reported that exocytosis-associated proteins may regulate activation of the TGF-β pathway, thereby influencing the progression of tumors [31,32]. However, the association between SCRN1 and TGFβ/Smad3 pathway remains largely unclear.…”

Section: Discussionmentioning

confidence: 99%

Secernin 1 promotes cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Xiao

Wen²,

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Secernin-1 (SCRN1) is a regulator of exocytosis in mast cells. Recently, SCRN1 has been reported to be correlated with the prognosis of colorectal cancer and gastric cancer but its functional effects on oral squamous cell carcinoma (OSCC) remains unclear. Our aim was to explore the expression pattern and the migration and invasion effects of the newly identified SCRN1 in OSCC. Methods Western blotting (WB) was used to measure SCRN1 expression in human OSCC tissue samples and OSCC cell lines. Then, the effects of SCRN1 on OSCC cell proliferation, invasion and metastasis were analyzed by cell counting kit-8 (CCK-8) and transwell assays. The secretion of matrix metalloproteinase (MMP)-2 and MMP-9 in SCRN1 knockdown OSCC cells and vector cells was investigated by enzyme-linked immunosorbent assay (ELISA). The expression levels of TGF-β, Smad3 and phosphorylated Smad3 (p-Smad3) in SCRN1 knockdown OSCC cells and vector cells were measured by WB. Results The expression of SCRN1 was significantly elevated in the OSCC tissues and cell lines. Furthermore, SCRN1 knockdown attenuated the proliferation, migration and invasion of SCC15 and HSC3 cells. SCRN1 knockdown reduced the secretion of MMP-9 from HSC3 and SCC15 cells, but the secretion of MMP-2 did not obviously change. Additionally, SCRN1 knockdown reduced the expression of TGF-β and p-Smad3 in HSC3 and SCC15 cells. Conclusions Our study demonstrated that SCRN1 is upregulated in OSCC. Further studies demonstrated that SCRN1 promotes proliferation, invasion and metastasis of OSCC cells via TGF-β/Smad3 signaling.

show abstract

“…The greatest change was detected for a highly conserved 45nt micro-exon of the EXOC1 gene, which was increasingly skipped in the SOX10 pos population (ΔΨ = -34.7%, Fig 5F). The encoded protein, EXOC1/SEC3 forms an essential subunit of the evolutionarily conserved exocyst complex involved in vesicle exocytosis, but has also been implicated in regulating cell migration and EMT in other cellular contexts [122][123][124][125] . Figure 5F depicts another micro-exon-skipping event in the CTNND1 gene.…”

Section: Single Cell Analysis Of Neural Plate Border and Lncrna Expre...mentioning

confidence: 99%

CD146-assisted isolation and multi-omics characterisation of human pluripotent stem cell derived cranial Schwann cell precursor-like cells.

Wolvetang,

Balachandran,

Dave

et al. 2023

Preprint

View full text Add to dashboard Cite

Schwann Cell Precursors (SCPs) are multipotent precursor cells and express SOX10, but relatively little is known about the specification and molecular make-up of human SCPs. To address this, we subjected a human SOX10 knock-in reporter iPSC line to a one step cranial Schwann cell precursor differentiation protocol. We show that SOX10-expressing cells acquire the morphology, motility and gene expression of SCP-like cells, and we exemplify that these cells migrate to multiple developing craniofacial structures following injection into early mouse embryos. We next defined the bulk and single-cell transcriptomes, metabolomes, and proteomes of SOX10 expressing human SCP-like cells, revealing gene expression heterogeneity, shifts in splicing events, a reduced dependence on glycolysis, and changes in the expression of cell adhesion proteins, miRNAs and LncRNAs that accompany SCP specification. Discovery proteomics identifies MCAM (CD146) as a cell surface marker that permits the isolation of pure SOX10 expressing human cranial SCP-like cells from human pluripotent stem cell lines subjected to SCP differentiation. We further show that this permits isolation of Down syndrome cranial SCP-like cells that display defects in proliferation. Collectively these data provide a detailed map of the molecular make-up of in vitro generated human cranial Schwann cell precursor-like cells and exemplify the utility of MCAM-sorted cranial SCP-like cells for modelling human neurocristopathies.

show abstract

Sec3 knockdown inhibits TGF-β induced epithelial-mesenchymal transition through the down-regulation of Akt phosphorylation in A549 cells

Cited by 3 publications

References 28 publications

Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Secernin 1 promotes cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

CD146-assisted isolation and multi-omics characterisation of human pluripotent stem cell derived cranial Schwann cell precursor-like cells.

Contact Info

Product

Resources

About